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Characterization of the 'pristinamycin supercluster' of Streptomyces pristinaespiralis.

Mast Y, Weber T, Gölz M, Ort-Winklbauer R, Gondran A, Wohlleben W, Schinko E - Microb Biotechnol (2010)

Bottom Line: Furthermore, the pristinamycin gene cluster was found to be interspersed by a cryptic secondary metabolite cluster, which probably codes for a glycosylated aromatic polyketide.Gene inactivation experiments revealed that this cluster has no influence on pristinamycin production.Overall, this work provides new insights into pristinamycin biosynthesis and the unique genetic organization of the pristinamycin gene region, which is the largest antibiotic 'supercluster' known so far.

View Article: PubMed Central - PubMed

Affiliation: Mikrobiologie/Biotechnologie, Interfakultäres Institut für Mikrobiologie und Infektionsmedizin, Fakultät für Biologie, Eberhard Karls Universität Tübingen, Auf der Morgenstelle 28, D-72076 Tübingen, Germany. yvonne.mast@biotech.uni-tuebingen.de

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Consensus tree of alignment of acyltransferase core domains of cis‐ and trans‐AT‐type polyketide synthases. Outgroup is FabD from E. coli.
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f4: Consensus tree of alignment of acyltransferase core domains of cis‐ and trans‐AT‐type polyketide synthases. Outgroup is FabD from E. coli.

Mentions: Phylogenetic analyses of acyltransferase domains of cis and trans‐AT PKS gene clusters demonstrate that SnaM is a member of a clade comprised exclusively of discrete ATs of trans‐AT‐PKS biosynthetic gene clusters such as LnmG from leinamycin biosynthesis (Cheng et al., 2003) or KirCI from kirromycin biosynthesis (Weber et al., 2008) (Fig. 4). It is noteworthy that these enzymes are phylogenetically more closely related to ATs from fatty acid synthases than to the internal AT domains of cis‐AT PKS.


Characterization of the 'pristinamycin supercluster' of Streptomyces pristinaespiralis.

Mast Y, Weber T, Gölz M, Ort-Winklbauer R, Gondran A, Wohlleben W, Schinko E - Microb Biotechnol (2010)

Consensus tree of alignment of acyltransferase core domains of cis‐ and trans‐AT‐type polyketide synthases. Outgroup is FabD from E. coli.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818860&req=5

f4: Consensus tree of alignment of acyltransferase core domains of cis‐ and trans‐AT‐type polyketide synthases. Outgroup is FabD from E. coli.
Mentions: Phylogenetic analyses of acyltransferase domains of cis and trans‐AT PKS gene clusters demonstrate that SnaM is a member of a clade comprised exclusively of discrete ATs of trans‐AT‐PKS biosynthetic gene clusters such as LnmG from leinamycin biosynthesis (Cheng et al., 2003) or KirCI from kirromycin biosynthesis (Weber et al., 2008) (Fig. 4). It is noteworthy that these enzymes are phylogenetically more closely related to ATs from fatty acid synthases than to the internal AT domains of cis‐AT PKS.

Bottom Line: Furthermore, the pristinamycin gene cluster was found to be interspersed by a cryptic secondary metabolite cluster, which probably codes for a glycosylated aromatic polyketide.Gene inactivation experiments revealed that this cluster has no influence on pristinamycin production.Overall, this work provides new insights into pristinamycin biosynthesis and the unique genetic organization of the pristinamycin gene region, which is the largest antibiotic 'supercluster' known so far.

View Article: PubMed Central - PubMed

Affiliation: Mikrobiologie/Biotechnologie, Interfakultäres Institut für Mikrobiologie und Infektionsmedizin, Fakultät für Biologie, Eberhard Karls Universität Tübingen, Auf der Morgenstelle 28, D-72076 Tübingen, Germany. yvonne.mast@biotech.uni-tuebingen.de

Show MeSH
Related in: MedlinePlus