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Stem cell-mediated natural tissue engineering.

Möllmann H, Nef HM, Voss S, Troidl C, Willmer M, Szardien S, Rolf A, Klement M, Voswinckel R, Kostin S, Ghofrani HA, Hamm CW, Elsässer A - J. Cell. Mol. Med. (2011)

Bottom Line: The GM-CSF effect on cell proliferation outlasted the treat ment period by several weeks.Circulating stem cells contributed to the development of a fully differentiated tissue on membranes placed within the left ventricle or descending aorta under physiological conditions.Early cardiomyocyte generation was identified only on membranes positioned within the left ventricle.

View Article: PubMed Central - PubMed

Affiliation: Kerckhoff Heart Center, Department of Cardiology, Bad Nauheim, Germany. h.moellmann@kerckhoff-fgi.de

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Related in: MedlinePlus

(A) Fibroblasts are stained with vimentin (red), smooth muscle cells are stained with a-smooth muscle actin (α-SMA, green). Colocalization of vimentin and α-SMA is typical for myofibroblasts, which appear yellow (arrows). Nuclei are blue (#: implanted membrane). (B) Myofibroblasts specifically stained with anti-non-muscle myosin heavy chain b (SMemb; green); nuclei are blue. (C) Cells expressing myosin heavy chain were stained with MF20 (arrows, green), nuclei are red (#: implanted membrane). (D) Mef2c, a marker of muscle cells, was detected in aortic (Ao) and left ventricular membranes (qRT-PCR; n = 8 per time-point). (E) Electron microscopy image showing clear orientation of cells in the newly developed tissue after 12 weeks. (F) Electron microscopy image showing different types of differentiated cells after 6 weeks (SMC, smooth muscle cell; Fb, fibroblast; rER, rough endoplasmic reticulum). (G) Electron microscopy image showing SMCs and organized collagen fibres (coll). Intracellular dense bodies (arrowhead) indicate terminal differentiation and contractile function of SMCs. Focal adhesion points (arrow) hallmark cellular interaction with the extracellular matrix, implying functional organization.
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fig03: (A) Fibroblasts are stained with vimentin (red), smooth muscle cells are stained with a-smooth muscle actin (α-SMA, green). Colocalization of vimentin and α-SMA is typical for myofibroblasts, which appear yellow (arrows). Nuclei are blue (#: implanted membrane). (B) Myofibroblasts specifically stained with anti-non-muscle myosin heavy chain b (SMemb; green); nuclei are blue. (C) Cells expressing myosin heavy chain were stained with MF20 (arrows, green), nuclei are red (#: implanted membrane). (D) Mef2c, a marker of muscle cells, was detected in aortic (Ao) and left ventricular membranes (qRT-PCR; n = 8 per time-point). (E) Electron microscopy image showing clear orientation of cells in the newly developed tissue after 12 weeks. (F) Electron microscopy image showing different types of differentiated cells after 6 weeks (SMC, smooth muscle cell; Fb, fibroblast; rER, rough endoplasmic reticulum). (G) Electron microscopy image showing SMCs and organized collagen fibres (coll). Intracellular dense bodies (arrowhead) indicate terminal differentiation and contractile function of SMCs. Focal adhesion points (arrow) hallmark cellular interaction with the extracellular matrix, implying functional organization.

Mentions: The inner part of the newly developed tissue contained large numbers of α-smooth muscle actin (α-SMA)+ cells. Several of these cells were myofibroblasts, given the colocalization of α-SMA with vimentin (Fig. 3A) and their positive staining for embryonic smooth muscle myosin heavy chain (SMemb; Fig. 3B). The growing tissue was mainly defined by fibroblasts, as characterized by their typical shape and positive vimentin staining.


Stem cell-mediated natural tissue engineering.

Möllmann H, Nef HM, Voss S, Troidl C, Willmer M, Szardien S, Rolf A, Klement M, Voswinckel R, Kostin S, Ghofrani HA, Hamm CW, Elsässer A - J. Cell. Mol. Med. (2011)

(A) Fibroblasts are stained with vimentin (red), smooth muscle cells are stained with a-smooth muscle actin (α-SMA, green). Colocalization of vimentin and α-SMA is typical for myofibroblasts, which appear yellow (arrows). Nuclei are blue (#: implanted membrane). (B) Myofibroblasts specifically stained with anti-non-muscle myosin heavy chain b (SMemb; green); nuclei are blue. (C) Cells expressing myosin heavy chain were stained with MF20 (arrows, green), nuclei are red (#: implanted membrane). (D) Mef2c, a marker of muscle cells, was detected in aortic (Ao) and left ventricular membranes (qRT-PCR; n = 8 per time-point). (E) Electron microscopy image showing clear orientation of cells in the newly developed tissue after 12 weeks. (F) Electron microscopy image showing different types of differentiated cells after 6 weeks (SMC, smooth muscle cell; Fb, fibroblast; rER, rough endoplasmic reticulum). (G) Electron microscopy image showing SMCs and organized collagen fibres (coll). Intracellular dense bodies (arrowhead) indicate terminal differentiation and contractile function of SMCs. Focal adhesion points (arrow) hallmark cellular interaction with the extracellular matrix, implying functional organization.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822493&req=5

fig03: (A) Fibroblasts are stained with vimentin (red), smooth muscle cells are stained with a-smooth muscle actin (α-SMA, green). Colocalization of vimentin and α-SMA is typical for myofibroblasts, which appear yellow (arrows). Nuclei are blue (#: implanted membrane). (B) Myofibroblasts specifically stained with anti-non-muscle myosin heavy chain b (SMemb; green); nuclei are blue. (C) Cells expressing myosin heavy chain were stained with MF20 (arrows, green), nuclei are red (#: implanted membrane). (D) Mef2c, a marker of muscle cells, was detected in aortic (Ao) and left ventricular membranes (qRT-PCR; n = 8 per time-point). (E) Electron microscopy image showing clear orientation of cells in the newly developed tissue after 12 weeks. (F) Electron microscopy image showing different types of differentiated cells after 6 weeks (SMC, smooth muscle cell; Fb, fibroblast; rER, rough endoplasmic reticulum). (G) Electron microscopy image showing SMCs and organized collagen fibres (coll). Intracellular dense bodies (arrowhead) indicate terminal differentiation and contractile function of SMCs. Focal adhesion points (arrow) hallmark cellular interaction with the extracellular matrix, implying functional organization.
Mentions: The inner part of the newly developed tissue contained large numbers of α-smooth muscle actin (α-SMA)+ cells. Several of these cells were myofibroblasts, given the colocalization of α-SMA with vimentin (Fig. 3A) and their positive staining for embryonic smooth muscle myosin heavy chain (SMemb; Fig. 3B). The growing tissue was mainly defined by fibroblasts, as characterized by their typical shape and positive vimentin staining.

Bottom Line: The GM-CSF effect on cell proliferation outlasted the treat ment period by several weeks.Circulating stem cells contributed to the development of a fully differentiated tissue on membranes placed within the left ventricle or descending aorta under physiological conditions.Early cardiomyocyte generation was identified only on membranes positioned within the left ventricle.

View Article: PubMed Central - PubMed

Affiliation: Kerckhoff Heart Center, Department of Cardiology, Bad Nauheim, Germany. h.moellmann@kerckhoff-fgi.de

Show MeSH
Related in: MedlinePlus