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miR-193 expression differentiates telocytes from other stromal cells.

Cismasiu VB, Radu E, Popescu LM - J. Cell. Mol. Med. (2011)

Bottom Line: In order to define a microRNA (miR) signature in cardiac TCs, we have found that miR-193 is differentially expressed between TCs and other interstitial cells.In addition, the miRs which are specific to CMC and other muscle cells (e.g. miR-133a, miR-208a) are absent in TCs.Overall the data reinforce the view that TCs are a particular type of interstitial (mesenchymal) cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania.

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Expression levels of miRs specifically up-regulated in CMC (A) or in both cultured cardiac interstitial cells and NIH3T3 fibroblasts (B). The relative expression was determined by real-time PCR. RNU43 snoRNA and U6 snRNA were used as normalizing controls. The error bars are mean + S.D. (n = 3) and the data are representative for two replicate experiments. CMCs: cardiomyocytes; CCCs: cultured cardiac cells; NIH3T3: fibroblasts.
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fig02: Expression levels of miRs specifically up-regulated in CMC (A) or in both cultured cardiac interstitial cells and NIH3T3 fibroblasts (B). The relative expression was determined by real-time PCR. RNU43 snoRNA and U6 snRNA were used as normalizing controls. The error bars are mean + S.D. (n = 3) and the data are representative for two replicate experiments. CMCs: cardiomyocytes; CCCs: cultured cardiac cells; NIH3T3: fibroblasts.

Mentions: In order to validate our method in terms of specificity and sensitivity, we have tested the expression of several miRs known to be preferentially expressed in different types of cells within cardiac tissue (Fig. 2). Thus, the CMC-specific miRs, miR-133a and miR-208a, show high level of expression in the samples enriched in CMC (Fig. 2A), whereas miR-21, miR-29 and miR-199a-5p are preferentially expressed in cultured cardiac cells (CCC) and in fibroblasts (Fig. 2B). Noteworthy, miR-193 is expressed at lower level in CCC than in 3T3 fibroblasts, suggesting that, besides of cardiac fibroblasts, the cultures may contain cells where this miR is down-regulated (Fig. 2B).


miR-193 expression differentiates telocytes from other stromal cells.

Cismasiu VB, Radu E, Popescu LM - J. Cell. Mol. Med. (2011)

Expression levels of miRs specifically up-regulated in CMC (A) or in both cultured cardiac interstitial cells and NIH3T3 fibroblasts (B). The relative expression was determined by real-time PCR. RNU43 snoRNA and U6 snRNA were used as normalizing controls. The error bars are mean + S.D. (n = 3) and the data are representative for two replicate experiments. CMCs: cardiomyocytes; CCCs: cultured cardiac cells; NIH3T3: fibroblasts.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822620&req=5

fig02: Expression levels of miRs specifically up-regulated in CMC (A) or in both cultured cardiac interstitial cells and NIH3T3 fibroblasts (B). The relative expression was determined by real-time PCR. RNU43 snoRNA and U6 snRNA were used as normalizing controls. The error bars are mean + S.D. (n = 3) and the data are representative for two replicate experiments. CMCs: cardiomyocytes; CCCs: cultured cardiac cells; NIH3T3: fibroblasts.
Mentions: In order to validate our method in terms of specificity and sensitivity, we have tested the expression of several miRs known to be preferentially expressed in different types of cells within cardiac tissue (Fig. 2). Thus, the CMC-specific miRs, miR-133a and miR-208a, show high level of expression in the samples enriched in CMC (Fig. 2A), whereas miR-21, miR-29 and miR-199a-5p are preferentially expressed in cultured cardiac cells (CCC) and in fibroblasts (Fig. 2B). Noteworthy, miR-193 is expressed at lower level in CCC than in 3T3 fibroblasts, suggesting that, besides of cardiac fibroblasts, the cultures may contain cells where this miR is down-regulated (Fig. 2B).

Bottom Line: In order to define a microRNA (miR) signature in cardiac TCs, we have found that miR-193 is differentially expressed between TCs and other interstitial cells.In addition, the miRs which are specific to CMC and other muscle cells (e.g. miR-133a, miR-208a) are absent in TCs.Overall the data reinforce the view that TCs are a particular type of interstitial (mesenchymal) cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania.

Show MeSH
Related in: MedlinePlus