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Disruption of the siderophore-binding desE receptor gene in Streptomyces coelicolor A3(2) results in impaired growth in spite of multiple iron-siderophore transport systems.

Tierrafría VH, Ramos-Aboites HE, Gosset G, Barona-Gómez F - Microb Biotechnol (2011)

Bottom Line: Here, disruption of the desE gene in S. coelicolor, and subsequent phenotypic analysis, is used to demonstrate a link between iron metabolism and physiological and morphological development.Streptomyces coelicolor desE mutants, isolated in both wild-type (M145) and a coelichelin biosynthesis and transport minus background (mutant W3), a second hydroxamate siderophore system only found in S. coelicolor and related species, resulted in impaired growth and lack of sporulation.The biotechnological and ecological implications of these observations are discussed.

View Article: PubMed Central - PubMed

Affiliation: Evolution of Metabolic Diversity Laboratory, Laboratorio Nacional de Genómica para la Biodiversidad (Langebio), CINVESTAV-IPN, Km 9.6 Libramiento Norte, Carretera Irapuato-León, Irapuato, C.P. 36822, México.

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Current model for siderophore‐mediated iron acquisition in S. coelicolor. The proposed model is based in our previous report (Barona‐Gómez et al., 2006). Of particular relevance for this study are the specificities of the siderophore‐binding proteins DesE and CdtB, as well as the yet‐to‐be‐identified ABC and permease genes, encoding for the FOs import and dFOs export systems (marked as empty boxes).
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f2: Current model for siderophore‐mediated iron acquisition in S. coelicolor. The proposed model is based in our previous report (Barona‐Gómez et al., 2006). Of particular relevance for this study are the specificities of the siderophore‐binding proteins DesE and CdtB, as well as the yet‐to‐be‐identified ABC and permease genes, encoding for the FOs import and dFOs export systems (marked as empty boxes).

Mentions: Two different loci involved in transport and biosynthesis of FOs and dFOs, respectively, have been previously identified in Streptomyces coelicolor A3(2) (Barona‐Gómez et al., 2006; Bunet et al., 2006). These studies showed that the desEFABCD locus (Sco2780 to Sco2785) directs the synthesis of dFOs (desABCD), plus FO recognition (desE) and assimilation (desF), with a particular specificity towards FO‐E, whereas the cdt locus (Sco7400 to Sco7398 or cdtABC) encodes for a complete iron–siderophore importer with an apparent specificity towards FO‐B and related linear hydroxamate (xeno)siderophores, but not coelichelin. However, many of the components needed for proper export and import of dFOs and FOs, respectively, remain to be identified. Of particular relevance are the genes encoding for the transporters that allow translocation of dFOs and FOs through the bacterial membrane, which, in the case of FOs import, must interact with the presumed siderophore‐binding DesE receptor (Fig. 2).


Disruption of the siderophore-binding desE receptor gene in Streptomyces coelicolor A3(2) results in impaired growth in spite of multiple iron-siderophore transport systems.

Tierrafría VH, Ramos-Aboites HE, Gosset G, Barona-Gómez F - Microb Biotechnol (2011)

Current model for siderophore‐mediated iron acquisition in S. coelicolor. The proposed model is based in our previous report (Barona‐Gómez et al., 2006). Of particular relevance for this study are the specificities of the siderophore‐binding proteins DesE and CdtB, as well as the yet‐to‐be‐identified ABC and permease genes, encoding for the FOs import and dFOs export systems (marked as empty boxes).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818867&req=5

f2: Current model for siderophore‐mediated iron acquisition in S. coelicolor. The proposed model is based in our previous report (Barona‐Gómez et al., 2006). Of particular relevance for this study are the specificities of the siderophore‐binding proteins DesE and CdtB, as well as the yet‐to‐be‐identified ABC and permease genes, encoding for the FOs import and dFOs export systems (marked as empty boxes).
Mentions: Two different loci involved in transport and biosynthesis of FOs and dFOs, respectively, have been previously identified in Streptomyces coelicolor A3(2) (Barona‐Gómez et al., 2006; Bunet et al., 2006). These studies showed that the desEFABCD locus (Sco2780 to Sco2785) directs the synthesis of dFOs (desABCD), plus FO recognition (desE) and assimilation (desF), with a particular specificity towards FO‐E, whereas the cdt locus (Sco7400 to Sco7398 or cdtABC) encodes for a complete iron–siderophore importer with an apparent specificity towards FO‐B and related linear hydroxamate (xeno)siderophores, but not coelichelin. However, many of the components needed for proper export and import of dFOs and FOs, respectively, remain to be identified. Of particular relevance are the genes encoding for the transporters that allow translocation of dFOs and FOs through the bacterial membrane, which, in the case of FOs import, must interact with the presumed siderophore‐binding DesE receptor (Fig. 2).

Bottom Line: Here, disruption of the desE gene in S. coelicolor, and subsequent phenotypic analysis, is used to demonstrate a link between iron metabolism and physiological and morphological development.Streptomyces coelicolor desE mutants, isolated in both wild-type (M145) and a coelichelin biosynthesis and transport minus background (mutant W3), a second hydroxamate siderophore system only found in S. coelicolor and related species, resulted in impaired growth and lack of sporulation.The biotechnological and ecological implications of these observations are discussed.

View Article: PubMed Central - PubMed

Affiliation: Evolution of Metabolic Diversity Laboratory, Laboratorio Nacional de Genómica para la Biodiversidad (Langebio), CINVESTAV-IPN, Km 9.6 Libramiento Norte, Carretera Irapuato-León, Irapuato, C.P. 36822, México.

Show MeSH
Related in: MedlinePlus