Clinical relevance and functional implications for human leucocyte antigen-g expression in non-small-cell lung cancer.
Bottom Line: HLA-G has been documented both in establishment of anti-tumour immune responses and in tumour evasion.Patient plasma sHLA-G level (≥median, 32.0 U/ml) had a significantly shorter survival time (P= 0.044); however, no similar significance was observed for the lesion HLA-G expression.In vitro data showed that both cell surface HLA-G and patient plasma sHLA-G could dramatically decrease the NK cell cytolysis.
Affiliation: Human Tissue Bank, Taizhou Hospital of Zhejiang Province, Wenzhou Medical College, Linhai, Zhejiang, China.Show MeSH
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Mentions: Overall, 41.6% (42/101) tumour lesions were classified as HLA-G+ (Table 1). In the malignant tumour sections, the intensity of staining varied from tumour to tumour and from one area to another within the same tumour. Heterogeneous staining was noted in all histological types of NSCLC. Some tumours showed focal patchy positive staining, and others displayed uniform staining pattern in tumour nests. Positive staining was observed in both the cell membrane and the sub-membranous cytoplasm region. The cytotrophoblasts were used as internal positive control for HLA-G expression. No staining was detected in corresponding adjacent normal lung tissue and tissue sections incubated with irrelevant mouse IgG1 (Fig. 1A).
Affiliation: Human Tissue Bank, Taizhou Hospital of Zhejiang Province, Wenzhou Medical College, Linhai, Zhejiang, China.