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Life in Science: Armin Dale Kaiser.

Kaiser AD - Bacteriophage (2013)

View Article: PubMed Central - PubMed

Affiliation: Department of Developmental Biology; Stanford School of Medicine; Stanford, CA USA.

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Pauling, then chairman of chemistry at Caltech, and working very much in the scientific spirit of Morgan, was making very important contributions to molecular biology by working out the structure of proteins... He predicted that the left-handed α helix would be found in hair and horn... He also said it was likely to be found in the backbone of some globular proteins like lysozyme, which indeed it is... This was the origin of the Visconti-Delbruck theory of phage genetics, which Delbruck worked out in quantitative detail... Delbruck had invited me to accompany him to Cold Spring Harbor that summer, and it was exciting to see the complex body of Visconti’s data fall into line as the logical conclusions of a number of clearly stated and plausible assumptions... Weigle investigated the induction of bacteriophage lambda by UV light, and he found many plaque morphology mutants in his irradiated cultures... While working on induction of lambda by UV light, he discovered reactivation of UV-killed lambda by UV irradiating the bacteria on which the phage had been plated... A surprising result then, it proved to be one of the first observations of the bacterial SOS response... My thesis research consisted of a series of two and three-factor crosses involving 5 mutant alleles of bacteriophage lambda that arose in Weigle’s experiments... From cultures of E. coli, acquired from Paris hospitals, Jacob and Wollman had isolated a series of different temperate bacteriophages that were serologically related to lambda and that could recombine with it, but that had different prophage immunities... The visit gave me the opportunity to learn the application of Caspar and Klug’s theory of quasi-equivalence for the symmetry of moderately large, spherical DNA viruses, like bacteriophage λ with icosahedral symmetry but more than 60 subunits... Using concentrated soluble extracts of E. coli cells that were lysogenic for λ, we added pure, genetically marked λ DNA and observed the formation of morphologically complete and infective phage particles... This experiment showed that the cutting and the packaging of DNA inside a head is coupled to the assembly of certain head protein subunits... In vitro packaging of λ DNA was significant, not only because it was the first DNA to be packaged in vitro, but also because it allowed each step in the packaging process to be investigated.

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Armin Dale Kaiser, Professor of Biochemistry and of Developmental Biology, Emeritus, Stanford University Medical School
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Figure 1: Armin Dale Kaiser, Professor of Biochemistry and of Developmental Biology, Emeritus, Stanford University Medical School


Life in Science: Armin Dale Kaiser.

Kaiser AD - Bacteriophage (2013)

Armin Dale Kaiser, Professor of Biochemistry and of Developmental Biology, Emeritus, Stanford University Medical School
© Copyright Policy - open-access
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC3821691&req=5

Figure 1: Armin Dale Kaiser, Professor of Biochemistry and of Developmental Biology, Emeritus, Stanford University Medical School

View Article: PubMed Central - PubMed

Affiliation: Department of Developmental Biology; Stanford School of Medicine; Stanford, CA USA.

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Pauling, then chairman of chemistry at Caltech, and working very much in the scientific spirit of Morgan, was making very important contributions to molecular biology by working out the structure of proteins... He predicted that the left-handed α helix would be found in hair and horn... He also said it was likely to be found in the backbone of some globular proteins like lysozyme, which indeed it is... This was the origin of the Visconti-Delbruck theory of phage genetics, which Delbruck worked out in quantitative detail... Delbruck had invited me to accompany him to Cold Spring Harbor that summer, and it was exciting to see the complex body of Visconti’s data fall into line as the logical conclusions of a number of clearly stated and plausible assumptions... Weigle investigated the induction of bacteriophage lambda by UV light, and he found many plaque morphology mutants in his irradiated cultures... While working on induction of lambda by UV light, he discovered reactivation of UV-killed lambda by UV irradiating the bacteria on which the phage had been plated... A surprising result then, it proved to be one of the first observations of the bacterial SOS response... My thesis research consisted of a series of two and three-factor crosses involving 5 mutant alleles of bacteriophage lambda that arose in Weigle’s experiments... From cultures of E. coli, acquired from Paris hospitals, Jacob and Wollman had isolated a series of different temperate bacteriophages that were serologically related to lambda and that could recombine with it, but that had different prophage immunities... The visit gave me the opportunity to learn the application of Caspar and Klug’s theory of quasi-equivalence for the symmetry of moderately large, spherical DNA viruses, like bacteriophage λ with icosahedral symmetry but more than 60 subunits... Using concentrated soluble extracts of E. coli cells that were lysogenic for λ, we added pure, genetically marked λ DNA and observed the formation of morphologically complete and infective phage particles... This experiment showed that the cutting and the packaging of DNA inside a head is coupled to the assembly of certain head protein subunits... In vitro packaging of λ DNA was significant, not only because it was the first DNA to be packaged in vitro, but also because it allowed each step in the packaging process to be investigated.

No MeSH data available.