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Taxol, a microtubule stabilizer, prevents ischemic ventricular arrhythmias in rats.

Xiao J, Cao H, Liang D, Liu Y, Zhang H, Zhao H, Liu Y, Li J, Yan B, Peng L, Zhou Z, Chen YH - J. Cell. Mol. Med. (2010)

Bottom Line: However, the effects of Taxol, a common microtubule stabilizer, are still unknown in ischemic ventricular arrhythmias.Our data showed that Taxol (0.1, 0.3 and 1 μM) effectively reduced the number of ventricular premature beats and the incidence and duration of ventricular tachycardia.The infarct size was also significantly reduced by Taxol (1 μM).

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Arrhythmias, Ministry of Education, East Hospital, Tongji University School of Medicine, Shanghai, China.

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Related in: MedlinePlus

Taxol decreases the rise in [Ca2+]i and preserves the amplitude and decay time of Ca2+ transients in ischemia. (A) [Ca2+]i changes over time. Values represent the mean ± S.E. (n = 50 per group). *P < 0.05 versus control group, □P < 0.05 versus control group and ischemia group. (B) Typical pictures of Ca2+ transients at the end of the experiment. (C) The amplitude of the Ca2+ transients changes over time. Values represent the mean ± S.E. (n = 50 per group). *P < 0.05 versus control group, □P < 0.05 versus control group and ischemia group. (D) The decay time of the Ca2+ transients changes over time. Values represent the mean ± S.E. (n = 50 per group). *P < 0.05 versus control group, □P < 0.05 versus control group and ischemia group.
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fig04: Taxol decreases the rise in [Ca2+]i and preserves the amplitude and decay time of Ca2+ transients in ischemia. (A) [Ca2+]i changes over time. Values represent the mean ± S.E. (n = 50 per group). *P < 0.05 versus control group, □P < 0.05 versus control group and ischemia group. (B) Typical pictures of Ca2+ transients at the end of the experiment. (C) The amplitude of the Ca2+ transients changes over time. Values represent the mean ± S.E. (n = 50 per group). *P < 0.05 versus control group, □P < 0.05 versus control group and ischemia group. (D) The decay time of the Ca2+ transients changes over time. Values represent the mean ± S.E. (n = 50 per group). *P < 0.05 versus control group, □P < 0.05 versus control group and ischemia group.

Mentions: Microtubules are important regulators of Ca2+ handling in the heart, and abnormal calcium homeostasis plays a causal role in the genesis of arrhythmias [5, 23, 24]. Therefore, the effects of Taxol on calcium homeostasis in the condition of simulated ischemia were studied. During the condition of simulated ischemia, [Ca2+]i was increased by 62% (P < 0.05) (Fig. 4A). With 0.1, 0.3 and 1 μM Taxol, the rise in [Ca2+]i was reduced by 23%, 25% and 30%, respectively (P < 0.05) (Fig. 4A). Typical pictures of Ca2+ transients are shown in Figure 4B. During the condition of simulated ischemia, the amplitude of transient decreased by 42%, whereas the decrease was only 13% with Taxol treatment (P < 0.05) (Fig. 4C). During the condition of simulated ischemia, the decay time of the Ca2+ transients was 2.52 times that of the control, while Taxol increased the decay time by 0.59 times (P < 0.05) (Fig. 4D).


Taxol, a microtubule stabilizer, prevents ischemic ventricular arrhythmias in rats.

Xiao J, Cao H, Liang D, Liu Y, Zhang H, Zhao H, Liu Y, Li J, Yan B, Peng L, Zhou Z, Chen YH - J. Cell. Mol. Med. (2010)

Taxol decreases the rise in [Ca2+]i and preserves the amplitude and decay time of Ca2+ transients in ischemia. (A) [Ca2+]i changes over time. Values represent the mean ± S.E. (n = 50 per group). *P < 0.05 versus control group, □P < 0.05 versus control group and ischemia group. (B) Typical pictures of Ca2+ transients at the end of the experiment. (C) The amplitude of the Ca2+ transients changes over time. Values represent the mean ± S.E. (n = 50 per group). *P < 0.05 versus control group, □P < 0.05 versus control group and ischemia group. (D) The decay time of the Ca2+ transients changes over time. Values represent the mean ± S.E. (n = 50 per group). *P < 0.05 versus control group, □P < 0.05 versus control group and ischemia group.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3822629&req=5

fig04: Taxol decreases the rise in [Ca2+]i and preserves the amplitude and decay time of Ca2+ transients in ischemia. (A) [Ca2+]i changes over time. Values represent the mean ± S.E. (n = 50 per group). *P < 0.05 versus control group, □P < 0.05 versus control group and ischemia group. (B) Typical pictures of Ca2+ transients at the end of the experiment. (C) The amplitude of the Ca2+ transients changes over time. Values represent the mean ± S.E. (n = 50 per group). *P < 0.05 versus control group, □P < 0.05 versus control group and ischemia group. (D) The decay time of the Ca2+ transients changes over time. Values represent the mean ± S.E. (n = 50 per group). *P < 0.05 versus control group, □P < 0.05 versus control group and ischemia group.
Mentions: Microtubules are important regulators of Ca2+ handling in the heart, and abnormal calcium homeostasis plays a causal role in the genesis of arrhythmias [5, 23, 24]. Therefore, the effects of Taxol on calcium homeostasis in the condition of simulated ischemia were studied. During the condition of simulated ischemia, [Ca2+]i was increased by 62% (P < 0.05) (Fig. 4A). With 0.1, 0.3 and 1 μM Taxol, the rise in [Ca2+]i was reduced by 23%, 25% and 30%, respectively (P < 0.05) (Fig. 4A). Typical pictures of Ca2+ transients are shown in Figure 4B. During the condition of simulated ischemia, the amplitude of transient decreased by 42%, whereas the decrease was only 13% with Taxol treatment (P < 0.05) (Fig. 4C). During the condition of simulated ischemia, the decay time of the Ca2+ transients was 2.52 times that of the control, while Taxol increased the decay time by 0.59 times (P < 0.05) (Fig. 4D).

Bottom Line: However, the effects of Taxol, a common microtubule stabilizer, are still unknown in ischemic ventricular arrhythmias.Our data showed that Taxol (0.1, 0.3 and 1 μM) effectively reduced the number of ventricular premature beats and the incidence and duration of ventricular tachycardia.The infarct size was also significantly reduced by Taxol (1 μM).

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Arrhythmias, Ministry of Education, East Hospital, Tongji University School of Medicine, Shanghai, China.

Show MeSH
Related in: MedlinePlus