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Disruption of the siderophore-binding desE receptor gene in Streptomyces coelicolor A3(2) results in impaired growth in spite of multiple iron-siderophore transport systems.

Tierrafría VH, Ramos-Aboites HE, Gosset G, Barona-Gómez F - Microb Biotechnol (2011)

Bottom Line: Here, disruption of the desE gene in S. coelicolor, and subsequent phenotypic analysis, is used to demonstrate a link between iron metabolism and physiological and morphological development.Streptomyces coelicolor desE mutants, isolated in both wild-type (M145) and a coelichelin biosynthesis and transport minus background (mutant W3), a second hydroxamate siderophore system only found in S. coelicolor and related species, resulted in impaired growth and lack of sporulation.The biotechnological and ecological implications of these observations are discussed.

View Article: PubMed Central - PubMed

Affiliation: Evolution of Metabolic Diversity Laboratory, Laboratorio Nacional de Genómica para la Biodiversidad (Langebio), CINVESTAV-IPN, Km 9.6 Libramiento Norte, Carretera Irapuato-León, Irapuato, C.P. 36822, México.

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Phenotypic characterization of the desE mutant V3. A total of 106 spores of both the parental strain W3 and the desE mutant V3 were used to inoculate R2YE agar plates with varying concentrations of the iron‐chelator 2,2′‐dipyridyl (0, 2, 20 and 200 µM). The plates were incubated at 30°C and growth was recorded daily for 6 days (141 h).
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f3: Phenotypic characterization of the desE mutant V3. A total of 106 spores of both the parental strain W3 and the desE mutant V3 were used to inoculate R2YE agar plates with varying concentrations of the iron‐chelator 2,2′‐dipyridyl (0, 2, 20 and 200 µM). The plates were incubated at 30°C and growth was recorded daily for 6 days (141 h).

Mentions: Disruption of desE dramatically affects growth of V3 and development in R2YE medium supplemented with the iron‐chelator 2,2′‐dipyridyl (Fig. 3). We have previously used this compound to reproduce an iron‐limited condition, even in rich media, with the concomitant induction of dFOs synthesis (Barona‐Gómez et al., 2006). Interestingly, impaired growth of V3 was recorded even without addition of the iron‐chelator, although the phenotype became more apparent as the concentration of 2,2′‐dipyridyl increased. An excess concentration of 200 µM was used for subsequent characterization of the mutant strains, both in liquid and in solid media, since this concentration ensures a phenotypic response related to iron limitation, even in M145 and W3.


Disruption of the siderophore-binding desE receptor gene in Streptomyces coelicolor A3(2) results in impaired growth in spite of multiple iron-siderophore transport systems.

Tierrafría VH, Ramos-Aboites HE, Gosset G, Barona-Gómez F - Microb Biotechnol (2011)

Phenotypic characterization of the desE mutant V3. A total of 106 spores of both the parental strain W3 and the desE mutant V3 were used to inoculate R2YE agar plates with varying concentrations of the iron‐chelator 2,2′‐dipyridyl (0, 2, 20 and 200 µM). The plates were incubated at 30°C and growth was recorded daily for 6 days (141 h).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818867&req=5

f3: Phenotypic characterization of the desE mutant V3. A total of 106 spores of both the parental strain W3 and the desE mutant V3 were used to inoculate R2YE agar plates with varying concentrations of the iron‐chelator 2,2′‐dipyridyl (0, 2, 20 and 200 µM). The plates were incubated at 30°C and growth was recorded daily for 6 days (141 h).
Mentions: Disruption of desE dramatically affects growth of V3 and development in R2YE medium supplemented with the iron‐chelator 2,2′‐dipyridyl (Fig. 3). We have previously used this compound to reproduce an iron‐limited condition, even in rich media, with the concomitant induction of dFOs synthesis (Barona‐Gómez et al., 2006). Interestingly, impaired growth of V3 was recorded even without addition of the iron‐chelator, although the phenotype became more apparent as the concentration of 2,2′‐dipyridyl increased. An excess concentration of 200 µM was used for subsequent characterization of the mutant strains, both in liquid and in solid media, since this concentration ensures a phenotypic response related to iron limitation, even in M145 and W3.

Bottom Line: Here, disruption of the desE gene in S. coelicolor, and subsequent phenotypic analysis, is used to demonstrate a link between iron metabolism and physiological and morphological development.Streptomyces coelicolor desE mutants, isolated in both wild-type (M145) and a coelichelin biosynthesis and transport minus background (mutant W3), a second hydroxamate siderophore system only found in S. coelicolor and related species, resulted in impaired growth and lack of sporulation.The biotechnological and ecological implications of these observations are discussed.

View Article: PubMed Central - PubMed

Affiliation: Evolution of Metabolic Diversity Laboratory, Laboratorio Nacional de Genómica para la Biodiversidad (Langebio), CINVESTAV-IPN, Km 9.6 Libramiento Norte, Carretera Irapuato-León, Irapuato, C.P. 36822, México.

Show MeSH
Related in: MedlinePlus