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Extracellular Streptomyces vesicles: amphorae for survival and defence.

Schrempf H, Koebsch I, Walter S, Engelhardt H, Meschke H - Microb Biotechnol (2011)

Bottom Line: Our LC/MS analyses of generated tryptic peptides led to the identification of distinct proteins that carry often a predicted N-terminal signal peptide with a twin-arginine motif or lack a canonical signal sequence.The proteins are required for a range of processes: the acquisition of inorganic as well as organic phosphate, iron ions, and of distinct carbon sources, energy metabolism and redox balance, defence against oxidants and tellurites, the tailoring of actinorhodin, folding and assembly of proteins, establishment of turgor, and different signalling cascades.Our novel findings have immense implications for understanding new avenues of environmental biology of streptomycetes and for biotechnological applications.

View Article: PubMed Central - PubMed

Affiliation: FB Biology/Chemistry, Applied Genetics of Microorganisms, University Osnabrück, Barbarastr. 13, D-49069 Osnabrück, Germany. schrempf@biologie.uni-osnabrueck.de

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Mentions: M110 – a derivative of S. coelicolor A3(2) lacking the linear plasmid SCP1 – was inoculated with spores on plates containing agar with complete medium (see Experimental procedures). Following germination, substrate and aerial hyphae developed. Seven to 10 days later, droplets arose on top of the sporulated areas. The volume of visible droplets usually varied between 5 and 50 µl; their colour ranged between bright and darker blue (Fig. 1A).


Extracellular Streptomyces vesicles: amphorae for survival and defence.

Schrempf H, Koebsch I, Walter S, Engelhardt H, Meschke H - Microb Biotechnol (2011)

© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818868&req=5

Mentions: M110 – a derivative of S. coelicolor A3(2) lacking the linear plasmid SCP1 – was inoculated with spores on plates containing agar with complete medium (see Experimental procedures). Following germination, substrate and aerial hyphae developed. Seven to 10 days later, droplets arose on top of the sporulated areas. The volume of visible droplets usually varied between 5 and 50 µl; their colour ranged between bright and darker blue (Fig. 1A).

Bottom Line: Our LC/MS analyses of generated tryptic peptides led to the identification of distinct proteins that carry often a predicted N-terminal signal peptide with a twin-arginine motif or lack a canonical signal sequence.The proteins are required for a range of processes: the acquisition of inorganic as well as organic phosphate, iron ions, and of distinct carbon sources, energy metabolism and redox balance, defence against oxidants and tellurites, the tailoring of actinorhodin, folding and assembly of proteins, establishment of turgor, and different signalling cascades.Our novel findings have immense implications for understanding new avenues of environmental biology of streptomycetes and for biotechnological applications.

View Article: PubMed Central - PubMed

Affiliation: FB Biology/Chemistry, Applied Genetics of Microorganisms, University Osnabrück, Barbarastr. 13, D-49069 Osnabrück, Germany. schrempf@biologie.uni-osnabrueck.de

Show MeSH
Related in: MedlinePlus