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Combined taurine, epigallocatechin gallate and genistein therapy reduces HSC-T6 cell proliferation and modulates the expression of fibrogenic factors.

Li Y, Luo Y, Zhang X, Lin X, He M, Liao M - Int J Mol Sci (2013)

Bottom Line: Combined drug treatment significantly inhibited cell proliferation and TGF-β1, Col-I, TIMP-1 and TIMP-2 mRNA expression in activated HSC-T6 cells, while the expression of MMP-2 mRNA increased.In conclusion, combined drug treatment with taurine, epigallocatechin gallate and genistein can inhibit HSC proliferation, and impact fibrosis-related gene and protein expression.The antifibrotic effects of this drug combination may be due to its effects on the expression of fibrogenic genes.

View Article: PubMed Central - PubMed

Affiliation: Guangxi University Library, Guangxi University, Nanning 530004, Guangxi, China. lminggx@163.com.

ABSTRACT
Hepatic fibrogenesis involves the activation of hepatic stellate cells (HSCs), which synthesize excess extracellular matrix and contribute to the development of liver fibrosis. In a prior study we tested the effect of combined treatment with taurine, epigallocatechin gallate and genistein on the development of alcohol-induced liver fibrosis in vitro. In this study, the biological activity of the combination of these molecules was assessed by measuring its effect on cell proliferation, fibrosis-related gene expression, and proteomic expression profiling in the activated HSC cell line, HSC-T6. HSC-T6 cells were incubated with different concentrations of the drug combination taurine, epigallocatechin gallate and genistein. Cell proliferation was evaluated by MTT assay. Transforming growth factor β1 (TGF-β1), collagen type I (Col-I), matrix metalloproteinase-2 (MMP-2), and tissue inhibitor of metalloproteinases 1 and 2 (TIMP-1 and TIMP-2) mRNA were analyzed by semi-quantitative reverse-transcription PCR. Proteomic profiling of HSC-T6 cells was also performed by SELDI-TOF-MS. Combined drug treatment significantly inhibited cell proliferation and TGF-β1, Col-I, TIMP-1 and TIMP-2 mRNA expression in activated HSC-T6 cells, while the expression of MMP-2 mRNA increased. A total of 176 protein m/z peaks were identified. The intensities of 10 protein peaks were downregulated and two protein peaks were upregulated in HSC-T6 cells after combined drug treatment. In conclusion, combined drug treatment with taurine, epigallocatechin gallate and genistein can inhibit HSC proliferation, and impact fibrosis-related gene and protein expression. The antifibrotic effects of this drug combination may be due to its effects on the expression of fibrogenic genes.

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Proliferation of HSC-T6 cells (hepatic stellate cells) treated with a combination of taurine, epigallocatechin gallate (EGCG) and genistein. HSC-T6 cell proliferation was measured by MTT assay. The effect of varying doses of taurine, EGCG and genistein were compared to (I) untreated control cells; or cells treated with (II) 0.015 mg/mL taurine, 0.0175 mg/mL EGCG and 0.0035 mg/mL genistein; (III) 0.03 mg/mL taurine, 0.035 mg/mL EGCG and 0.007 mg/mL genistein; or (IV) 0.06 mg/mL taurine, 0.07 mg/mL EGCG and 0.014 mg/mL genistein. Data are presented as the mean ± S.E.; * p <0.05 vs. group I (untreated control).
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f1-ijms-14-20543: Proliferation of HSC-T6 cells (hepatic stellate cells) treated with a combination of taurine, epigallocatechin gallate (EGCG) and genistein. HSC-T6 cell proliferation was measured by MTT assay. The effect of varying doses of taurine, EGCG and genistein were compared to (I) untreated control cells; or cells treated with (II) 0.015 mg/mL taurine, 0.0175 mg/mL EGCG and 0.0035 mg/mL genistein; (III) 0.03 mg/mL taurine, 0.035 mg/mL EGCG and 0.007 mg/mL genistein; or (IV) 0.06 mg/mL taurine, 0.07 mg/mL EGCG and 0.014 mg/mL genistein. Data are presented as the mean ± S.E.; * p <0.05 vs. group I (untreated control).

Mentions: The anti-proliferative effect of combined taurine, EGCG and genistein treatment in HSC-T6 cells was determined by measuring cell viability using MTT assay. After treatment with different doses of the drug combination, the proliferation of all treated groups was significantly reduced compared to control HSCs. As shown in Figure 1, HSC-T6 cell proliferation was remarkably inhibited by the drug combinations.


Combined taurine, epigallocatechin gallate and genistein therapy reduces HSC-T6 cell proliferation and modulates the expression of fibrogenic factors.

Li Y, Luo Y, Zhang X, Lin X, He M, Liao M - Int J Mol Sci (2013)

Proliferation of HSC-T6 cells (hepatic stellate cells) treated with a combination of taurine, epigallocatechin gallate (EGCG) and genistein. HSC-T6 cell proliferation was measured by MTT assay. The effect of varying doses of taurine, EGCG and genistein were compared to (I) untreated control cells; or cells treated with (II) 0.015 mg/mL taurine, 0.0175 mg/mL EGCG and 0.0035 mg/mL genistein; (III) 0.03 mg/mL taurine, 0.035 mg/mL EGCG and 0.007 mg/mL genistein; or (IV) 0.06 mg/mL taurine, 0.07 mg/mL EGCG and 0.014 mg/mL genistein. Data are presented as the mean ± S.E.; * p <0.05 vs. group I (untreated control).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3821629&req=5

f1-ijms-14-20543: Proliferation of HSC-T6 cells (hepatic stellate cells) treated with a combination of taurine, epigallocatechin gallate (EGCG) and genistein. HSC-T6 cell proliferation was measured by MTT assay. The effect of varying doses of taurine, EGCG and genistein were compared to (I) untreated control cells; or cells treated with (II) 0.015 mg/mL taurine, 0.0175 mg/mL EGCG and 0.0035 mg/mL genistein; (III) 0.03 mg/mL taurine, 0.035 mg/mL EGCG and 0.007 mg/mL genistein; or (IV) 0.06 mg/mL taurine, 0.07 mg/mL EGCG and 0.014 mg/mL genistein. Data are presented as the mean ± S.E.; * p <0.05 vs. group I (untreated control).
Mentions: The anti-proliferative effect of combined taurine, EGCG and genistein treatment in HSC-T6 cells was determined by measuring cell viability using MTT assay. After treatment with different doses of the drug combination, the proliferation of all treated groups was significantly reduced compared to control HSCs. As shown in Figure 1, HSC-T6 cell proliferation was remarkably inhibited by the drug combinations.

Bottom Line: Combined drug treatment significantly inhibited cell proliferation and TGF-β1, Col-I, TIMP-1 and TIMP-2 mRNA expression in activated HSC-T6 cells, while the expression of MMP-2 mRNA increased.In conclusion, combined drug treatment with taurine, epigallocatechin gallate and genistein can inhibit HSC proliferation, and impact fibrosis-related gene and protein expression.The antifibrotic effects of this drug combination may be due to its effects on the expression of fibrogenic genes.

View Article: PubMed Central - PubMed

Affiliation: Guangxi University Library, Guangxi University, Nanning 530004, Guangxi, China. lminggx@163.com.

ABSTRACT
Hepatic fibrogenesis involves the activation of hepatic stellate cells (HSCs), which synthesize excess extracellular matrix and contribute to the development of liver fibrosis. In a prior study we tested the effect of combined treatment with taurine, epigallocatechin gallate and genistein on the development of alcohol-induced liver fibrosis in vitro. In this study, the biological activity of the combination of these molecules was assessed by measuring its effect on cell proliferation, fibrosis-related gene expression, and proteomic expression profiling in the activated HSC cell line, HSC-T6. HSC-T6 cells were incubated with different concentrations of the drug combination taurine, epigallocatechin gallate and genistein. Cell proliferation was evaluated by MTT assay. Transforming growth factor β1 (TGF-β1), collagen type I (Col-I), matrix metalloproteinase-2 (MMP-2), and tissue inhibitor of metalloproteinases 1 and 2 (TIMP-1 and TIMP-2) mRNA were analyzed by semi-quantitative reverse-transcription PCR. Proteomic profiling of HSC-T6 cells was also performed by SELDI-TOF-MS. Combined drug treatment significantly inhibited cell proliferation and TGF-β1, Col-I, TIMP-1 and TIMP-2 mRNA expression in activated HSC-T6 cells, while the expression of MMP-2 mRNA increased. A total of 176 protein m/z peaks were identified. The intensities of 10 protein peaks were downregulated and two protein peaks were upregulated in HSC-T6 cells after combined drug treatment. In conclusion, combined drug treatment with taurine, epigallocatechin gallate and genistein can inhibit HSC proliferation, and impact fibrosis-related gene and protein expression. The antifibrotic effects of this drug combination may be due to its effects on the expression of fibrogenic genes.

Show MeSH
Related in: MedlinePlus