Limits...
Significant overexpression of DVL1 in Taiwanese colorectal cancer patients with liver metastasis.

Huang MY, Yen LC, Liu HC, Liu PP, Chung FY, Wang TN, Wang JY, Lin SR - Int J Mol Sci (2013)

Bottom Line: Microarray and bioinformatics analysis of 10 CRC cancer tissue specimens compared with normal adjacent tissues revealed that 31 genes were up-regulated (gene expression ratio of cancer tissue to paired normal tissue > 2) in the cancer patients.Using univariate analysis and multivariate proportional hazards regression analysis, we found that DVL1 mRNA overexpression had a significant, independent predictive value for liver metastasis in CRC patients (OR: 5.764; 95% CI: 2.588-12.837; p < 0.0001 on univariate analysis; OR: 3.768; 95% CI: 1.469-9.665; p = 0.006 on multivariate analysis).Our experimental results demonstrated that DVL1 is significantly overexpressed in CRC patients with liver metastasis, leading us to conclude that DVL1 could be a potential prognostic and predictive marker for CRC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan. cy614112@ms14.hinet.net.

ABSTRACT
Undetected micrometastasis plays a key role in the metastasis of cancer in colorectal cancer (CRC) patients. The aim of this study is to identify a biomarker of CRC patients with liver metastasis through the detection of circulating tumor cells (CTCs). Microarray and bioinformatics analysis of 10 CRC cancer tissue specimens compared with normal adjacent tissues revealed that 31 genes were up-regulated (gene expression ratio of cancer tissue to paired normal tissue > 2) in the cancer patients. We used a weighted enzymatic chip array (WEnCA) including 31 prognosis-related genes to investigate CTCs in 214 postoperative stage I-III CRC patients and to analyze the correlation between gene expression and clinico-pathological parameters. We employed the immunohistochemistry (IHC) method with polyclonal mouse antibody against DVL1 to detect DVL1 expression in 60 CRC patients. CRC liver metastasis occurred in 19.16% (41/214) of the patients. Using univariate analysis and multivariate proportional hazards regression analysis, we found that DVL1 mRNA overexpression had a significant, independent predictive value for liver metastasis in CRC patients (OR: 5.764; 95% CI: 2.588-12.837; p < 0.0001 on univariate analysis; OR: 3.768; 95% CI: 1.469-9.665; p = 0.006 on multivariate analysis). IHC staining of the immunoreactivity of DVL1 showed that DVL1 was localized in the cytoplasm of CRC cells. High expression of DVL1 was observed in 55% (33/60) of CRC tumor specimens and was associated significantly with tumor depth, perineural invasion and liver metastasis status (all p < 0.05). Our experimental results demonstrated that DVL1 is significantly overexpressed in CRC patients with liver metastasis, leading us to conclude that DVL1 could be a potential prognostic and predictive marker for CRC patients.

Show MeSH

Related in: MedlinePlus

Immunohistochemistry (IHC) method with polyclonal mouse antibody against DVL1 was performed to detect DVL1 expression in 60 colorectal cancer (CRC) tumor specimens. (A) Blank; (B) Negative staining (score 0); (C) Weakly positive staining (score 2); (D) Weakly positive staining (score 6); (E) Strongly positive staining (score 8); (F) Strongly positive staining (score 12) in CRC tissues. Magnification, ×100.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3821627&req=5

f1-ijms-14-20492: Immunohistochemistry (IHC) method with polyclonal mouse antibody against DVL1 was performed to detect DVL1 expression in 60 colorectal cancer (CRC) tumor specimens. (A) Blank; (B) Negative staining (score 0); (C) Weakly positive staining (score 2); (D) Weakly positive staining (score 6); (E) Strongly positive staining (score 8); (F) Strongly positive staining (score 12) in CRC tissues. Magnification, ×100.

Mentions: We utilized DVL1 antibody to perform IHC staining in the collected tissue biopsies of 60 CRC patients, to localize and analyze DVL1 expression in CRC tissues. A positive yellowish-brown appearance after tissue dyeing indicated that DVL1 was overexpressed (Figure 1), and the immunoreactivity (yellowish-brown appearance) was localized in the cytoplasm of CRC tumor cells. From the correlation between DVL1 high expression and clinicopathologic features of 60 CRC patients using univariate analyses, we observed that a deeper tumor invasion (p = 0.036), the presence of perineural invasion (p = 0.028), and the presence of liver metastasis (p = 0.013) showed significant correlation (Table 5).


Significant overexpression of DVL1 in Taiwanese colorectal cancer patients with liver metastasis.

Huang MY, Yen LC, Liu HC, Liu PP, Chung FY, Wang TN, Wang JY, Lin SR - Int J Mol Sci (2013)

Immunohistochemistry (IHC) method with polyclonal mouse antibody against DVL1 was performed to detect DVL1 expression in 60 colorectal cancer (CRC) tumor specimens. (A) Blank; (B) Negative staining (score 0); (C) Weakly positive staining (score 2); (D) Weakly positive staining (score 6); (E) Strongly positive staining (score 8); (F) Strongly positive staining (score 12) in CRC tissues. Magnification, ×100.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3821627&req=5

f1-ijms-14-20492: Immunohistochemistry (IHC) method with polyclonal mouse antibody against DVL1 was performed to detect DVL1 expression in 60 colorectal cancer (CRC) tumor specimens. (A) Blank; (B) Negative staining (score 0); (C) Weakly positive staining (score 2); (D) Weakly positive staining (score 6); (E) Strongly positive staining (score 8); (F) Strongly positive staining (score 12) in CRC tissues. Magnification, ×100.
Mentions: We utilized DVL1 antibody to perform IHC staining in the collected tissue biopsies of 60 CRC patients, to localize and analyze DVL1 expression in CRC tissues. A positive yellowish-brown appearance after tissue dyeing indicated that DVL1 was overexpressed (Figure 1), and the immunoreactivity (yellowish-brown appearance) was localized in the cytoplasm of CRC tumor cells. From the correlation between DVL1 high expression and clinicopathologic features of 60 CRC patients using univariate analyses, we observed that a deeper tumor invasion (p = 0.036), the presence of perineural invasion (p = 0.028), and the presence of liver metastasis (p = 0.013) showed significant correlation (Table 5).

Bottom Line: Microarray and bioinformatics analysis of 10 CRC cancer tissue specimens compared with normal adjacent tissues revealed that 31 genes were up-regulated (gene expression ratio of cancer tissue to paired normal tissue > 2) in the cancer patients.Using univariate analysis and multivariate proportional hazards regression analysis, we found that DVL1 mRNA overexpression had a significant, independent predictive value for liver metastasis in CRC patients (OR: 5.764; 95% CI: 2.588-12.837; p < 0.0001 on univariate analysis; OR: 3.768; 95% CI: 1.469-9.665; p = 0.006 on multivariate analysis).Our experimental results demonstrated that DVL1 is significantly overexpressed in CRC patients with liver metastasis, leading us to conclude that DVL1 could be a potential prognostic and predictive marker for CRC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan. cy614112@ms14.hinet.net.

ABSTRACT
Undetected micrometastasis plays a key role in the metastasis of cancer in colorectal cancer (CRC) patients. The aim of this study is to identify a biomarker of CRC patients with liver metastasis through the detection of circulating tumor cells (CTCs). Microarray and bioinformatics analysis of 10 CRC cancer tissue specimens compared with normal adjacent tissues revealed that 31 genes were up-regulated (gene expression ratio of cancer tissue to paired normal tissue > 2) in the cancer patients. We used a weighted enzymatic chip array (WEnCA) including 31 prognosis-related genes to investigate CTCs in 214 postoperative stage I-III CRC patients and to analyze the correlation between gene expression and clinico-pathological parameters. We employed the immunohistochemistry (IHC) method with polyclonal mouse antibody against DVL1 to detect DVL1 expression in 60 CRC patients. CRC liver metastasis occurred in 19.16% (41/214) of the patients. Using univariate analysis and multivariate proportional hazards regression analysis, we found that DVL1 mRNA overexpression had a significant, independent predictive value for liver metastasis in CRC patients (OR: 5.764; 95% CI: 2.588-12.837; p < 0.0001 on univariate analysis; OR: 3.768; 95% CI: 1.469-9.665; p = 0.006 on multivariate analysis). IHC staining of the immunoreactivity of DVL1 showed that DVL1 was localized in the cytoplasm of CRC cells. High expression of DVL1 was observed in 55% (33/60) of CRC tumor specimens and was associated significantly with tumor depth, perineural invasion and liver metastasis status (all p < 0.05). Our experimental results demonstrated that DVL1 is significantly overexpressed in CRC patients with liver metastasis, leading us to conclude that DVL1 could be a potential prognostic and predictive marker for CRC patients.

Show MeSH
Related in: MedlinePlus