Infiltration of myeloid cells into decidua is a critical early event in the labour cascade and post-partum uterine remodelling.
Bottom Line: Flow cytometry revealed a significant (P < 0.05) increase in decidual Macs prior to TL; M and N numbers increased during TL and further increased during PP, which correlated with immunohistochemistry data.PP period following TL and PTL was associated with further up-regulation of multiple cytokines/chemokines (P < 0.05).Our data suggest a programme of myeloid cells involvement in parturition with the pre-partum influx of Macs into the decidua contributing to the progression of labour, whereas the later influx of M and N contribute to PP decidual involution.
Affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada. firstname.lastname@example.orgShow MeSH
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Mentions: The expression of multiple pro- and anti-inflammatory genes were evaluated by Real-Time PCR during late gestation, TL, and PP. Pro-inflammatory cytokines tumour necrosis factor-α (Tnf), interleukin (Il)1β (Il1b), Il6 and Il12 as well as two major neutrophil chemoattractants (C-X-C motif) ligand 1 (Cxcl1, also known as Neutrophil-activating protein 3 (NAP-3) or KC), Cxcl2 (also known as macrophage inflammatory protein 2-alpha, Mip2a) and monocyte chemoattractant (C-C motif) Ligand 2 (Ccl2, also known as monocyte chemoattractant protein-1, Mcp1) mRNA levels all were significantly up-regulated during labour and PP (Fig. 3, Table 2). Ccl4 (also known as Macrophage inflammatory protein 1 beta, Mip1b), Ccl3 (also known as Macrophage inflammatory protein 1 alpha, Mip1a) and anti-inflammatory cytokine Il10 genes were also increased during TL but significance was not reached due to a high variability between samples (Fig. 3A). Transcript levels of growth factor Csf2 (granulocyte–macrophage colony-stimulating factor) regulating neutrophil/monocyte differentiation increased in decidua PP 2–6 hrs after parturition (Fig. 3B). The expression of corresponding cytokine proteins partially confirmed changes in cytokine transcripts. Using multiplex protein assay we detected a significant increase in decidual IL1b and IL6 pro-inflammatory cytokines and Ccl2 chemokine during TL and PP (P < 0.05, Fig. 4, Table 3). CXCL1 protein was only increased PP (P < 0.05) whereas no change in the protein level of TNFα, IL10 and CSF2 was observed (Fig. 4, Table 3).
Affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada. email@example.com