The RNA binding protein Musashi1 regulates apoptosis, gene expression and stress granule formation in urothelial carcinoma cells.
Bottom Line: Up-regulated mRNAs contained a highly significantly greater number and density of Musashi binding sites.A significant number of up-regulated genes encoded components of stress granules (SGs), an organelle involved in translational regulation and mRNA turnover, and impacting on apoptosis.Accordingly, heat shock induced SG formation was augmented by Musashi1 down-regulation.
Affiliation: Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.Show MeSH
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Mentions: Up-regulation of several cell cycle and cell death-related genes after MSI1 depletion was confirmed by quantitative RT-PCR for CDKN1A/p21, CDKN1B/p27, BNIP3, SPRY2 and HPP1 transcripts (Fig. 4A). Likewise, according to Western blotting, p21CIP1 and p27KIP1 protein levels increased after MSI1 down-regulation in 5637 cells, but notably not in 639v cells (Fig. 4B).
Affiliation: Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.