Identification of a novel antigen cross-presenting cell type in spleen.
Bottom Line: L-DC display a myeloid dendritic-like phenotype equivalent to LTC-DC as CD11c(lo) CD11b(hi) MHC-II(-) CD8α(-) cells, distinct by high accessibility and endocytic capacity for blood-borne antigen.However, they have weak ability to stimulate CD4(+) T cells in antigen-specific responses.Evidence is presented here for a novel DC type produced by in vitro haematopoiesis which has distinct antigen-presenting potential and reflects a DC subset present also in vivo in spleen.
Affiliation: Research School of Biology, The Australian National University, Canberra, ACT, Australia.Show MeSH
Related in: MedlinePlus
Mentions: CD8α+ cDC are highly cross-presenting cells both in vitro  and in vivo [2, 17]. We therefore compared L-DC with CD8α+ cDC for cross-presenting ability in vitro. APC subsets were isolated and pulsed with OVA or HEL and co-cultured with purified OT-I CD8+ T cells in the presence and absence of LPS as an activator. L-DC were strong activators of OT-I CD8+ T cells but only after LPS activation. The cross-presenting capacity of CD8α+ cDC was very strong, and increased after activation with LPS (Fig. 6A). The main phenotypic effect of LPS activation on L-DC after culture in vitro maps to up-regulation of CD86, which is not expressed on freshly isolated cells (Fig 5; all data not shown).
Affiliation: Research School of Biology, The Australian National University, Canberra, ACT, Australia.