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Molecular conformation changes along the malignancy revealed by optical nanosensors.

Cinta Pinzaru S, Falamas A, Dehelean CA - J. Cell. Mol. Med. (2013)

Bottom Line: Sensitive information originating from surrounding native biological molecules is provided by the tissue SERS spectra obtained either with visible or NIR laser line.Labelled nanoparticles introduced systematic differences in tissue response compared with unlabelled ones, suggesting that the label functional groups tag specific tissue components revealed by proteins or nucleic acids bands.The results obtained here open perspectives in applied plasmonic nanoparticles and SERS for the early cancer diagnostic based on the appropriate spectral databank.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Physics, Babes-Bolyai University, Cluj-Napoca, Romania. simona.cinta@phys.ubbcluj.ro

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Related in: MedlinePlus

Balb/c mice and chemical carcinoma: (A) macroscopic image and (B) histological evaluation of skin (week 14) HE ×400, presence of oedematous dermis, hyperemiated blood vessels and chronically inflammatory infiltrate–strong inflammatory process and C skin subepithelial carcinoma C57BL/6J and B16 4A5 melanoma: (C) macroscopic image, (D) histological evaluation, HE ×400, diffusion of melanoma on skin level, (E) S100 immunohistological confirmation, Protein S-100 immunostaining malignant melanoma (×400, EnVision) and (F) melan-A immunohistological confirmation of skin melanoma, Positive focal reaction for melan-A in malignant melanoma (×200, EnVision).
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fig01: Balb/c mice and chemical carcinoma: (A) macroscopic image and (B) histological evaluation of skin (week 14) HE ×400, presence of oedematous dermis, hyperemiated blood vessels and chronically inflammatory infiltrate–strong inflammatory process and C skin subepithelial carcinoma C57BL/6J and B16 4A5 melanoma: (C) macroscopic image, (D) histological evaluation, HE ×400, diffusion of melanoma on skin level, (E) S100 immunohistological confirmation, Protein S-100 immunostaining malignant melanoma (×400, EnVision) and (F) melan-A immunohistological confirmation of skin melanoma, Positive focal reaction for melan-A in malignant melanoma (×200, EnVision).

Mentions: Formalin-fixed tumour sections were H & E stained and analysed as histology samples and by application of S-100 and melan-A methods. Balb/c skin (week 14, Fig. 1A) revealed strong inflammatory process, the presence of oedematous dermis, hyperemiated blood vessels and chronically inflammatory infiltrate. The C57BL/6J mouse (B16 4A5 melanoma, Fig. 1C and D) revealed the diffusion of melanoma on skin level based on the E.S100 immunohistological confirmation.


Molecular conformation changes along the malignancy revealed by optical nanosensors.

Cinta Pinzaru S, Falamas A, Dehelean CA - J. Cell. Mol. Med. (2013)

Balb/c mice and chemical carcinoma: (A) macroscopic image and (B) histological evaluation of skin (week 14) HE ×400, presence of oedematous dermis, hyperemiated blood vessels and chronically inflammatory infiltrate–strong inflammatory process and C skin subepithelial carcinoma C57BL/6J and B16 4A5 melanoma: (C) macroscopic image, (D) histological evaluation, HE ×400, diffusion of melanoma on skin level, (E) S100 immunohistological confirmation, Protein S-100 immunostaining malignant melanoma (×400, EnVision) and (F) melan-A immunohistological confirmation of skin melanoma, Positive focal reaction for melan-A in malignant melanoma (×200, EnVision).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3822590&req=5

fig01: Balb/c mice and chemical carcinoma: (A) macroscopic image and (B) histological evaluation of skin (week 14) HE ×400, presence of oedematous dermis, hyperemiated blood vessels and chronically inflammatory infiltrate–strong inflammatory process and C skin subepithelial carcinoma C57BL/6J and B16 4A5 melanoma: (C) macroscopic image, (D) histological evaluation, HE ×400, diffusion of melanoma on skin level, (E) S100 immunohistological confirmation, Protein S-100 immunostaining malignant melanoma (×400, EnVision) and (F) melan-A immunohistological confirmation of skin melanoma, Positive focal reaction for melan-A in malignant melanoma (×200, EnVision).
Mentions: Formalin-fixed tumour sections were H & E stained and analysed as histology samples and by application of S-100 and melan-A methods. Balb/c skin (week 14, Fig. 1A) revealed strong inflammatory process, the presence of oedematous dermis, hyperemiated blood vessels and chronically inflammatory infiltrate. The C57BL/6J mouse (B16 4A5 melanoma, Fig. 1C and D) revealed the diffusion of melanoma on skin level based on the E.S100 immunohistological confirmation.

Bottom Line: Sensitive information originating from surrounding native biological molecules is provided by the tissue SERS spectra obtained either with visible or NIR laser line.Labelled nanoparticles introduced systematic differences in tissue response compared with unlabelled ones, suggesting that the label functional groups tag specific tissue components revealed by proteins or nucleic acids bands.The results obtained here open perspectives in applied plasmonic nanoparticles and SERS for the early cancer diagnostic based on the appropriate spectral databank.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Physics, Babes-Bolyai University, Cluj-Napoca, Romania. simona.cinta@phys.ubbcluj.ro

Show MeSH
Related in: MedlinePlus