Limits...
Erucin exerts anti-inflammatory properties in murine macrophages and mouse skin: possible mediation through the inhibition of NFκB signaling.

Cho HJ, Lee KW, Park JH - Int J Mol Sci (2013)

Bottom Line: In RAW 264.7 cells, erucin (2.5, 5 μmol/L) inhibited LPS-induced production of nitric oxide and prostaglandin E2.Erucin inhibited LPS-induced degradation of the inhibitor of κBα and translocation of p65 to the nucleus and, subsequently, reduced LPS-induced nuclear factor κB (NFκB) DNA binding activities, as well as the transcriptional activity of NFκB, leading to the decreased expression of NFκB-target genes, including tumor necrosis factor-α, interleukin (IL)-6, IL-1β, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, as well as transcriptional activity of iNOS and COX-2.These results indicate that erucin exerts a potent anti-inflammatory activity by inhibiting the pro-inflammatory enzymes and cytokines, which may be mediated, at least in part, via the inhibition of NFκB signaling.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Nutrition, Hallym University, Chuncheon 200-702, Korea. jyoon@hallym.ac.kr.

ABSTRACT
Erucin, an isothiocyanate, is a hydrolysis product of glucoerucin found in arugula and has recently been reported to have anti-cancer properties in various cancer cells. In this study, we assessed the anti-inflammatory effects of erucin and the underlying mechanisms, using lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophages and 12-O-tetradecanoylphorbol-13-acetate-treated mouse skin. In RAW 264.7 cells, erucin (2.5, 5 μmol/L) inhibited LPS-induced production of nitric oxide and prostaglandin E2. Erucin inhibited LPS-induced degradation of the inhibitor of κBα and translocation of p65 to the nucleus and, subsequently, reduced LPS-induced nuclear factor κB (NFκB) DNA binding activities, as well as the transcriptional activity of NFκB, leading to the decreased expression of NFκB-target genes, including tumor necrosis factor-α, interleukin (IL)-6, IL-1β, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, as well as transcriptional activity of iNOS and COX-2. In mice, erucin (100, 300 nmoles) treatment significantly inhibited phorbol ester-induced formation of ear edema and expression of iNOS and COX-2 proteins. These results indicate that erucin exerts a potent anti-inflammatory activity by inhibiting the pro-inflammatory enzymes and cytokines, which may be mediated, at least in part, via the inhibition of NFκB signaling.

Show MeSH

Related in: MedlinePlus

Erucin decreases lipopolysaccharide (LPS)-induced NO and prostaglandin E2 (PGE2) production in RAW 264.7 cells. (A) Structure of erucin; (B–D) RAW 264.7 cells were treated with various concentrations of erucin in the presence of LPS. Viable cell numbers were estimated by the MTT Assay (B); The 24-h-conditioned media were collected for the estimation of NO (C) and PGE2 (D) concentrations. Each bar represents the mean ± SEM (n = 4). Means without a common letter differ (p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3821631&req=5

f1-ijms-14-20564: Erucin decreases lipopolysaccharide (LPS)-induced NO and prostaglandin E2 (PGE2) production in RAW 264.7 cells. (A) Structure of erucin; (B–D) RAW 264.7 cells were treated with various concentrations of erucin in the presence of LPS. Viable cell numbers were estimated by the MTT Assay (B); The 24-h-conditioned media were collected for the estimation of NO (C) and PGE2 (D) concentrations. Each bar represents the mean ± SEM (n = 4). Means without a common letter differ (p < 0.05).

Mentions: Consumption of cruciferous vegetables is associated with several health benefits against cancer, heart health, diabetes, asthma and Alzheimer’s disease [4,5]. These benefits of cruciferous vegetables have been attributed to their containing various bioactive compounds (sulfur-containing compounds, indole derivatives and phenolic compounds). Among these compounds, isothiocyanates (sulfur-containing compounds and produced by enzymatic conversion of glucosinolates) have been shown to exert various biological effects, including anti-inflammatory properties [6]. For example, benzyl isothiocyanate (BITC), phenethyl isothiocyanate (PITC) and sulforaphane (SFN) have been found to possess anti-inflammatory activities [7–10]. Erucin, 4-(methylthio)butyl isothiocyanate (structure in Figure 1A), is a hydrolysis product of glucoerucin, which is found in arugula, and an in vivo reduction product of SFN. Erucin has been reported to exert chemopreventive effects by various mechanisms, including alteration of phase I, II and III enzymes, regulation of cell proliferation by induction of cell cycle arrest and apoptosis and downregulation of androgen receptor signaling [11].


Erucin exerts anti-inflammatory properties in murine macrophages and mouse skin: possible mediation through the inhibition of NFκB signaling.

Cho HJ, Lee KW, Park JH - Int J Mol Sci (2013)

Erucin decreases lipopolysaccharide (LPS)-induced NO and prostaglandin E2 (PGE2) production in RAW 264.7 cells. (A) Structure of erucin; (B–D) RAW 264.7 cells were treated with various concentrations of erucin in the presence of LPS. Viable cell numbers were estimated by the MTT Assay (B); The 24-h-conditioned media were collected for the estimation of NO (C) and PGE2 (D) concentrations. Each bar represents the mean ± SEM (n = 4). Means without a common letter differ (p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3821631&req=5

f1-ijms-14-20564: Erucin decreases lipopolysaccharide (LPS)-induced NO and prostaglandin E2 (PGE2) production in RAW 264.7 cells. (A) Structure of erucin; (B–D) RAW 264.7 cells were treated with various concentrations of erucin in the presence of LPS. Viable cell numbers were estimated by the MTT Assay (B); The 24-h-conditioned media were collected for the estimation of NO (C) and PGE2 (D) concentrations. Each bar represents the mean ± SEM (n = 4). Means without a common letter differ (p < 0.05).
Mentions: Consumption of cruciferous vegetables is associated with several health benefits against cancer, heart health, diabetes, asthma and Alzheimer’s disease [4,5]. These benefits of cruciferous vegetables have been attributed to their containing various bioactive compounds (sulfur-containing compounds, indole derivatives and phenolic compounds). Among these compounds, isothiocyanates (sulfur-containing compounds and produced by enzymatic conversion of glucosinolates) have been shown to exert various biological effects, including anti-inflammatory properties [6]. For example, benzyl isothiocyanate (BITC), phenethyl isothiocyanate (PITC) and sulforaphane (SFN) have been found to possess anti-inflammatory activities [7–10]. Erucin, 4-(methylthio)butyl isothiocyanate (structure in Figure 1A), is a hydrolysis product of glucoerucin, which is found in arugula, and an in vivo reduction product of SFN. Erucin has been reported to exert chemopreventive effects by various mechanisms, including alteration of phase I, II and III enzymes, regulation of cell proliferation by induction of cell cycle arrest and apoptosis and downregulation of androgen receptor signaling [11].

Bottom Line: In RAW 264.7 cells, erucin (2.5, 5 μmol/L) inhibited LPS-induced production of nitric oxide and prostaglandin E2.Erucin inhibited LPS-induced degradation of the inhibitor of κBα and translocation of p65 to the nucleus and, subsequently, reduced LPS-induced nuclear factor κB (NFκB) DNA binding activities, as well as the transcriptional activity of NFκB, leading to the decreased expression of NFκB-target genes, including tumor necrosis factor-α, interleukin (IL)-6, IL-1β, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, as well as transcriptional activity of iNOS and COX-2.These results indicate that erucin exerts a potent anti-inflammatory activity by inhibiting the pro-inflammatory enzymes and cytokines, which may be mediated, at least in part, via the inhibition of NFκB signaling.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Nutrition, Hallym University, Chuncheon 200-702, Korea. jyoon@hallym.ac.kr.

ABSTRACT
Erucin, an isothiocyanate, is a hydrolysis product of glucoerucin found in arugula and has recently been reported to have anti-cancer properties in various cancer cells. In this study, we assessed the anti-inflammatory effects of erucin and the underlying mechanisms, using lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophages and 12-O-tetradecanoylphorbol-13-acetate-treated mouse skin. In RAW 264.7 cells, erucin (2.5, 5 μmol/L) inhibited LPS-induced production of nitric oxide and prostaglandin E2. Erucin inhibited LPS-induced degradation of the inhibitor of κBα and translocation of p65 to the nucleus and, subsequently, reduced LPS-induced nuclear factor κB (NFκB) DNA binding activities, as well as the transcriptional activity of NFκB, leading to the decreased expression of NFκB-target genes, including tumor necrosis factor-α, interleukin (IL)-6, IL-1β, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, as well as transcriptional activity of iNOS and COX-2. In mice, erucin (100, 300 nmoles) treatment significantly inhibited phorbol ester-induced formation of ear edema and expression of iNOS and COX-2 proteins. These results indicate that erucin exerts a potent anti-inflammatory activity by inhibiting the pro-inflammatory enzymes and cytokines, which may be mediated, at least in part, via the inhibition of NFκB signaling.

Show MeSH
Related in: MedlinePlus