FHL1 interacts with oestrogen receptors and regulates breast cancer cell growth.
Bottom Line: Here, we show that FHL1 physically and functionally interacted with oestrogen receptors (ERs), which are involved in breast cancer development and progression.Further analysis of 46 breast cancer samples showed that FHL1 expression negatively associated with oestrogen-responsive gene expression in breast cancer cells.These results suggest that FHL1 may play an important role in ER signalling as well as breast cancer cell growth regulation.
Affiliation: Department of Molecular Oncology, Beijing Institute of Biotechnology, Beijing, China.Show MeSH
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Mentions: To test the effect of FHL1 on breast cancer cell growth, the anchorage-dependent growth rate of ZR75–1 cells transfected with FHL1 or empty vector, or with FHL1 siRNAs or control siRNA, was examined. In the presence or absence of E2, ZR75–1 cells transfected with FHL1 grew more slowly than those with empty vector (Fig. 6A), whereas ZR75–1 cells transfected with FHL1 siRNAs grew faster than those with control siRNA (Fig. 6B). Similar results were observed in MCF7 cells (data not shown). In addition, in agreement with the results of the transcriptional experiments, the anti-oestrogens 4-OHT and ICI 182,780 further promoted the effect of FHL1 on breast cancer cell growth (Fig. 6C).
Affiliation: Department of Molecular Oncology, Beijing Institute of Biotechnology, Beijing, China.