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Fatty acid binding protein 4 in circulating leucocytes reflects atherosclerotic lesion progression in Apoe(-/-) mice.

Agardh HE, Gertow K, Salvado DM, Hermansson A, van Puijvelde GH, Hansson GK, n-Berne GP, Gabrielsen A - J. Cell. Mol. Med. (2013)

Bottom Line: Interestingly, the transcript FABP4 correlated significantly with lesion size, further supporting a disease associated increase.Using human circulating leucocytes, we confirmed the presence of FABP4 protein in neutrophils and monocytes.In conclusion, we have showed that cellular levels of FABP4 in circulating leucocytes associate with lesion development in the experimental Apoe(-/-) model.

View Article: PubMed Central - PubMed

Affiliation: Experimental Cardiovascular Research, Department of Medicine, Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden. Hanna.agardh@ki.se

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FABP4 mRNA levels in circulating leucocytes. (A) Gene arrays on circulating leucocytes show increased mRNA levels of FABP4 in 20 and 32 weeks old Apoe−/−, as compared to 12 weeks old Apoe−/−. C57BL/6, 85 weeks of age, were used as controls. One array per age group was run on pooled RNA (n = 6–8) from circulating leucocytes. (B) mRNA levels of FABP4 in circulating leucocytes from Apoe−/− and C57Bl6 (8 weeks of age) measured by RT-PCR TaqMan (n = 10–11).
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fig01: FABP4 mRNA levels in circulating leucocytes. (A) Gene arrays on circulating leucocytes show increased mRNA levels of FABP4 in 20 and 32 weeks old Apoe−/−, as compared to 12 weeks old Apoe−/−. C57BL/6, 85 weeks of age, were used as controls. One array per age group was run on pooled RNA (n = 6–8) from circulating leucocytes. (B) mRNA levels of FABP4 in circulating leucocytes from Apoe−/− and C57Bl6 (8 weeks of age) measured by RT-PCR TaqMan (n = 10–11).

Mentions: Gene expression profiles in leucocytes from atherosclerotic Apoe−/− mice were analysed to identify if any atherosclerosis-related changes could be detected in circulating cells. The atherosclerotic mice were investigated at three different time-points, 12, 20 and 32 weeks of age, to cover different stages of plaque development. To adjust for any changes correlating to ageing rather than atherosclerosis, the non-atherosclerotic C57BL/6 mouse was used as control. When comparing the transcript profiles between Apoe−/− and control mice, we revealed FABP4 to be the most changed transcript in the circulating leucocytes (Fig. 1A). The transcript level of FABP4 between young (8 weeks old) Apoe−/− and control mice did not differ, as measured by real-time RT-PCR (Fig. 1B). Neither, ALT, CKMB or CRSC differed between the mice groups, indicating normal status of liver, heart and kidney (data not shown).


Fatty acid binding protein 4 in circulating leucocytes reflects atherosclerotic lesion progression in Apoe(-/-) mice.

Agardh HE, Gertow K, Salvado DM, Hermansson A, van Puijvelde GH, Hansson GK, n-Berne GP, Gabrielsen A - J. Cell. Mol. Med. (2013)

FABP4 mRNA levels in circulating leucocytes. (A) Gene arrays on circulating leucocytes show increased mRNA levels of FABP4 in 20 and 32 weeks old Apoe−/−, as compared to 12 weeks old Apoe−/−. C57BL/6, 85 weeks of age, were used as controls. One array per age group was run on pooled RNA (n = 6–8) from circulating leucocytes. (B) mRNA levels of FABP4 in circulating leucocytes from Apoe−/− and C57Bl6 (8 weeks of age) measured by RT-PCR TaqMan (n = 10–11).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3822593&req=5

fig01: FABP4 mRNA levels in circulating leucocytes. (A) Gene arrays on circulating leucocytes show increased mRNA levels of FABP4 in 20 and 32 weeks old Apoe−/−, as compared to 12 weeks old Apoe−/−. C57BL/6, 85 weeks of age, were used as controls. One array per age group was run on pooled RNA (n = 6–8) from circulating leucocytes. (B) mRNA levels of FABP4 in circulating leucocytes from Apoe−/− and C57Bl6 (8 weeks of age) measured by RT-PCR TaqMan (n = 10–11).
Mentions: Gene expression profiles in leucocytes from atherosclerotic Apoe−/− mice were analysed to identify if any atherosclerosis-related changes could be detected in circulating cells. The atherosclerotic mice were investigated at three different time-points, 12, 20 and 32 weeks of age, to cover different stages of plaque development. To adjust for any changes correlating to ageing rather than atherosclerosis, the non-atherosclerotic C57BL/6 mouse was used as control. When comparing the transcript profiles between Apoe−/− and control mice, we revealed FABP4 to be the most changed transcript in the circulating leucocytes (Fig. 1A). The transcript level of FABP4 between young (8 weeks old) Apoe−/− and control mice did not differ, as measured by real-time RT-PCR (Fig. 1B). Neither, ALT, CKMB or CRSC differed between the mice groups, indicating normal status of liver, heart and kidney (data not shown).

Bottom Line: Interestingly, the transcript FABP4 correlated significantly with lesion size, further supporting a disease associated increase.Using human circulating leucocytes, we confirmed the presence of FABP4 protein in neutrophils and monocytes.In conclusion, we have showed that cellular levels of FABP4 in circulating leucocytes associate with lesion development in the experimental Apoe(-/-) model.

View Article: PubMed Central - PubMed

Affiliation: Experimental Cardiovascular Research, Department of Medicine, Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden. Hanna.agardh@ki.se

Show MeSH
Related in: MedlinePlus