Limits...
Selective CB2 up-regulation in women affected by endometrial inflammation.

Iuvone T, De Filippis D, Di Spiezio Sardo A, D'Amico A, Simonetti S, Sparice S, Esposito G, Bifulco G, Insabato L, Nappi C, Guida M - J. Cell. Mol. Med. (2008)

Bottom Line: As a consequence of nitric oxide-dependent injury, the cells respond by triggering protective mechanisms, by changing the endocannabinoid system (ECS) which comprises both CB(1) and CB(2) cannabinoid receptors and their endogenous ligands.In addition, we demonstrated an increase in chymase expression, a marker of mast cells, in biopsies of women affected by endometritis.Therefore our results support the hypothesis that the up-regulation of CB(2) occurs mainly on mast cells and that it might tend to sensitize these cells to the anti-inflammatory effect exerted by endogenous cannabinoids by binding their receptor and thus preventing the mast cell degranulation and the release of pro-inflammatory mediators.

View Article: PubMed Central - PubMed

Affiliation: Endocannabinoid Research Group, Department of Experimental Pharmacology, Faculty of Pharmacy, University of Naples, Federico II, Naples, Italy. iuvone@unina.it

ABSTRACT
Endometritis is defined as an inflammation of the endometrial mucosa of the uterus. In endometritis large amounts of toxic mediators, including nitric oxide (NO) are released by inflammatory cells. As a consequence of nitric oxide-dependent injury, the cells respond by triggering protective mechanisms, by changing the endocannabinoid system (ECS) which comprises both CB(1) and CB(2) cannabinoid receptors and their endogenous ligands. The aim of our study was to seek out evidence for the presence of cannabinoid receptors in inflammatory endometrial tissue as well as for their potential role in endometrial inflammation. Our results showed a selective up-regulation of both transcription and expression of CB(2) receptors in biopsies from women affected by endometrial inflammation compared to healthy women. The experiments with the nitric oxide-donor S-Nitroso-L-Glutathione (GSNO) suggest that such a selective up-regulation may be related to the nitric oxide release occurring during endometrial inflammation. In addition, we demonstrated an increase in chymase expression, a marker of mast cells, in biopsies of women affected by endometritis. Therefore our results support the hypothesis that the up-regulation of CB(2) occurs mainly on mast cells and that it might tend to sensitize these cells to the anti-inflammatory effect exerted by endogenous cannabinoids by binding their receptor and thus preventing the mast cell degranulation and the release of pro-inflammatory mediators. In conclusion, we believe that the selective CB(2) up-regulation might play a role as a novel prognostic factor in endometrial inflammation.

Show MeSH

Related in: MedlinePlus

Representative Western blot analysis (A) and relative densitometric analysis (B) of chymase (MW 45 kD), a marker of mast cells, in biopsies from healthy women and women suffering of endometritis. Tubulin expression is shown as control. Data are representative of six separated experiments. Each bar in the graph shows the mean ±SEM of six experiments. ***P<0.001 versus healthy patients.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3822551&req=5

fig02: Representative Western blot analysis (A) and relative densitometric analysis (B) of chymase (MW 45 kD), a marker of mast cells, in biopsies from healthy women and women suffering of endometritis. Tubulin expression is shown as control. Data are representative of six separated experiments. Each bar in the graph shows the mean ±SEM of six experiments. ***P<0.001 versus healthy patients.

Mentions: Western blot analysis of biopsies obtained from patients affected by endometritis revealed an increase in chymase expression, a specific preformed mediator released by MCs, compared to healthy patients (Fig. 2), thus confirming the presence of MCs in the inflammatory endometrial tissue.


Selective CB2 up-regulation in women affected by endometrial inflammation.

Iuvone T, De Filippis D, Di Spiezio Sardo A, D'Amico A, Simonetti S, Sparice S, Esposito G, Bifulco G, Insabato L, Nappi C, Guida M - J. Cell. Mol. Med. (2008)

Representative Western blot analysis (A) and relative densitometric analysis (B) of chymase (MW 45 kD), a marker of mast cells, in biopsies from healthy women and women suffering of endometritis. Tubulin expression is shown as control. Data are representative of six separated experiments. Each bar in the graph shows the mean ±SEM of six experiments. ***P<0.001 versus healthy patients.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822551&req=5

fig02: Representative Western blot analysis (A) and relative densitometric analysis (B) of chymase (MW 45 kD), a marker of mast cells, in biopsies from healthy women and women suffering of endometritis. Tubulin expression is shown as control. Data are representative of six separated experiments. Each bar in the graph shows the mean ±SEM of six experiments. ***P<0.001 versus healthy patients.
Mentions: Western blot analysis of biopsies obtained from patients affected by endometritis revealed an increase in chymase expression, a specific preformed mediator released by MCs, compared to healthy patients (Fig. 2), thus confirming the presence of MCs in the inflammatory endometrial tissue.

Bottom Line: As a consequence of nitric oxide-dependent injury, the cells respond by triggering protective mechanisms, by changing the endocannabinoid system (ECS) which comprises both CB(1) and CB(2) cannabinoid receptors and their endogenous ligands.In addition, we demonstrated an increase in chymase expression, a marker of mast cells, in biopsies of women affected by endometritis.Therefore our results support the hypothesis that the up-regulation of CB(2) occurs mainly on mast cells and that it might tend to sensitize these cells to the anti-inflammatory effect exerted by endogenous cannabinoids by binding their receptor and thus preventing the mast cell degranulation and the release of pro-inflammatory mediators.

View Article: PubMed Central - PubMed

Affiliation: Endocannabinoid Research Group, Department of Experimental Pharmacology, Faculty of Pharmacy, University of Naples, Federico II, Naples, Italy. iuvone@unina.it

ABSTRACT
Endometritis is defined as an inflammation of the endometrial mucosa of the uterus. In endometritis large amounts of toxic mediators, including nitric oxide (NO) are released by inflammatory cells. As a consequence of nitric oxide-dependent injury, the cells respond by triggering protective mechanisms, by changing the endocannabinoid system (ECS) which comprises both CB(1) and CB(2) cannabinoid receptors and their endogenous ligands. The aim of our study was to seek out evidence for the presence of cannabinoid receptors in inflammatory endometrial tissue as well as for their potential role in endometrial inflammation. Our results showed a selective up-regulation of both transcription and expression of CB(2) receptors in biopsies from women affected by endometrial inflammation compared to healthy women. The experiments with the nitric oxide-donor S-Nitroso-L-Glutathione (GSNO) suggest that such a selective up-regulation may be related to the nitric oxide release occurring during endometrial inflammation. In addition, we demonstrated an increase in chymase expression, a marker of mast cells, in biopsies of women affected by endometritis. Therefore our results support the hypothesis that the up-regulation of CB(2) occurs mainly on mast cells and that it might tend to sensitize these cells to the anti-inflammatory effect exerted by endogenous cannabinoids by binding their receptor and thus preventing the mast cell degranulation and the release of pro-inflammatory mediators. In conclusion, we believe that the selective CB(2) up-regulation might play a role as a novel prognostic factor in endometrial inflammation.

Show MeSH
Related in: MedlinePlus