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Achyranthes bidentata polypeptides reduces oxidative stress and exerts protective effects against myocardial ischemic/reperfusion injury in rats.

Tie R, Ji L, Nan Y, Wang W, Liang X, Tian F, Xing W, Zhu M, Li R, Zhang H - Int J Mol Sci (2013)

Bottom Line: ABPP preconditioning for one week significantly improved cardiac function following MI/R.PI3K inhibitor wortmannin blocked Akt activation, and abolished ABPP-stimulated anti-oxidant effect and cardioprotection.Our study demonstrated for the first time that ABPP reduces oxidative stress and exerts cardioprotection against MI/R injury in rats.

View Article: PubMed Central - PubMed

Affiliation: Experiment Teaching Center, Fourth Military Medical University, Xi'an 710032, China. mz_zhu@fmmu.edu.cn.

ABSTRACT
Achyranthes bidentata, a Chinese medicinal herb, is reported to be neuroprotective. However, its role in cardioprotection remains largely unknown. Our present study aimed to investigate the effects of Achyranthes bidentata polypeptides (ABPP) preconditioning on myocardial ischemia/reperfusion (MI/R) injury and to test the possible mechanisms. Rats were treated with ABPP (10 mg/kg/d, i.p.) or saline once daily for one week. Afterward, all the animals were subjected to 30 min of myocardial ischemia followed by 4 h of reperfusion. ABPP preconditioning for one week significantly improved cardiac function following MI/R. Meanwhile, ABPP reduced infarct size, plasma creatine kinase (CK)/lactate dehydrogenase (LDH) activities and myocardial apoptosis at the end of reperfusion in rat hearts. Moreover, ABPP preconditioning significantly inhibited superoxide generation, gp91phox expression, malonaldialdehyde formation and enhanced superoxide dismutase activity in I/R hearts. Furthermore, ABPP treatment inhibited PTEN expression and increased Akt phosphorylation in I/R rat heart. PI3K inhibitor wortmannin blocked Akt activation, and abolished ABPP-stimulated anti-oxidant effect and cardioprotection. Our study demonstrated for the first time that ABPP reduces oxidative stress and exerts cardioprotection against MI/R injury in rats. Inhibition of PTEN and activation of Akt may contribute to the anti-oxidant capacity and cardioprotection of ABPP.

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Achyranthes bidentata polypeptides (ABPP) preconditioning reduced oxidative stress in rats subjected to myocardial ischemia/reperfusion. (A) Cardiac superoxide generation; (B) gp91phox expression. Top images: representative blots; (C) Myocardial malondialdehyde (MDA) contents; (D) Myocardial superoxide dismutase (SOD) activity. MI/R, myocardial ischemia/reperfusion (30 min/4 h); Sham, sham-operated; ABPP, Achyranthes bidentata polypeptides. Values presented are means ± SEM. n = 8/group. *p < 0.05, **p < 0.01 versus Sham, #p < 0.05versus MI/R.
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f4-ijms-14-19792: Achyranthes bidentata polypeptides (ABPP) preconditioning reduced oxidative stress in rats subjected to myocardial ischemia/reperfusion. (A) Cardiac superoxide generation; (B) gp91phox expression. Top images: representative blots; (C) Myocardial malondialdehyde (MDA) contents; (D) Myocardial superoxide dismutase (SOD) activity. MI/R, myocardial ischemia/reperfusion (30 min/4 h); Sham, sham-operated; ABPP, Achyranthes bidentata polypeptides. Values presented are means ± SEM. n = 8/group. *p < 0.05, **p < 0.01 versus Sham, #p < 0.05versus MI/R.

Mentions: As seen in Figure 4A, compared with sham-operated hearts, I/R myocardium showed a significant increase in superoxide content (p < 0.01, Figure 4B), and ABPP preconditioning decreased superoxide accumulation (p < 0.05 vs. MI/R). Afterwards, we determined gp91phox expression, a major component of NADPH oxidase that is the most important superoxide-producing enzyme in I/R heart. As expected, ABPP treatment markedly decreased MI/R-stimulated gp91phox expression (p < 0.05, Figure 4B). In addition, MDA was observed as a biomarker to measure the level of oxidative stress. There was a robust increase in MDA production in I/R hearts compared with the sham group, and treatment with ABPP reduced this harmful effect (Figure 4C). In contrast, antioxidant enzyme SOD activity in cardiac tissue was also increased in I/R rats with ABPP preconditioning (Figure 4D). These results demonstrated that ABPP reduced superoxide overproduction and decreased oxidative stress in I/R hearts.


Achyranthes bidentata polypeptides reduces oxidative stress and exerts protective effects against myocardial ischemic/reperfusion injury in rats.

Tie R, Ji L, Nan Y, Wang W, Liang X, Tian F, Xing W, Zhu M, Li R, Zhang H - Int J Mol Sci (2013)

Achyranthes bidentata polypeptides (ABPP) preconditioning reduced oxidative stress in rats subjected to myocardial ischemia/reperfusion. (A) Cardiac superoxide generation; (B) gp91phox expression. Top images: representative blots; (C) Myocardial malondialdehyde (MDA) contents; (D) Myocardial superoxide dismutase (SOD) activity. MI/R, myocardial ischemia/reperfusion (30 min/4 h); Sham, sham-operated; ABPP, Achyranthes bidentata polypeptides. Values presented are means ± SEM. n = 8/group. *p < 0.05, **p < 0.01 versus Sham, #p < 0.05versus MI/R.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3821587&req=5

f4-ijms-14-19792: Achyranthes bidentata polypeptides (ABPP) preconditioning reduced oxidative stress in rats subjected to myocardial ischemia/reperfusion. (A) Cardiac superoxide generation; (B) gp91phox expression. Top images: representative blots; (C) Myocardial malondialdehyde (MDA) contents; (D) Myocardial superoxide dismutase (SOD) activity. MI/R, myocardial ischemia/reperfusion (30 min/4 h); Sham, sham-operated; ABPP, Achyranthes bidentata polypeptides. Values presented are means ± SEM. n = 8/group. *p < 0.05, **p < 0.01 versus Sham, #p < 0.05versus MI/R.
Mentions: As seen in Figure 4A, compared with sham-operated hearts, I/R myocardium showed a significant increase in superoxide content (p < 0.01, Figure 4B), and ABPP preconditioning decreased superoxide accumulation (p < 0.05 vs. MI/R). Afterwards, we determined gp91phox expression, a major component of NADPH oxidase that is the most important superoxide-producing enzyme in I/R heart. As expected, ABPP treatment markedly decreased MI/R-stimulated gp91phox expression (p < 0.05, Figure 4B). In addition, MDA was observed as a biomarker to measure the level of oxidative stress. There was a robust increase in MDA production in I/R hearts compared with the sham group, and treatment with ABPP reduced this harmful effect (Figure 4C). In contrast, antioxidant enzyme SOD activity in cardiac tissue was also increased in I/R rats with ABPP preconditioning (Figure 4D). These results demonstrated that ABPP reduced superoxide overproduction and decreased oxidative stress in I/R hearts.

Bottom Line: ABPP preconditioning for one week significantly improved cardiac function following MI/R.PI3K inhibitor wortmannin blocked Akt activation, and abolished ABPP-stimulated anti-oxidant effect and cardioprotection.Our study demonstrated for the first time that ABPP reduces oxidative stress and exerts cardioprotection against MI/R injury in rats.

View Article: PubMed Central - PubMed

Affiliation: Experiment Teaching Center, Fourth Military Medical University, Xi'an 710032, China. mz_zhu@fmmu.edu.cn.

ABSTRACT
Achyranthes bidentata, a Chinese medicinal herb, is reported to be neuroprotective. However, its role in cardioprotection remains largely unknown. Our present study aimed to investigate the effects of Achyranthes bidentata polypeptides (ABPP) preconditioning on myocardial ischemia/reperfusion (MI/R) injury and to test the possible mechanisms. Rats were treated with ABPP (10 mg/kg/d, i.p.) or saline once daily for one week. Afterward, all the animals were subjected to 30 min of myocardial ischemia followed by 4 h of reperfusion. ABPP preconditioning for one week significantly improved cardiac function following MI/R. Meanwhile, ABPP reduced infarct size, plasma creatine kinase (CK)/lactate dehydrogenase (LDH) activities and myocardial apoptosis at the end of reperfusion in rat hearts. Moreover, ABPP preconditioning significantly inhibited superoxide generation, gp91phox expression, malonaldialdehyde formation and enhanced superoxide dismutase activity in I/R hearts. Furthermore, ABPP treatment inhibited PTEN expression and increased Akt phosphorylation in I/R rat heart. PI3K inhibitor wortmannin blocked Akt activation, and abolished ABPP-stimulated anti-oxidant effect and cardioprotection. Our study demonstrated for the first time that ABPP reduces oxidative stress and exerts cardioprotection against MI/R injury in rats. Inhibition of PTEN and activation of Akt may contribute to the anti-oxidant capacity and cardioprotection of ABPP.

Show MeSH
Related in: MedlinePlus