Deletion of the signalling molecule synthase ScbA has pleiotropic effects on secondary metabolite biosynthesis, morphological differentiation and primary metabolism in Streptomyces coelicolor A3(2).
Bottom Line: Our study also revealed, for the first time, that the secondary metabolite cluster responsible for synthesis of the siderophore desferrioxamine is under the control of SCB signalling.Moreover, expression of the genes encoding enzymes for primary metabolism pathways, which supply antibiotic precursors and genes for morphological differentiation, was found shifted earlier in time in the mutant.In conclusion, our time series analysis demonstrates new details of the regulatory effects of the γ-butyrolactone system in Streptomyces.
Affiliation: Department of Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, the Netherlands.Show MeSH
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Mentions: Our group has recently predicted the presence of small non‐coding RNAs (ncRNAs) in inter‐ and intragenic loci in the S. coelicolor genome and experimentally confirmed the presence of 13 antisense ncRNAs (D'Alia et al., 2009). Because of technical reasons (the microarray in this study is PCR‐based) it was not possible to separately analyse the messenger and antisense transcripts. Nevertheless, 11 short coding sequences (maximum length 300 bp), which we predicted as ncRNA and which are annotated as encoding unknown or hypothetical proteins, were strongly upregulated in the γ‐butyrolactone‐impaired mutant at TP4 (Fig. 7).
Affiliation: Department of Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, the Netherlands.