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Three-dimensional reconstruction of oral tongue squamous cell carcinoma at invasion front.

Kudo T, Shimazu Y, Yagishita H, Izumo T, Soeno Y, Sato K, Taya Y, Aoba T - Int J Dent (2013)

Bottom Line: Serial sections (4  μ m thick) were double immunostained with pan-cytokeratin and Ki67 antibodies and digitized images were acquired using virtual microscopy.Direct visualization and quantitative assessment of the parenchymal-stromal border provide a new dimension in our understanding of OTSCC architecture.These 3D morphometric analyses also ascertained that cell invasion (individually and collectively) occurs at the deep invasive front of the OTSCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan ; Department of Pathology, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan.

ABSTRACT
We conducted three-dimensional (3D) reconstruction of oral tongue squamous cell carcinoma (OTSCC) using serial histological sections to visualize the architecture of invasive tumors. Fourteen OTSCC cases were collected from archival paraffin-embedded specimens. Based on a pathodiagnostic survey of whole cancer lesions, a core tissue specimen (3 mm in diameter) was dissected out from the deep invasion front using a paraffin tissue microarray. Serial sections (4  μ m thick) were double immunostained with pan-cytokeratin and Ki67 antibodies and digitized images were acquired using virtual microscopy. For 3D reconstruction, image registration and RGB color segmentation were automated using ImageJ software to avoid operator-dependent subjective errors. Based on the 3D tumor architecture, we classified the mode of invasion into four types: pushing and bulky architecture; trabecular architecture; diffuse spreading; and special forms. Direct visualization and quantitative assessment of the parenchymal-stromal border provide a new dimension in our understanding of OTSCC architecture. These 3D morphometric analyses also ascertained that cell invasion (individually and collectively) occurs at the deep invasive front of the OTSCC. These results demonstrate the advantages of histology-based 3D reconstruction for evaluating tumor architecture and its potential for a wide range of applications.

No MeSH data available.


Related in: MedlinePlus

(a, b) 3D view of the segmented tumor parenchymal (purple) and stromal (yellow) regions, respectively. (c) Enlargement of the CK-positive tumor architecture in the rectangle shown in (a), showing in detail the interconnected tumor texture and channels or holes corresponding to penetrating stromal strands. (d) Labyrinthine architecture of the tumor parenchymal-stromal border, which was virtually displayed in a plane of one voxel in width. Note that this 3D image was constructed using 30 serial sections to improve the visibility of the tortuous architecture. Bar = 0.5 mm.
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fig3: (a, b) 3D view of the segmented tumor parenchymal (purple) and stromal (yellow) regions, respectively. (c) Enlargement of the CK-positive tumor architecture in the rectangle shown in (a), showing in detail the interconnected tumor texture and channels or holes corresponding to penetrating stromal strands. (d) Labyrinthine architecture of the tumor parenchymal-stromal border, which was virtually displayed in a plane of one voxel in width. Note that this 3D image was constructed using 30 serial sections to improve the visibility of the tortuous architecture. Bar = 0.5 mm.

Mentions: Figure 3 shows an example of the segregation of the CK-positive tumor parenchyma (Figure 3(a)) and the adjacent stromal space (Figure 3(b)). In this OTSCC case (case C in Table 2), the segmented tumor mass appears bulky at low magnification, but a magnified 3D visualization reveals that the parenchymal-stromal border has a rough surface texture where small tumor cords and strands are connected to each other with narrow stromal penetration (Figure 3(c)). When the segmented parenchymal-stromal border was delineated in a virtual space as a plane of one voxel in width, the labyrinthine structure extending into the massive intratumor space can be fully appreciated (Figure 3(d)). As shown in Table 2, based on 3D morphometry, this bulky tumor architecture and its intricate tumor-host border correspond numerically to a parenchymal volume of 2.95 mm3, a border area of 61.4 mm2, and a surface area per volume of 97.5 mm−1.


Three-dimensional reconstruction of oral tongue squamous cell carcinoma at invasion front.

Kudo T, Shimazu Y, Yagishita H, Izumo T, Soeno Y, Sato K, Taya Y, Aoba T - Int J Dent (2013)

(a, b) 3D view of the segmented tumor parenchymal (purple) and stromal (yellow) regions, respectively. (c) Enlargement of the CK-positive tumor architecture in the rectangle shown in (a), showing in detail the interconnected tumor texture and channels or holes corresponding to penetrating stromal strands. (d) Labyrinthine architecture of the tumor parenchymal-stromal border, which was virtually displayed in a plane of one voxel in width. Note that this 3D image was constructed using 30 serial sections to improve the visibility of the tortuous architecture. Bar = 0.5 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818895&req=5

fig3: (a, b) 3D view of the segmented tumor parenchymal (purple) and stromal (yellow) regions, respectively. (c) Enlargement of the CK-positive tumor architecture in the rectangle shown in (a), showing in detail the interconnected tumor texture and channels or holes corresponding to penetrating stromal strands. (d) Labyrinthine architecture of the tumor parenchymal-stromal border, which was virtually displayed in a plane of one voxel in width. Note that this 3D image was constructed using 30 serial sections to improve the visibility of the tortuous architecture. Bar = 0.5 mm.
Mentions: Figure 3 shows an example of the segregation of the CK-positive tumor parenchyma (Figure 3(a)) and the adjacent stromal space (Figure 3(b)). In this OTSCC case (case C in Table 2), the segmented tumor mass appears bulky at low magnification, but a magnified 3D visualization reveals that the parenchymal-stromal border has a rough surface texture where small tumor cords and strands are connected to each other with narrow stromal penetration (Figure 3(c)). When the segmented parenchymal-stromal border was delineated in a virtual space as a plane of one voxel in width, the labyrinthine structure extending into the massive intratumor space can be fully appreciated (Figure 3(d)). As shown in Table 2, based on 3D morphometry, this bulky tumor architecture and its intricate tumor-host border correspond numerically to a parenchymal volume of 2.95 mm3, a border area of 61.4 mm2, and a surface area per volume of 97.5 mm−1.

Bottom Line: Serial sections (4  μ m thick) were double immunostained with pan-cytokeratin and Ki67 antibodies and digitized images were acquired using virtual microscopy.Direct visualization and quantitative assessment of the parenchymal-stromal border provide a new dimension in our understanding of OTSCC architecture.These 3D morphometric analyses also ascertained that cell invasion (individually and collectively) occurs at the deep invasive front of the OTSCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan ; Department of Pathology, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan.

ABSTRACT
We conducted three-dimensional (3D) reconstruction of oral tongue squamous cell carcinoma (OTSCC) using serial histological sections to visualize the architecture of invasive tumors. Fourteen OTSCC cases were collected from archival paraffin-embedded specimens. Based on a pathodiagnostic survey of whole cancer lesions, a core tissue specimen (3 mm in diameter) was dissected out from the deep invasion front using a paraffin tissue microarray. Serial sections (4  μ m thick) were double immunostained with pan-cytokeratin and Ki67 antibodies and digitized images were acquired using virtual microscopy. For 3D reconstruction, image registration and RGB color segmentation were automated using ImageJ software to avoid operator-dependent subjective errors. Based on the 3D tumor architecture, we classified the mode of invasion into four types: pushing and bulky architecture; trabecular architecture; diffuse spreading; and special forms. Direct visualization and quantitative assessment of the parenchymal-stromal border provide a new dimension in our understanding of OTSCC architecture. These 3D morphometric analyses also ascertained that cell invasion (individually and collectively) occurs at the deep invasive front of the OTSCC. These results demonstrate the advantages of histology-based 3D reconstruction for evaluating tumor architecture and its potential for a wide range of applications.

No MeSH data available.


Related in: MedlinePlus