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Disruption of the siderophore-binding desE receptor gene in Streptomyces coelicolor A3(2) results in impaired growth in spite of multiple iron-siderophore transport systems.

Tierrafría VH, Ramos-Aboites HE, Gosset G, Barona-Gómez F - Microb Biotechnol (2011)

Bottom Line: Here, disruption of the desE gene in S. coelicolor, and subsequent phenotypic analysis, is used to demonstrate a link between iron metabolism and physiological and morphological development.Streptomyces coelicolor desE mutants, isolated in both wild-type (M145) and a coelichelin biosynthesis and transport minus background (mutant W3), a second hydroxamate siderophore system only found in S. coelicolor and related species, resulted in impaired growth and lack of sporulation.The biotechnological and ecological implications of these observations are discussed.

View Article: PubMed Central - PubMed

Affiliation: Evolution of Metabolic Diversity Laboratory, Laboratorio Nacional de Genómica para la Biodiversidad (Langebio), CINVESTAV-IPN, Km 9.6 Libramiento Norte, Carretera Irapuato-León, Irapuato, C.P. 36822, México.

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Molecular structures of the three hydroxamate‐type siderophores produced by S. coelicolor. Desferrioxamine B (dFO‐B), desferrioxamine E (dFO‐E) and coelichelin (Cch).
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f1: Molecular structures of the three hydroxamate‐type siderophores produced by S. coelicolor. Desferrioxamine B (dFO‐B), desferrioxamine E (dFO‐E) and coelichelin (Cch).

Mentions: Siderophore‐mediated iron acquisition by Streptomyces species, including siderophore biosynthesis and its regulation, and aspects of iron–siderophore uptake have recently received increased attention (Barona‐Gómez et al., 2004; Flores and Martín, 2004; Barona‐Gómez et al., 2006; Bunet et al., 2006; Kadi et al., 2007; Tunca et al., 2007; 2009). These studies have paved the way for developing the main siderophores produced by these organisms, i.e. the desferrioxamines (dFOs, Fig. 1), into many novel biotechnological processes, from agriculture and bioremediation (Dimkpa et al., 2008a,b; 2009; Fialho de Oliveira et al., 2010; Rajkumar et al., 2010) to medicine (Dayani et al., 2004; Warshawsky et al., 2005; Banin et al., 2008). Despite the isolation of several siderophores produced by members of this genus, including the novel hydroxamate siderophore coelichelin (Cch, Fig. 1; Lautru et al., 2005; Dimkpa et al., 2008b) and catechol siderophores previously isolated from other organisms (Fiedler et al., 2001; Patzer and Braun, 2009), only the dFOs have found biotechnological applications.


Disruption of the siderophore-binding desE receptor gene in Streptomyces coelicolor A3(2) results in impaired growth in spite of multiple iron-siderophore transport systems.

Tierrafría VH, Ramos-Aboites HE, Gosset G, Barona-Gómez F - Microb Biotechnol (2011)

Molecular structures of the three hydroxamate‐type siderophores produced by S. coelicolor. Desferrioxamine B (dFO‐B), desferrioxamine E (dFO‐E) and coelichelin (Cch).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818867&req=5

f1: Molecular structures of the three hydroxamate‐type siderophores produced by S. coelicolor. Desferrioxamine B (dFO‐B), desferrioxamine E (dFO‐E) and coelichelin (Cch).
Mentions: Siderophore‐mediated iron acquisition by Streptomyces species, including siderophore biosynthesis and its regulation, and aspects of iron–siderophore uptake have recently received increased attention (Barona‐Gómez et al., 2004; Flores and Martín, 2004; Barona‐Gómez et al., 2006; Bunet et al., 2006; Kadi et al., 2007; Tunca et al., 2007; 2009). These studies have paved the way for developing the main siderophores produced by these organisms, i.e. the desferrioxamines (dFOs, Fig. 1), into many novel biotechnological processes, from agriculture and bioremediation (Dimkpa et al., 2008a,b; 2009; Fialho de Oliveira et al., 2010; Rajkumar et al., 2010) to medicine (Dayani et al., 2004; Warshawsky et al., 2005; Banin et al., 2008). Despite the isolation of several siderophores produced by members of this genus, including the novel hydroxamate siderophore coelichelin (Cch, Fig. 1; Lautru et al., 2005; Dimkpa et al., 2008b) and catechol siderophores previously isolated from other organisms (Fiedler et al., 2001; Patzer and Braun, 2009), only the dFOs have found biotechnological applications.

Bottom Line: Here, disruption of the desE gene in S. coelicolor, and subsequent phenotypic analysis, is used to demonstrate a link between iron metabolism and physiological and morphological development.Streptomyces coelicolor desE mutants, isolated in both wild-type (M145) and a coelichelin biosynthesis and transport minus background (mutant W3), a second hydroxamate siderophore system only found in S. coelicolor and related species, resulted in impaired growth and lack of sporulation.The biotechnological and ecological implications of these observations are discussed.

View Article: PubMed Central - PubMed

Affiliation: Evolution of Metabolic Diversity Laboratory, Laboratorio Nacional de Genómica para la Biodiversidad (Langebio), CINVESTAV-IPN, Km 9.6 Libramiento Norte, Carretera Irapuato-León, Irapuato, C.P. 36822, México.

Show MeSH
Related in: MedlinePlus