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Deletion of the signalling molecule synthase ScbA has pleiotropic effects on secondary metabolite biosynthesis, morphological differentiation and primary metabolism in Streptomyces coelicolor A3(2).

D'Alia D, Eggle D, Nieselt K, Hu WS, Breitling R, Takano E - Microb Biotechnol (2010)

Bottom Line: Our study also revealed, for the first time, that the secondary metabolite cluster responsible for synthesis of the siderophore desferrioxamine is under the control of SCB signalling.Moreover, expression of the genes encoding enzymes for primary metabolism pathways, which supply antibiotic precursors and genes for morphological differentiation, was found shifted earlier in time in the mutant.In conclusion, our time series analysis demonstrates new details of the regulatory effects of the γ-butyrolactone system in Streptomyces.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, the Netherlands.

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Expression profiles of morphological differentiation genes and conservon 9. The average expression profiles of a set of morphological differentiation genes and the conservon 9 [cvn9 (rar)] are plotted for M145 (dark line) and the ΔscbA mutant (light line). A list of the genes and their expression data are included in Table S3.
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f6: Expression profiles of morphological differentiation genes and conservon 9. The average expression profiles of a set of morphological differentiation genes and the conservon 9 [cvn9 (rar)] are plotted for M145 (dark line) and the ΔscbA mutant (light line). A list of the genes and their expression data are included in Table S3.

Mentions: Our microarray analysis showed that the expression profile of ace (for conversion of pyruvate to acetyl‐CoA), the ACCase complex (from acetyl‐CoA to malonyl‐CoA) and the PPP (pentose phosphate pathway) genes is shifted one time point earlier in M751 compared with the wild type (Fig. 5). This pattern is weak for individual genes, but highly consistent across the set of genes involved. Similarly, although to a somewhat less extent, the gene expression of the glycolysis pathway was also shifted (Fig. 5). Streptomyces coelicolor has been shown to differentiate in liquid media (Manteca et al., 2008). A set of genes involved in morphological differentiation were found expressed and appeared to be shifted to one time point earlier in M751 (Fig. 6). These genes are ramS, encoding for SapB (Kodani et al., 2004), nepA (de Jong et al., 2009), chpH (Claessen et al., 2003), bldG and sigK (Mao et al., 2009a; Parashar et al., 2009). Another cluster showing a shifted expression profile to one time point earlier in the mutant compared with the wild type was the conservon 9 (Fig. 6). Conservons (cvns) are operons identified in S. coelicolor (Bentley et al., 2002) and conserved specifically in species belonging to Actinomycetales (Komatsu et al., 2006).


Deletion of the signalling molecule synthase ScbA has pleiotropic effects on secondary metabolite biosynthesis, morphological differentiation and primary metabolism in Streptomyces coelicolor A3(2).

D'Alia D, Eggle D, Nieselt K, Hu WS, Breitling R, Takano E - Microb Biotechnol (2010)

Expression profiles of morphological differentiation genes and conservon 9. The average expression profiles of a set of morphological differentiation genes and the conservon 9 [cvn9 (rar)] are plotted for M145 (dark line) and the ΔscbA mutant (light line). A list of the genes and their expression data are included in Table S3.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818864&req=5

f6: Expression profiles of morphological differentiation genes and conservon 9. The average expression profiles of a set of morphological differentiation genes and the conservon 9 [cvn9 (rar)] are plotted for M145 (dark line) and the ΔscbA mutant (light line). A list of the genes and their expression data are included in Table S3.
Mentions: Our microarray analysis showed that the expression profile of ace (for conversion of pyruvate to acetyl‐CoA), the ACCase complex (from acetyl‐CoA to malonyl‐CoA) and the PPP (pentose phosphate pathway) genes is shifted one time point earlier in M751 compared with the wild type (Fig. 5). This pattern is weak for individual genes, but highly consistent across the set of genes involved. Similarly, although to a somewhat less extent, the gene expression of the glycolysis pathway was also shifted (Fig. 5). Streptomyces coelicolor has been shown to differentiate in liquid media (Manteca et al., 2008). A set of genes involved in morphological differentiation were found expressed and appeared to be shifted to one time point earlier in M751 (Fig. 6). These genes are ramS, encoding for SapB (Kodani et al., 2004), nepA (de Jong et al., 2009), chpH (Claessen et al., 2003), bldG and sigK (Mao et al., 2009a; Parashar et al., 2009). Another cluster showing a shifted expression profile to one time point earlier in the mutant compared with the wild type was the conservon 9 (Fig. 6). Conservons (cvns) are operons identified in S. coelicolor (Bentley et al., 2002) and conserved specifically in species belonging to Actinomycetales (Komatsu et al., 2006).

Bottom Line: Our study also revealed, for the first time, that the secondary metabolite cluster responsible for synthesis of the siderophore desferrioxamine is under the control of SCB signalling.Moreover, expression of the genes encoding enzymes for primary metabolism pathways, which supply antibiotic precursors and genes for morphological differentiation, was found shifted earlier in time in the mutant.In conclusion, our time series analysis demonstrates new details of the regulatory effects of the γ-butyrolactone system in Streptomyces.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, the Netherlands.

Show MeSH