Stem cell-mediated natural tissue engineering.
Bottom Line: The GM-CSF effect on cell proliferation outlasted the treat ment period by several weeks.Circulating stem cells contributed to the development of a fully differentiated tissue on membranes placed within the left ventricle or descending aorta under physiological conditions.Early cardiomyocyte generation was identified only on membranes positioned within the left ventricle.
Affiliation: Kerckhoff Heart Center, Department of Cardiology, Bad Nauheim, Germany. firstname.lastname@example.orgShow MeSH
Related in: MedlinePlus
Mentions: The inner part of the newly developed tissue contained large numbers of α-smooth muscle actin (α-SMA)+ cells. Several of these cells were myofibroblasts, given the colocalization of α-SMA with vimentin (Fig. 3A) and their positive staining for embryonic smooth muscle myosin heavy chain (SMemb; Fig. 3B). The growing tissue was mainly defined by fibroblasts, as characterized by their typical shape and positive vimentin staining.
Affiliation: Kerckhoff Heart Center, Department of Cardiology, Bad Nauheim, Germany. email@example.com