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Bone marrow-derived cells can acquire cardiac stem cells properties in damaged heart.

Barile L, Cerisoli F, Frati G, Gaetani R, Chimenti I, Forte E, Cassinelli L, Spinardi L, Altomare C, Kizana E, Giacomello A, Messina E, Ottolenghi S, Magli MC - J. Cell. Mol. Med. (2011)

Bottom Line: However, there is no direct evidence, so far, that BM cells can generate cardiac stem cells (CSCs).Following haematological reconstitution and MI, CSCs were cultured from cardiac explants to generate 'cardiospheres', a microtissue normally originating in vitro from CSCs.These were all green fluorescent (i.e. BM derived) and contained cells capable of initiating differentiation into cells expressing the cardiac marker Nkx2.5.

View Article: PubMed Central - PubMed

Affiliation: Institut Pasteur-Cenci Bolognetti Foundation, Department of Experimental Medicine, University of Rome La Sapienza, Rome, Italy.

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Kit/GFP+ CS and CS-forming-cells. (A) Kit/GFP+ CS-forming cells were collected from the explant of infarcted heart 13 days after the beginning of the culture, and plated onto poly-D-lysine for 1 day to allow CS formation. Note the initial appearance of slightly fluorescent cells. (B) A CS at a more advanced stage of development shows transgenic Kit/GFP expressing cells at the centre of the sphere. (C) PCR analysis of Kit/GFP in genomic DNA extracted from single CSs. 351–441 indicate different transplanted-infarcted mice; LD, sizemarker; C1a and C1b represent internal positive controls (genomic DNA from 107 and 106Kit/GFP BM cells); WT, genomic DNA from 107 wild-type BM cells; PCR-, negative control on PCR mix. Product of amplification from sample 438 was confirmed by in vitro automatic sequencing (not shown).
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fig02: Kit/GFP+ CS and CS-forming-cells. (A) Kit/GFP+ CS-forming cells were collected from the explant of infarcted heart 13 days after the beginning of the culture, and plated onto poly-D-lysine for 1 day to allow CS formation. Note the initial appearance of slightly fluorescent cells. (B) A CS at a more advanced stage of development shows transgenic Kit/GFP expressing cells at the centre of the sphere. (C) PCR analysis of Kit/GFP in genomic DNA extracted from single CSs. 351–441 indicate different transplanted-infarcted mice; LD, sizemarker; C1a and C1b represent internal positive controls (genomic DNA from 107 and 106Kit/GFP BM cells); WT, genomic DNA from 107 wild-type BM cells; PCR-, negative control on PCR mix. Product of amplification from sample 438 was confirmed by in vitro automatic sequencing (not shown).

Mentions: In parallel, myocardial tissues were cultured as explants and after 1–4 weeks an adherent layer of fibroblast-like cells was formed and small, phase-bright cells migrated on top of it. These phase-bright cells were plated under conditions supporting the formation of CSs (Fig. 2). CS-forming cells were harvested at least four times at 6- to 10-day intervals from the same explant. Once formed, primary CSs (5–10/heart) were collected and amplified [1, 8], by growing them as adherent cells for 1 month, and subsequently transferred back onto poly-D-lysine-coated plates to generate large numbers of secondary CSs for analysis.


Bone marrow-derived cells can acquire cardiac stem cells properties in damaged heart.

Barile L, Cerisoli F, Frati G, Gaetani R, Chimenti I, Forte E, Cassinelli L, Spinardi L, Altomare C, Kizana E, Giacomello A, Messina E, Ottolenghi S, Magli MC - J. Cell. Mol. Med. (2011)

Kit/GFP+ CS and CS-forming-cells. (A) Kit/GFP+ CS-forming cells were collected from the explant of infarcted heart 13 days after the beginning of the culture, and plated onto poly-D-lysine for 1 day to allow CS formation. Note the initial appearance of slightly fluorescent cells. (B) A CS at a more advanced stage of development shows transgenic Kit/GFP expressing cells at the centre of the sphere. (C) PCR analysis of Kit/GFP in genomic DNA extracted from single CSs. 351–441 indicate different transplanted-infarcted mice; LD, sizemarker; C1a and C1b represent internal positive controls (genomic DNA from 107 and 106Kit/GFP BM cells); WT, genomic DNA from 107 wild-type BM cells; PCR-, negative control on PCR mix. Product of amplification from sample 438 was confirmed by in vitro automatic sequencing (not shown).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3822494&req=5

fig02: Kit/GFP+ CS and CS-forming-cells. (A) Kit/GFP+ CS-forming cells were collected from the explant of infarcted heart 13 days after the beginning of the culture, and plated onto poly-D-lysine for 1 day to allow CS formation. Note the initial appearance of slightly fluorescent cells. (B) A CS at a more advanced stage of development shows transgenic Kit/GFP expressing cells at the centre of the sphere. (C) PCR analysis of Kit/GFP in genomic DNA extracted from single CSs. 351–441 indicate different transplanted-infarcted mice; LD, sizemarker; C1a and C1b represent internal positive controls (genomic DNA from 107 and 106Kit/GFP BM cells); WT, genomic DNA from 107 wild-type BM cells; PCR-, negative control on PCR mix. Product of amplification from sample 438 was confirmed by in vitro automatic sequencing (not shown).
Mentions: In parallel, myocardial tissues were cultured as explants and after 1–4 weeks an adherent layer of fibroblast-like cells was formed and small, phase-bright cells migrated on top of it. These phase-bright cells were plated under conditions supporting the formation of CSs (Fig. 2). CS-forming cells were harvested at least four times at 6- to 10-day intervals from the same explant. Once formed, primary CSs (5–10/heart) were collected and amplified [1, 8], by growing them as adherent cells for 1 month, and subsequently transferred back onto poly-D-lysine-coated plates to generate large numbers of secondary CSs for analysis.

Bottom Line: However, there is no direct evidence, so far, that BM cells can generate cardiac stem cells (CSCs).Following haematological reconstitution and MI, CSCs were cultured from cardiac explants to generate 'cardiospheres', a microtissue normally originating in vitro from CSCs.These were all green fluorescent (i.e. BM derived) and contained cells capable of initiating differentiation into cells expressing the cardiac marker Nkx2.5.

View Article: PubMed Central - PubMed

Affiliation: Institut Pasteur-Cenci Bolognetti Foundation, Department of Experimental Medicine, University of Rome La Sapienza, Rome, Italy.

Show MeSH
Related in: MedlinePlus