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Evidence for progressive reduction and loss of telocytes in the dermal cellular network of systemic sclerosis.

Manetti M, Guiducci S, Ruffo M, Rosa I, Faussone-Pellegrini MS, Matucci-Cerinic M, Ibba-Manneschi L - J. Cell. Mol. Med. (2013)

Bottom Line: We presently investigated telocyte distribution and features in the skin of SSc patients compared with normal skin.Telocyte damage and loss evolved differently according to SSc subsets and stages, being more rapid and severe in diffuse SSc.Briefly, in human skin telocytes are a distinct stromal cell population.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Histology and Forensic Medicine, University of Florence, I-50134 Florence, Italy. mirkomanetti@yahoo.it

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Limited cutaneous systemic sclerosis (lcSSc) skin, transmission electron microscopy. (A) Early lcSSc. A telocyte displaying an enlarged shape, due to the presence of large vacuoles (v) in its telopodes, surrounds an area of dermal oedema (asterisk). Both normal mitochondria and swollen mitochondria with a clear matrix and few cristae (arrow) are identifiable in the cytoplasm. (B) Advanced lcSSc. A degenerating telocyte entrapped in the fibrotic extracellular matrix shows numerous swollen mitochondria (arrows). The cytoplasm is dark and contains vacuoles and lipofuscinic bodies. (C) Early lcSSc. Telocytes and telopodes displaying a normal morphology are present in the close vicinity of or even in contact with a myofibroblast which shows a large body rich in rough endoplasmic reticulum, mitochondria and myofilaments. Subplasmalemmal focal densities are evident (arrowheads). (D) Early lcSSc. Some telocytes and telopodes with a normal morphology are present around a blood vessel displaying a patent lumen. The vessel basal lamina is markedly thickened (asterisk). (E) Early lcSSc. Normal telocytes with very long and convoluted telopodes surround a perivascular inflammatory infiltrate composed of monocytes and lymphocytes. Telopodes establish cell-to-cell contacts with inflammatory cells (arrowheads). A mast cell is also in contact with telopodes. TC: telocyte; Tp: telopode; My: myofibroblast; E: endothelial cell; Ly: lymphocyte; Mo: monocyte; MC: mast cell. Scale bars are indicated in each panel.
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fig07: Limited cutaneous systemic sclerosis (lcSSc) skin, transmission electron microscopy. (A) Early lcSSc. A telocyte displaying an enlarged shape, due to the presence of large vacuoles (v) in its telopodes, surrounds an area of dermal oedema (asterisk). Both normal mitochondria and swollen mitochondria with a clear matrix and few cristae (arrow) are identifiable in the cytoplasm. (B) Advanced lcSSc. A degenerating telocyte entrapped in the fibrotic extracellular matrix shows numerous swollen mitochondria (arrows). The cytoplasm is dark and contains vacuoles and lipofuscinic bodies. (C) Early lcSSc. Telocytes and telopodes displaying a normal morphology are present in the close vicinity of or even in contact with a myofibroblast which shows a large body rich in rough endoplasmic reticulum, mitochondria and myofilaments. Subplasmalemmal focal densities are evident (arrowheads). (D) Early lcSSc. Some telocytes and telopodes with a normal morphology are present around a blood vessel displaying a patent lumen. The vessel basal lamina is markedly thickened (asterisk). (E) Early lcSSc. Normal telocytes with very long and convoluted telopodes surround a perivascular inflammatory infiltrate composed of monocytes and lymphocytes. Telopodes establish cell-to-cell contacts with inflammatory cells (arrowheads). A mast cell is also in contact with telopodes. TC: telocyte; Tp: telopode; My: myofibroblast; E: endothelial cell; Ly: lymphocyte; Mo: monocyte; MC: mast cell. Scale bars are indicated in each panel.

Mentions: Telocytes were severely reduced in the dermis of SSc patients, and most of them exhibited ultrastructural abnormalities (Figs 7 and 8). However, a few normal telocytes were also present in the majority of SSc patients (Figs 7 and 8). Fibroblasts, myofibroblasts, mast cells and macrophages were present, but only a few fibroblasts showed ultrastructural changes, in particular in advanced dcSSc patients.


Evidence for progressive reduction and loss of telocytes in the dermal cellular network of systemic sclerosis.

Manetti M, Guiducci S, Ruffo M, Rosa I, Faussone-Pellegrini MS, Matucci-Cerinic M, Ibba-Manneschi L - J. Cell. Mol. Med. (2013)

Limited cutaneous systemic sclerosis (lcSSc) skin, transmission electron microscopy. (A) Early lcSSc. A telocyte displaying an enlarged shape, due to the presence of large vacuoles (v) in its telopodes, surrounds an area of dermal oedema (asterisk). Both normal mitochondria and swollen mitochondria with a clear matrix and few cristae (arrow) are identifiable in the cytoplasm. (B) Advanced lcSSc. A degenerating telocyte entrapped in the fibrotic extracellular matrix shows numerous swollen mitochondria (arrows). The cytoplasm is dark and contains vacuoles and lipofuscinic bodies. (C) Early lcSSc. Telocytes and telopodes displaying a normal morphology are present in the close vicinity of or even in contact with a myofibroblast which shows a large body rich in rough endoplasmic reticulum, mitochondria and myofilaments. Subplasmalemmal focal densities are evident (arrowheads). (D) Early lcSSc. Some telocytes and telopodes with a normal morphology are present around a blood vessel displaying a patent lumen. The vessel basal lamina is markedly thickened (asterisk). (E) Early lcSSc. Normal telocytes with very long and convoluted telopodes surround a perivascular inflammatory infiltrate composed of monocytes and lymphocytes. Telopodes establish cell-to-cell contacts with inflammatory cells (arrowheads). A mast cell is also in contact with telopodes. TC: telocyte; Tp: telopode; My: myofibroblast; E: endothelial cell; Ly: lymphocyte; Mo: monocyte; MC: mast cell. Scale bars are indicated in each panel.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig07: Limited cutaneous systemic sclerosis (lcSSc) skin, transmission electron microscopy. (A) Early lcSSc. A telocyte displaying an enlarged shape, due to the presence of large vacuoles (v) in its telopodes, surrounds an area of dermal oedema (asterisk). Both normal mitochondria and swollen mitochondria with a clear matrix and few cristae (arrow) are identifiable in the cytoplasm. (B) Advanced lcSSc. A degenerating telocyte entrapped in the fibrotic extracellular matrix shows numerous swollen mitochondria (arrows). The cytoplasm is dark and contains vacuoles and lipofuscinic bodies. (C) Early lcSSc. Telocytes and telopodes displaying a normal morphology are present in the close vicinity of or even in contact with a myofibroblast which shows a large body rich in rough endoplasmic reticulum, mitochondria and myofilaments. Subplasmalemmal focal densities are evident (arrowheads). (D) Early lcSSc. Some telocytes and telopodes with a normal morphology are present around a blood vessel displaying a patent lumen. The vessel basal lamina is markedly thickened (asterisk). (E) Early lcSSc. Normal telocytes with very long and convoluted telopodes surround a perivascular inflammatory infiltrate composed of monocytes and lymphocytes. Telopodes establish cell-to-cell contacts with inflammatory cells (arrowheads). A mast cell is also in contact with telopodes. TC: telocyte; Tp: telopode; My: myofibroblast; E: endothelial cell; Ly: lymphocyte; Mo: monocyte; MC: mast cell. Scale bars are indicated in each panel.
Mentions: Telocytes were severely reduced in the dermis of SSc patients, and most of them exhibited ultrastructural abnormalities (Figs 7 and 8). However, a few normal telocytes were also present in the majority of SSc patients (Figs 7 and 8). Fibroblasts, myofibroblasts, mast cells and macrophages were present, but only a few fibroblasts showed ultrastructural changes, in particular in advanced dcSSc patients.

Bottom Line: We presently investigated telocyte distribution and features in the skin of SSc patients compared with normal skin.Telocyte damage and loss evolved differently according to SSc subsets and stages, being more rapid and severe in diffuse SSc.Briefly, in human skin telocytes are a distinct stromal cell population.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Histology and Forensic Medicine, University of Florence, I-50134 Florence, Italy. mirkomanetti@yahoo.it

Show MeSH
Related in: MedlinePlus