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The RNA binding protein Musashi1 regulates apoptosis, gene expression and stress granule formation in urothelial carcinoma cells.

Nikpour P, Baygi ME, Steinhoff C, Hader C, Luca AC, Mowla SJ, Schulz WA - J. Cell. Mol. Med. (2010)

Bottom Line: Up-regulated mRNAs contained a highly significantly greater number and density of Musashi binding sites.A significant number of up-regulated genes encoded components of stress granules (SGs), an organelle involved in translational regulation and mRNA turnover, and impacting on apoptosis.Accordingly, heat shock induced SG formation was augmented by Musashi1 down-regulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

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Related in: MedlinePlus

Expression of selected growth regulatory genes in MSI1-siRNA treated cells. (A) Expression of CDKN1A, CDKN1B, BNIP3, HIP and SPRY2 relative to TBP in the bladder cancer cell line 5637 after successful suppression of MSI1 (see first bar on the left) as determined by quantitative RT-PCR. For each gene, relative expression in IR-siRNA treated controls was adjusted to 100. (B) Western blot analysis of p21CIP1 and p27KIP1 expression following MSI1 siRNA treatment in the bladder cancer cell lines 5637 and 639v; α-tubulin was employed as a loading control. Statistically significant differences (P < 0.05) are marked by asterisks.
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fig04: Expression of selected growth regulatory genes in MSI1-siRNA treated cells. (A) Expression of CDKN1A, CDKN1B, BNIP3, HIP and SPRY2 relative to TBP in the bladder cancer cell line 5637 after successful suppression of MSI1 (see first bar on the left) as determined by quantitative RT-PCR. For each gene, relative expression in IR-siRNA treated controls was adjusted to 100. (B) Western blot analysis of p21CIP1 and p27KIP1 expression following MSI1 siRNA treatment in the bladder cancer cell lines 5637 and 639v; α-tubulin was employed as a loading control. Statistically significant differences (P < 0.05) are marked by asterisks.

Mentions: Up-regulation of several cell cycle and cell death-related genes after MSI1 depletion was confirmed by quantitative RT-PCR for CDKN1A/p21, CDKN1B/p27, BNIP3, SPRY2 and HPP1 transcripts (Fig. 4A). Likewise, according to Western blotting, p21CIP1 and p27KIP1 protein levels increased after MSI1 down-regulation in 5637 cells, but notably not in 639v cells (Fig. 4B).


The RNA binding protein Musashi1 regulates apoptosis, gene expression and stress granule formation in urothelial carcinoma cells.

Nikpour P, Baygi ME, Steinhoff C, Hader C, Luca AC, Mowla SJ, Schulz WA - J. Cell. Mol. Med. (2010)

Expression of selected growth regulatory genes in MSI1-siRNA treated cells. (A) Expression of CDKN1A, CDKN1B, BNIP3, HIP and SPRY2 relative to TBP in the bladder cancer cell line 5637 after successful suppression of MSI1 (see first bar on the left) as determined by quantitative RT-PCR. For each gene, relative expression in IR-siRNA treated controls was adjusted to 100. (B) Western blot analysis of p21CIP1 and p27KIP1 expression following MSI1 siRNA treatment in the bladder cancer cell lines 5637 and 639v; α-tubulin was employed as a loading control. Statistically significant differences (P < 0.05) are marked by asterisks.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822633&req=5

fig04: Expression of selected growth regulatory genes in MSI1-siRNA treated cells. (A) Expression of CDKN1A, CDKN1B, BNIP3, HIP and SPRY2 relative to TBP in the bladder cancer cell line 5637 after successful suppression of MSI1 (see first bar on the left) as determined by quantitative RT-PCR. For each gene, relative expression in IR-siRNA treated controls was adjusted to 100. (B) Western blot analysis of p21CIP1 and p27KIP1 expression following MSI1 siRNA treatment in the bladder cancer cell lines 5637 and 639v; α-tubulin was employed as a loading control. Statistically significant differences (P < 0.05) are marked by asterisks.
Mentions: Up-regulation of several cell cycle and cell death-related genes after MSI1 depletion was confirmed by quantitative RT-PCR for CDKN1A/p21, CDKN1B/p27, BNIP3, SPRY2 and HPP1 transcripts (Fig. 4A). Likewise, according to Western blotting, p21CIP1 and p27KIP1 protein levels increased after MSI1 down-regulation in 5637 cells, but notably not in 639v cells (Fig. 4B).

Bottom Line: Up-regulated mRNAs contained a highly significantly greater number and density of Musashi binding sites.A significant number of up-regulated genes encoded components of stress granules (SGs), an organelle involved in translational regulation and mRNA turnover, and impacting on apoptosis.Accordingly, heat shock induced SG formation was augmented by Musashi1 down-regulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Show MeSH
Related in: MedlinePlus