Bone marrow-derived cells can acquire cardiac stem cells properties in damaged heart.
Bottom Line: However, there is no direct evidence, so far, that BM cells can generate cardiac stem cells (CSCs).Following haematological reconstitution and MI, CSCs were cultured from cardiac explants to generate 'cardiospheres', a microtissue normally originating in vitro from CSCs.These were all green fluorescent (i.e. BM derived) and contained cells capable of initiating differentiation into cells expressing the cardiac marker Nkx2.5.
Affiliation: Institut Pasteur-Cenci Bolognetti Foundation, Department of Experimental Medicine, University of Rome La Sapienza, Rome, Italy.Show MeSH
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Mentions: In parallel, myocardial tissues were cultured as explants and after 1–4 weeks an adherent layer of fibroblast-like cells was formed and small, phase-bright cells migrated on top of it. These phase-bright cells were plated under conditions supporting the formation of CSs (Fig. 2). CS-forming cells were harvested at least four times at 6- to 10-day intervals from the same explant. Once formed, primary CSs (5–10/heart) were collected and amplified [1, 8], by growing them as adherent cells for 1 month, and subsequently transferred back onto poly-D-lysine-coated plates to generate large numbers of secondary CSs for analysis.
Affiliation: Institut Pasteur-Cenci Bolognetti Foundation, Department of Experimental Medicine, University of Rome La Sapienza, Rome, Italy.