Molecular insights on the biosynthesis of antitumour compounds by actinomycetes.
Bottom Line: The characterization of these clusters has represented, during the last 25 years, a great source of genes for the generation of novel derivatives by using combinatorial biosynthesis approaches: gene inactivation, gene expression, heterologous expression of the clusters or mutasynthesis.In addition, these techniques have been also applied to improve the production yields of natural and novel antitumour compounds.In this review we focus on some representative antitumour compounds produced by actinomycetes covering the genetic approaches used to isolate and validate their biosynthesis gene clusters, which finally led to generating novel derivatives and to improving the production yields.
Affiliation: Departamento de Biología Funcional and Instituto Universitario de Oncología del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, 33006 Oviedo, Spain.Show MeSH
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Mentions: Most of the genes in a cluster code for enzymes involved in the biosynthetic process, including the biosynthesis of precursor units, assembling of precursors, generation of other structural elements and tailoring modifications by attachment of the different structural elements or introduction of chemical functionalities (Fig. 4).
Affiliation: Departamento de Biología Funcional and Instituto Universitario de Oncología del Principado de Asturias (I.U.O.P.A), Universidad de Oviedo, 33006 Oviedo, Spain.