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VEGF concentration from plasma-activated platelets rich correlates with microvascular density and grading in canine mast cell tumour spontaneous model.

Patruno R, Arpaia N, Gadaleta CD, Passantino L, Zizzo N, Misino A, Lucarelli NM, Catino A, Valerio P, Ribatti D, Ranieri G - J. Cell. Mol. Med. (2008)

Bottom Line: CMCT is classified in three subgroups, well and intermediately differentiated (G1 and G2), corresponding to a benign disease, and poorly differentiated (G3), corresponding to a malignant disease, which metastasize to lymph nodes, liver, spleen and bone marrow.Results show that VEGF level from cytosol P-APR and MVD were significantly higher in G3 CMCTs as compared to G1 or G2 subgroups.Moreover, a significantly strong correlation among VEGF levels from P-PAR and cytosol, MVD and MCD was found in G3 subgroup.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Health and Well-Being, University of Bari Veterinary Medical School, Bari, Italy.

ABSTRACT
Canine cutaneous mast cell tumour (CMCT) is a common cutaneous tumour in dog, with a higher incidence than in human. CMCT is classified in three subgroups, well and intermediately differentiated (G1 and G2), corresponding to a benign disease, and poorly differentiated (G3), corresponding to a malignant disease, which metastasize to lymph nodes, liver, spleen and bone marrow. In this study, we have evaluated serum (S), platelet-poor plasma (P-PP), plasma-activated platelet rich (P-APR) and cytosol vascular endothelial growth factor (VEGF) concentrations, microvascular density (MVD) and mast cell density (MCD) in a series of 86 CMCTs and we have correlated these parameters with each other, by means of ELISA detection of VEGF and immunohistochemistry. Results show that VEGF level from cytosol P-APR and MVD were significantly higher in G3 CMCTs as compared to G1 or G2 subgroups. Moreover, a significantly strong correlation among VEGF levels from P-PAR and cytosol, MVD and MCD was found in G3 subgroup. Because VEGF levels from P-APR well correlated with MVD and malignancy grade in CMCT, we suggest that VEGF might be secreted from MCs and it may be a suitable surrogate inter-species angiogenetic markers of tumour progression in CMCT. Finally, CMCT seems to be a useful model to study the role of MCs in tumour angiogenesis and inhibition of MCs degranulation or activation might be a new anti-angiogenic strategy worthy to further investigations.

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Related in: MedlinePlus

Correlation analysis in highly vascularized poorly differentiated (G3) CMCT subgroup between VEGF concentrations from P-APR and VEGF from cytosol (r = 0.83, P= 0.001); VEGF concentrations from P-APR and MVD (r = 0.82, P= 0.001); VEGF concentrations from P-APR and MCD (r = 0.76, P= 0.001); VEGF concentrations from cytosol and MVD (r = 0.71, P= 0.002); VEGF concentrations from cytosol and MCD (r = 0.69, P= 0.003) MVD and MCD (r = 0.71, P= 0.002).
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fig04: Correlation analysis in highly vascularized poorly differentiated (G3) CMCT subgroup between VEGF concentrations from P-APR and VEGF from cytosol (r = 0.83, P= 0.001); VEGF concentrations from P-APR and MVD (r = 0.82, P= 0.001); VEGF concentrations from P-APR and MCD (r = 0.76, P= 0.001); VEGF concentrations from cytosol and MVD (r = 0.71, P= 0.002); VEGF concentrations from cytosol and MCD (r = 0.69, P= 0.003) MVD and MCD (r = 0.71, P= 0.002).

Mentions: A significantly correlation has been established between these parameters: circulating VEGF from P-APR and VEGF from cytosol (r = 0.83, P= 0.001); circulating VEGF from P-APR and MVD (r = 0.82, P= 0.001); circulating VEGF from P-APR and MCD (r = 0.76, P= 0.001); VEGF from cytosol and MVD (r = 0.71, P= 0.002); VEGF from cytosol and MCD (r = 0.69, P= 0.003); and MVD and MCD (r = 0.71, P= 0.002), only in G3 CMCT subgroup (Fig. 4).


VEGF concentration from plasma-activated platelets rich correlates with microvascular density and grading in canine mast cell tumour spontaneous model.

Patruno R, Arpaia N, Gadaleta CD, Passantino L, Zizzo N, Misino A, Lucarelli NM, Catino A, Valerio P, Ribatti D, Ranieri G - J. Cell. Mol. Med. (2008)

Correlation analysis in highly vascularized poorly differentiated (G3) CMCT subgroup between VEGF concentrations from P-APR and VEGF from cytosol (r = 0.83, P= 0.001); VEGF concentrations from P-APR and MVD (r = 0.82, P= 0.001); VEGF concentrations from P-APR and MCD (r = 0.76, P= 0.001); VEGF concentrations from cytosol and MVD (r = 0.71, P= 0.002); VEGF concentrations from cytosol and MCD (r = 0.69, P= 0.003) MVD and MCD (r = 0.71, P= 0.002).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822515&req=5

fig04: Correlation analysis in highly vascularized poorly differentiated (G3) CMCT subgroup between VEGF concentrations from P-APR and VEGF from cytosol (r = 0.83, P= 0.001); VEGF concentrations from P-APR and MVD (r = 0.82, P= 0.001); VEGF concentrations from P-APR and MCD (r = 0.76, P= 0.001); VEGF concentrations from cytosol and MVD (r = 0.71, P= 0.002); VEGF concentrations from cytosol and MCD (r = 0.69, P= 0.003) MVD and MCD (r = 0.71, P= 0.002).
Mentions: A significantly correlation has been established between these parameters: circulating VEGF from P-APR and VEGF from cytosol (r = 0.83, P= 0.001); circulating VEGF from P-APR and MVD (r = 0.82, P= 0.001); circulating VEGF from P-APR and MCD (r = 0.76, P= 0.001); VEGF from cytosol and MVD (r = 0.71, P= 0.002); VEGF from cytosol and MCD (r = 0.69, P= 0.003); and MVD and MCD (r = 0.71, P= 0.002), only in G3 CMCT subgroup (Fig. 4).

Bottom Line: CMCT is classified in three subgroups, well and intermediately differentiated (G1 and G2), corresponding to a benign disease, and poorly differentiated (G3), corresponding to a malignant disease, which metastasize to lymph nodes, liver, spleen and bone marrow.Results show that VEGF level from cytosol P-APR and MVD were significantly higher in G3 CMCTs as compared to G1 or G2 subgroups.Moreover, a significantly strong correlation among VEGF levels from P-PAR and cytosol, MVD and MCD was found in G3 subgroup.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Health and Well-Being, University of Bari Veterinary Medical School, Bari, Italy.

ABSTRACT
Canine cutaneous mast cell tumour (CMCT) is a common cutaneous tumour in dog, with a higher incidence than in human. CMCT is classified in three subgroups, well and intermediately differentiated (G1 and G2), corresponding to a benign disease, and poorly differentiated (G3), corresponding to a malignant disease, which metastasize to lymph nodes, liver, spleen and bone marrow. In this study, we have evaluated serum (S), platelet-poor plasma (P-PP), plasma-activated platelet rich (P-APR) and cytosol vascular endothelial growth factor (VEGF) concentrations, microvascular density (MVD) and mast cell density (MCD) in a series of 86 CMCTs and we have correlated these parameters with each other, by means of ELISA detection of VEGF and immunohistochemistry. Results show that VEGF level from cytosol P-APR and MVD were significantly higher in G3 CMCTs as compared to G1 or G2 subgroups. Moreover, a significantly strong correlation among VEGF levels from P-PAR and cytosol, MVD and MCD was found in G3 subgroup. Because VEGF levels from P-APR well correlated with MVD and malignancy grade in CMCT, we suggest that VEGF might be secreted from MCs and it may be a suitable surrogate inter-species angiogenetic markers of tumour progression in CMCT. Finally, CMCT seems to be a useful model to study the role of MCs in tumour angiogenesis and inhibition of MCs degranulation or activation might be a new anti-angiogenic strategy worthy to further investigations.

Show MeSH
Related in: MedlinePlus