Peritoneal repairing cells: a type of bone marrow derived progenitor cells involved in mesothelial regeneration.
Bottom Line: This was observed in the injured area as well as in the surrounding not-injured peritoneal surfaces.We suggest that PRC constitute a type of monocyte-derived cells, closely related with the tissue-repairing cells known as 'fibrocytes' and specifically involved in peritoneal reparation.Thus, our results constitute a synthesis of the different scenarios hitherto proposed about peritoneal regeneration, particularly recruitment of circulating progenitor cells and adhesion of free-floating coelomic cells.
Affiliation: Department of Animal Biology, Faculty of Science, University of Málaga, Málaga, Spain.Show MeSH
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Mentions: Colocalization of mesothelial and leucocytic markers was frequent in primary culture of cells obtained from peritoneal lavages 48 hrs after injury, either from mice with GFP-expressing bone marrow or from normal mice (Fig. 3). The colocalization was always found in large, spindle-shaped cells. Cytokeratin showed a characteristic perinuclear dot-like staining pattern (Fig. 3A, colocalization with CD45). This pattern is frequent in primary cultures of mesothelial cells, in which the cytokeratin cytoskeleton seems to collapse, as shown in Figure 3(B), where some mesothelial cells show also the perinuclear dot like pattern whereas others show a still well-developed cytokeratin cytoskeleton (arrowhead in Fig. 3B). CD68 showed an intracytoplasmic pattern and colocalization with mesothelin (Fig. 3C). In peritoneal cells obtained from mice with GFP-expressing bone marrow, GFP colocalized with both mesothelin and cytokeratin in some cells (arrowheads in Fig. 3D) whereas others were GFP–/cytokeratin+/mesothelin+ (white arrows in Fig. 3D). GFP also colocalized with the fibroblastic marker FSP1 in part of the cells (Fig. 3E, E’). Negative control was incubated with isotype IgG and the same secondary antibodies than in the other slides (Fig. 3F).
Affiliation: Department of Animal Biology, Faculty of Science, University of Málaga, Málaga, Spain.