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Characterization of the 'pristinamycin supercluster' of Streptomyces pristinaespiralis.

Mast Y, Weber T, Gölz M, Ort-Winklbauer R, Gondran A, Wohlleben W, Schinko E - Microb Biotechnol (2010)

Bottom Line: Furthermore, the pristinamycin gene cluster was found to be interspersed by a cryptic secondary metabolite cluster, which probably codes for a glycosylated aromatic polyketide.Gene inactivation experiments revealed that this cluster has no influence on pristinamycin production.Overall, this work provides new insights into pristinamycin biosynthesis and the unique genetic organization of the pristinamycin gene region, which is the largest antibiotic 'supercluster' known so far.

View Article: PubMed Central - PubMed

Affiliation: Mikrobiologie/Biotechnologie, Interfakultäres Institut für Mikrobiologie und Infektionsmedizin, Fakultät für Biologie, Eberhard Karls Universität Tübingen, Auf der Morgenstelle 28, D-72076 Tübingen, Germany. yvonne.mast@biotech.uni-tuebingen.de

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Production profiles of S. pristinaespiralis Pr11 wild‐type (left) and mutant Mcpp8 (right) respectively. Pristinamycin PIA (Rt = 7.5 min), PIIA (Rt = 8.3 min) and PIIB (Rt = 7.7 min) were detected by UV‐Vis spectrometry (data not shown). Corresponding UV‐VIS spectra of retention region 7.5–7.7 are listed below. Multiple wavelength monitoring was performed at 210 (black), 230 (red), 260 (blue), 280 (green), 310 (light blue), 360 (magenta) and 435 (yellow) nm.
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f6: Production profiles of S. pristinaespiralis Pr11 wild‐type (left) and mutant Mcpp8 (right) respectively. Pristinamycin PIA (Rt = 7.5 min), PIIA (Rt = 8.3 min) and PIIB (Rt = 7.7 min) were detected by UV‐Vis spectrometry (data not shown). Corresponding UV‐VIS spectra of retention region 7.5–7.7 are listed below. Multiple wavelength monitoring was performed at 210 (black), 230 (red), 260 (blue), 280 (green), 310 (light blue), 360 (magenta) and 435 (yellow) nm.

Mentions: Taking the initial S. pristinaespiralis genome size of 7500 kb as predicted by Bamas‐Jacques and colleagues (1999), the cluster corresponds to 2.8% of the whole linear chromosome. Forty‐five genes covering a region of ∼ 120 kb were assigned as pristinamycin‐specific genes involved in the biosynthesis, regulation and resistance of pristinamycin, and 32 genes covering a region of ∼ 40 kb presumably code for the biosynthesis of a type II aromatic polyketide. The function of the remaining ∼ 50 kb, mainly located within the ∼ 90 kb interjacent sequence region, is unknown (see below; Figs 2 and 6, Tables 1 and 2). Altogether, this makes the pristinamycin biosynthetic gene region the largest antibiotic ‘supercluster’ that has been described so far.


Characterization of the 'pristinamycin supercluster' of Streptomyces pristinaespiralis.

Mast Y, Weber T, Gölz M, Ort-Winklbauer R, Gondran A, Wohlleben W, Schinko E - Microb Biotechnol (2010)

Production profiles of S. pristinaespiralis Pr11 wild‐type (left) and mutant Mcpp8 (right) respectively. Pristinamycin PIA (Rt = 7.5 min), PIIA (Rt = 8.3 min) and PIIB (Rt = 7.7 min) were detected by UV‐Vis spectrometry (data not shown). Corresponding UV‐VIS spectra of retention region 7.5–7.7 are listed below. Multiple wavelength monitoring was performed at 210 (black), 230 (red), 260 (blue), 280 (green), 310 (light blue), 360 (magenta) and 435 (yellow) nm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818860&req=5

f6: Production profiles of S. pristinaespiralis Pr11 wild‐type (left) and mutant Mcpp8 (right) respectively. Pristinamycin PIA (Rt = 7.5 min), PIIA (Rt = 8.3 min) and PIIB (Rt = 7.7 min) were detected by UV‐Vis spectrometry (data not shown). Corresponding UV‐VIS spectra of retention region 7.5–7.7 are listed below. Multiple wavelength monitoring was performed at 210 (black), 230 (red), 260 (blue), 280 (green), 310 (light blue), 360 (magenta) and 435 (yellow) nm.
Mentions: Taking the initial S. pristinaespiralis genome size of 7500 kb as predicted by Bamas‐Jacques and colleagues (1999), the cluster corresponds to 2.8% of the whole linear chromosome. Forty‐five genes covering a region of ∼ 120 kb were assigned as pristinamycin‐specific genes involved in the biosynthesis, regulation and resistance of pristinamycin, and 32 genes covering a region of ∼ 40 kb presumably code for the biosynthesis of a type II aromatic polyketide. The function of the remaining ∼ 50 kb, mainly located within the ∼ 90 kb interjacent sequence region, is unknown (see below; Figs 2 and 6, Tables 1 and 2). Altogether, this makes the pristinamycin biosynthetic gene region the largest antibiotic ‘supercluster’ that has been described so far.

Bottom Line: Furthermore, the pristinamycin gene cluster was found to be interspersed by a cryptic secondary metabolite cluster, which probably codes for a glycosylated aromatic polyketide.Gene inactivation experiments revealed that this cluster has no influence on pristinamycin production.Overall, this work provides new insights into pristinamycin biosynthesis and the unique genetic organization of the pristinamycin gene region, which is the largest antibiotic 'supercluster' known so far.

View Article: PubMed Central - PubMed

Affiliation: Mikrobiologie/Biotechnologie, Interfakultäres Institut für Mikrobiologie und Infektionsmedizin, Fakultät für Biologie, Eberhard Karls Universität Tübingen, Auf der Morgenstelle 28, D-72076 Tübingen, Germany. yvonne.mast@biotech.uni-tuebingen.de

Show MeSH
Related in: MedlinePlus