Mechanisms of the protective effects of BMSCs promoted by TMDR with heparinized bFGF-incorporated stent in pig model of acute myocardial ischemia.
Bottom Line: Autologous BMSCs were transplanted into the ischemic region in C group or around the channels in TSC group.Expression of von Willebrand factor, vascular endothelial growth factor, interleukin-1β, transforming growth factor-β(3) , cell proliferation and apoptosis, histological and morphological analyses, myocardial remodelling and cardiac function were evaluated at different time-points.Six weeks after the operation, the above indices were significantly improved in TSC group compared with others (P < 0.05), though C and TS groups also showed better results than the control group (P < 0.05).
Affiliation: Department of Surgery, Tianjin Medical University Cardiovascular Clinical College & TEDA International Cardiovascular Hospital, Tianjin, China.Show MeSH
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Mentions: No differences in EDV, ESV and local left ventricular wall thickness were found among the four groups prior to the treatment. Six weeks after treatment, no difference was found in local left ventricular wall thickness between C group (2.4 ± 0.1 mm, P = 1) and control group (2.3 ± 0.1 mm). However, a significant increase was detected in TS group (3.3 ± 0.2 mm, P < 0.001) when compared with C and control groups, and there was a further increase in TSC group (4.3 ± 0.2 mm, P < 0.001) compared with TS groups (Fig. 6B). EDV significantly decreased in TSC group (134 ± 8 ml) as compared with control group (167 ± 9 ml, P < 0.001), C group (153 ± 5 ml, P = 0.001) and TS group (147 ± 7 ml, P = 0.034), although there was smaller EDV in C group (P = 0.031) and TS group (P = 0.001) than in the control group. Notably, there had been remarkable increases in all groups at self-comparison with pre-treatment (control group: 129 ± 7 ml, P < 0.001; C group: 128 ± 9 ml, P < 0.001; TS group: 129 ± 8 ml, P < 0.001; TSC group: 126 ± 10 ml, P = 0.006, Fig. 6C). Similar results were obtained for ESV. (Fig. 6D) Although EF was significantly decreased in all groups compared with pre-treatment (64 ± 2%, P < 0.05), there was a significant increase in TSC group (59 ± 2%) compared with control group (52 ± 2%, P < 0.001), C group (53 ± 2%, P < 0.001) and TS group (54 ± 3%, P = 0.006, Fig. 6E).
Affiliation: Department of Surgery, Tianjin Medical University Cardiovascular Clinical College & TEDA International Cardiovascular Hospital, Tianjin, China.