Limits...
Deletion of the signalling molecule synthase ScbA has pleiotropic effects on secondary metabolite biosynthesis, morphological differentiation and primary metabolism in Streptomyces coelicolor A3(2).

D'Alia D, Eggle D, Nieselt K, Hu WS, Breitling R, Takano E - Microb Biotechnol (2010)

Bottom Line: Our study also revealed, for the first time, that the secondary metabolite cluster responsible for synthesis of the siderophore desferrioxamine is under the control of SCB signalling.Moreover, expression of the genes encoding enzymes for primary metabolism pathways, which supply antibiotic precursors and genes for morphological differentiation, was found shifted earlier in time in the mutant.In conclusion, our time series analysis demonstrates new details of the regulatory effects of the γ-butyrolactone system in Streptomyces.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, the Netherlands.

Show MeSH
Quantitative RT‐PCR for selected genes. Expression profiles of genes of the red cluster [redD (SCO5877) and redQ (SCO5887)] (A), the act cluster [actII‐orf4 (SCO5085) and actIII (SCO5086)] (B), the cpk cluster [cpkE (SCO6277), cpkO (SCO6280) and scbR2 (SCO6286)] (C) are plotted for the wild type (dark line) and the ΔscbA mutant (light line). The expression for each gene is compared with the value in M145 at TP1, which is set as 1. Standard deviation at each time point is denoted in a thin light line.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3818864&req=5

f4: Quantitative RT‐PCR for selected genes. Expression profiles of genes of the red cluster [redD (SCO5877) and redQ (SCO5887)] (A), the act cluster [actII‐orf4 (SCO5085) and actIII (SCO5086)] (B), the cpk cluster [cpkE (SCO6277), cpkO (SCO6280) and scbR2 (SCO6286)] (C) are plotted for the wild type (dark line) and the ΔscbA mutant (light line). The expression for each gene is compared with the value in M145 at TP1, which is set as 1. Standard deviation at each time point is denoted in a thin light line.

Mentions: This group mainly consists of secondary metabolism gene clusters. Consistent with the observed earlier and more intense production of undecylprodigiosin, the microarray analysis revealed that the average of genes of the red biosynthetic cluster (SCO5877–5898) were expressed at higher values all along the growth curve and decreased in expression only very late at TP8 in the mutant, while in the wild‐type transcription peaked at TP4 and TP5 (transition phase) and decreased immediately afterwards (Fig. 3). In addition to undecylprodigiosin, scbA mutant M751 overproduced the blue‐pigmented actinorhodin, also consistent with an earlier report (Takano et al., 2001). The expression profile of its biosynthetic cluster act (SCO5071–5091) peaked at TP5 and TP6 (transition and early stationary phase) in the wild type, but was already almost twofold higher at TP4 in the mutant (Fig. 3). Earlier expression of redD and redQ (the main activator and the acyl carrier protein in the red cluster respectively), actII‐orf4 and actIII (the main activator and the ketoacyl reductase in the act cluster respectively) was confirmed by qRT‐PCR (Fig. 4A and B).


Deletion of the signalling molecule synthase ScbA has pleiotropic effects on secondary metabolite biosynthesis, morphological differentiation and primary metabolism in Streptomyces coelicolor A3(2).

D'Alia D, Eggle D, Nieselt K, Hu WS, Breitling R, Takano E - Microb Biotechnol (2010)

Quantitative RT‐PCR for selected genes. Expression profiles of genes of the red cluster [redD (SCO5877) and redQ (SCO5887)] (A), the act cluster [actII‐orf4 (SCO5085) and actIII (SCO5086)] (B), the cpk cluster [cpkE (SCO6277), cpkO (SCO6280) and scbR2 (SCO6286)] (C) are plotted for the wild type (dark line) and the ΔscbA mutant (light line). The expression for each gene is compared with the value in M145 at TP1, which is set as 1. Standard deviation at each time point is denoted in a thin light line.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818864&req=5

f4: Quantitative RT‐PCR for selected genes. Expression profiles of genes of the red cluster [redD (SCO5877) and redQ (SCO5887)] (A), the act cluster [actII‐orf4 (SCO5085) and actIII (SCO5086)] (B), the cpk cluster [cpkE (SCO6277), cpkO (SCO6280) and scbR2 (SCO6286)] (C) are plotted for the wild type (dark line) and the ΔscbA mutant (light line). The expression for each gene is compared with the value in M145 at TP1, which is set as 1. Standard deviation at each time point is denoted in a thin light line.
Mentions: This group mainly consists of secondary metabolism gene clusters. Consistent with the observed earlier and more intense production of undecylprodigiosin, the microarray analysis revealed that the average of genes of the red biosynthetic cluster (SCO5877–5898) were expressed at higher values all along the growth curve and decreased in expression only very late at TP8 in the mutant, while in the wild‐type transcription peaked at TP4 and TP5 (transition phase) and decreased immediately afterwards (Fig. 3). In addition to undecylprodigiosin, scbA mutant M751 overproduced the blue‐pigmented actinorhodin, also consistent with an earlier report (Takano et al., 2001). The expression profile of its biosynthetic cluster act (SCO5071–5091) peaked at TP5 and TP6 (transition and early stationary phase) in the wild type, but was already almost twofold higher at TP4 in the mutant (Fig. 3). Earlier expression of redD and redQ (the main activator and the acyl carrier protein in the red cluster respectively), actII‐orf4 and actIII (the main activator and the ketoacyl reductase in the act cluster respectively) was confirmed by qRT‐PCR (Fig. 4A and B).

Bottom Line: Our study also revealed, for the first time, that the secondary metabolite cluster responsible for synthesis of the siderophore desferrioxamine is under the control of SCB signalling.Moreover, expression of the genes encoding enzymes for primary metabolism pathways, which supply antibiotic precursors and genes for morphological differentiation, was found shifted earlier in time in the mutant.In conclusion, our time series analysis demonstrates new details of the regulatory effects of the γ-butyrolactone system in Streptomyces.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, the Netherlands.

Show MeSH