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Evidence for progressive reduction and loss of telocytes in the dermal cellular network of systemic sclerosis.

Manetti M, Guiducci S, Ruffo M, Rosa I, Faussone-Pellegrini MS, Matucci-Cerinic M, Ibba-Manneschi L - J. Cell. Mol. Med. (2013)

Bottom Line: We presently investigated telocyte distribution and features in the skin of SSc patients compared with normal skin.Telocyte damage and loss evolved differently according to SSc subsets and stages, being more rapid and severe in diffuse SSc.Briefly, in human skin telocytes are a distinct stromal cell population.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Histology and Forensic Medicine, University of Florence, I-50134 Florence, Italy. mirkomanetti@yahoo.it

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Limited cutaneous systemic sclerosis (lcSSc) skin, immunohistochemistry. (A–I) Double immunoenzymatic labelling for CD34 and c-kit with horseradish peroxidase (green) and alkaline phosphatase (red) detection systems respectively. Everywhere in the dermis, telocytes and endothelial cells are CD34-positive, mast cells are c-kit-positive. Melanocytes (A, B, D, G, I) and epithelial cells of sebaceous (D and G) and sweat (F and H) glands are c-kit-immunoreactive. (A–F) Early lcSSc skin. Telocytes are absent from the papillary dermis (A, B, D, double asterisks) and patchily reduced in the reticular dermis. In the reticular dermis, most telocytes are enlarged in shape (C). Telocytes are present around arterioles (E, arrow), nerves (E, arrowhead), hair follicles and sebaceous glands (D, arrows), and eccrine sweat glands (F). (G–I) Advanced lcSSc skin. Telocytes are absent from the papillary dermis and severely reduced in the reticular dermis and around most of the adnexal structures (G). In the deep reticular dermis, telocytes are preserved around sweat glands (H). A normal distribution of telocytes is observed in clinically non-involved lcSSc skin (I). (J–L) Advanced lcSSc skin, double immunofluorescence labelling for CD34 (green) and CD90/Thy-1 (red). Very few telocytes are observed (J and L). Fibroblasts are CD90-positive/CD34-negative (K, L, arrows). A CD90-positive vascular wall-resident stem cell niche is scarcely surrounded by telocytes (L, arrowheads). Scale bars are indicated in each panel.
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fig03: Limited cutaneous systemic sclerosis (lcSSc) skin, immunohistochemistry. (A–I) Double immunoenzymatic labelling for CD34 and c-kit with horseradish peroxidase (green) and alkaline phosphatase (red) detection systems respectively. Everywhere in the dermis, telocytes and endothelial cells are CD34-positive, mast cells are c-kit-positive. Melanocytes (A, B, D, G, I) and epithelial cells of sebaceous (D and G) and sweat (F and H) glands are c-kit-immunoreactive. (A–F) Early lcSSc skin. Telocytes are absent from the papillary dermis (A, B, D, double asterisks) and patchily reduced in the reticular dermis. In the reticular dermis, most telocytes are enlarged in shape (C). Telocytes are present around arterioles (E, arrow), nerves (E, arrowhead), hair follicles and sebaceous glands (D, arrows), and eccrine sweat glands (F). (G–I) Advanced lcSSc skin. Telocytes are absent from the papillary dermis and severely reduced in the reticular dermis and around most of the adnexal structures (G). In the deep reticular dermis, telocytes are preserved around sweat glands (H). A normal distribution of telocytes is observed in clinically non-involved lcSSc skin (I). (J–L) Advanced lcSSc skin, double immunofluorescence labelling for CD34 (green) and CD90/Thy-1 (red). Very few telocytes are observed (J and L). Fibroblasts are CD90-positive/CD34-negative (K, L, arrows). A CD90-positive vascular wall-resident stem cell niche is scarcely surrounded by telocytes (L, arrowheads). Scale bars are indicated in each panel.

Mentions: Telocytes and endothelial cells were CD34-positive, and mast cells were c-kit-positive also in skin biopsies from patients with SSc (Figs 3 and 4). However, a striking reduction in telocytes was found in clinically affected skin of SSc patients, with relevant differences according to disease subsets and stages (Figs 3 and 4).


Evidence for progressive reduction and loss of telocytes in the dermal cellular network of systemic sclerosis.

Manetti M, Guiducci S, Ruffo M, Rosa I, Faussone-Pellegrini MS, Matucci-Cerinic M, Ibba-Manneschi L - J. Cell. Mol. Med. (2013)

Limited cutaneous systemic sclerosis (lcSSc) skin, immunohistochemistry. (A–I) Double immunoenzymatic labelling for CD34 and c-kit with horseradish peroxidase (green) and alkaline phosphatase (red) detection systems respectively. Everywhere in the dermis, telocytes and endothelial cells are CD34-positive, mast cells are c-kit-positive. Melanocytes (A, B, D, G, I) and epithelial cells of sebaceous (D and G) and sweat (F and H) glands are c-kit-immunoreactive. (A–F) Early lcSSc skin. Telocytes are absent from the papillary dermis (A, B, D, double asterisks) and patchily reduced in the reticular dermis. In the reticular dermis, most telocytes are enlarged in shape (C). Telocytes are present around arterioles (E, arrow), nerves (E, arrowhead), hair follicles and sebaceous glands (D, arrows), and eccrine sweat glands (F). (G–I) Advanced lcSSc skin. Telocytes are absent from the papillary dermis and severely reduced in the reticular dermis and around most of the adnexal structures (G). In the deep reticular dermis, telocytes are preserved around sweat glands (H). A normal distribution of telocytes is observed in clinically non-involved lcSSc skin (I). (J–L) Advanced lcSSc skin, double immunofluorescence labelling for CD34 (green) and CD90/Thy-1 (red). Very few telocytes are observed (J and L). Fibroblasts are CD90-positive/CD34-negative (K, L, arrows). A CD90-positive vascular wall-resident stem cell niche is scarcely surrounded by telocytes (L, arrowheads). Scale bars are indicated in each panel.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig03: Limited cutaneous systemic sclerosis (lcSSc) skin, immunohistochemistry. (A–I) Double immunoenzymatic labelling for CD34 and c-kit with horseradish peroxidase (green) and alkaline phosphatase (red) detection systems respectively. Everywhere in the dermis, telocytes and endothelial cells are CD34-positive, mast cells are c-kit-positive. Melanocytes (A, B, D, G, I) and epithelial cells of sebaceous (D and G) and sweat (F and H) glands are c-kit-immunoreactive. (A–F) Early lcSSc skin. Telocytes are absent from the papillary dermis (A, B, D, double asterisks) and patchily reduced in the reticular dermis. In the reticular dermis, most telocytes are enlarged in shape (C). Telocytes are present around arterioles (E, arrow), nerves (E, arrowhead), hair follicles and sebaceous glands (D, arrows), and eccrine sweat glands (F). (G–I) Advanced lcSSc skin. Telocytes are absent from the papillary dermis and severely reduced in the reticular dermis and around most of the adnexal structures (G). In the deep reticular dermis, telocytes are preserved around sweat glands (H). A normal distribution of telocytes is observed in clinically non-involved lcSSc skin (I). (J–L) Advanced lcSSc skin, double immunofluorescence labelling for CD34 (green) and CD90/Thy-1 (red). Very few telocytes are observed (J and L). Fibroblasts are CD90-positive/CD34-negative (K, L, arrows). A CD90-positive vascular wall-resident stem cell niche is scarcely surrounded by telocytes (L, arrowheads). Scale bars are indicated in each panel.
Mentions: Telocytes and endothelial cells were CD34-positive, and mast cells were c-kit-positive also in skin biopsies from patients with SSc (Figs 3 and 4). However, a striking reduction in telocytes was found in clinically affected skin of SSc patients, with relevant differences according to disease subsets and stages (Figs 3 and 4).

Bottom Line: We presently investigated telocyte distribution and features in the skin of SSc patients compared with normal skin.Telocyte damage and loss evolved differently according to SSc subsets and stages, being more rapid and severe in diffuse SSc.Briefly, in human skin telocytes are a distinct stromal cell population.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Histology and Forensic Medicine, University of Florence, I-50134 Florence, Italy. mirkomanetti@yahoo.it

Show MeSH
Related in: MedlinePlus