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Significance of glutathione peroxidase 1 and caudal-related homeodomain transcription factor in human gastric adenocarcinoma.

Han JJ, Xie de R, Wang LL, Liu YQ, Wu GF, Sun Q, Chen YX, Wei Y, Huang ZQ, Li HG - Gastroenterol Res Pract (2013)

Bottom Line: High expression of CDX2 was associated with differentiation, Lauren type, WHO type, extensive lymph node metastasis, and TNM of GAC.According to multivariate analysis, only lymph node metastasis, TNM stage, and CDX2 expression were independently associated with survival.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Zengcheng People's Hospital, Zengcheng City 511300, China ; Department of Pathology, Sun Yat-Sen Memorial Hospital, Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangdong Higher Education Institutes, Sun Yat-Sen University, Guangzhou 510120, China.

ABSTRACT
Aim. To investigate the expressions of glutathione peroxidase 1 (GPX1) and caudal-related homeodomain transcription factor (CDX2) in GAC and their correlation with clinicopathological features and tumor cell proliferation. Methods. The expressions of GPX1, CDX2, and Ki67 were immunohistochemically evaluated in 172 GAC specimens. The association of GPX1 and CDX2 with patient's clinicopathological features and Ki67 positive rate was analyzed statistically. Results. In 172 cases of GAC, the expression of GPX1 was weaker than that in adjacent normal mucosa, and the expression of CDX2 was higher than that in adjacent normal mucosa. High expression GPX1 strong-expression was associated with differentiation, Lauren type, WHO type and extensive lymph node metastasis of GAC. High expression of CDX2 was associated with differentiation, Lauren type, WHO type, extensive lymph node metastasis, and TNM of GAC. Survival curves showed that expressions of GPX1 and CDX2 were factors of good outcome (P = .03 and .02, resp.). According to multivariate analysis, only lymph node metastasis, TNM stage, and CDX2 expression were independently associated with survival. In addition, a strong association of GPX1 expression was noted with Ki67 and CDX2. Conclusions. The expression of GPX1 and CDX2 may play a role in the carcinogenesis, differentiation, and progression of GAC, and CDX2 may be an independent prognostic factor.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical staining in GAC and ANM tissue. (a) GPX1 expression in ANM tissue (score 3). (b) GPX1 expression in well-differentiated GAC (score 3). (c) GPX1 expression in well-differentiated GAC (score 2). (d) GPX1 expression in signet-ring cell GAC (score 0). (e) CDX2 expression in ANM tissue (negative). ((f) and (g)) CDX2 expression in well-differentiated GAC (positive). (h) CDX2 expression in signet-ring cell GAC (negative). (i) CDX2 expression in normal colon mucosa (positive). ((j) and (k)) Ki67 expression in well-differentiated GAC (negative). (l) Ki67 expression in signet-ring cell GAC (negative). Original magnification: ×200.
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fig1: Immunohistochemical staining in GAC and ANM tissue. (a) GPX1 expression in ANM tissue (score 3). (b) GPX1 expression in well-differentiated GAC (score 3). (c) GPX1 expression in well-differentiated GAC (score 2). (d) GPX1 expression in signet-ring cell GAC (score 0). (e) CDX2 expression in ANM tissue (negative). ((f) and (g)) CDX2 expression in well-differentiated GAC (positive). (h) CDX2 expression in signet-ring cell GAC (negative). (i) CDX2 expression in normal colon mucosa (positive). ((j) and (k)) Ki67 expression in well-differentiated GAC (negative). (l) Ki67 expression in signet-ring cell GAC (negative). Original magnification: ×200.

Mentions: Out of 172 gastric adenocarcinomas, 1 case (0.6%) had an index score of 3 (the case scored as a 3 was merged with those cases scored as a 2, statistically), 35 cases (20.3%) had index scores of 2, 96 cases (55.8%) had index scores of 1, and 40 cases (23.3%) had index scores of 0 (Figures 1(a)–1(d)). The total positive rate of GPX1 in all 172 cases of GAC was 76.7% (132/172). All 16 of the ANM tissues samples were scored as a 3. The expression of GPX1 in ANM tissues was significantly stronger than that in GAC (Z = −7.170, P = .000, r = .664). The higher positive rate of GPX1 was associated with better differentiation (χ2 = 53.401, P = .000, r = .541). The total expressive rate of GPX1 was 100% (20/20) in the well-differentiated group, 92.9% (39/42) in the moderately differentiated group and 66.3% (73/110) in the poorly differentiated group. The positive rate of GPX1 was associated with Lauren type (χ2 = 22.389, P = .000, r = .308). In 64 cases of intestinal type, the total positive rate was 92.2% (59/64), which was higher than that in the diffuse (61.4%, 43/70) and mixed types (78.9%, 30/38) (Table 1). The expression of GPX1 was associated with WHO type. The expression of GPX1 in the tubular type was stronger than that in the other types (χ2 = 28.081, P = .000, r = .396). In 142 cases of tubular type, 36 cases were scored as 2, and 23 were scored as 1. In 30 cases of other types, no cases were scored as 2, and 13 were scored as 1. The expression of GPX1 was negatively associated with lymph node involvement (χ2 = 9.710, P = .008, r = −.230). Out of 35 cases with no lymph node involvement, 12 cases were scored as 2, 19 were scored as 1, and 4 were scored as 0. Out of 42 cases with one lymph node involved, 10 cases were scored as 2, 25 were scored as 1, and 7 were scored as 0. Out of 95 cases with more than one lymph node involved, 14 cases were scored as 2, 52 were scored as 1, and 29 were scored as 0. The expression GPX1 was not associated with age, sex, or TNM stage (P > .05) (Table 1).


Significance of glutathione peroxidase 1 and caudal-related homeodomain transcription factor in human gastric adenocarcinoma.

Han JJ, Xie de R, Wang LL, Liu YQ, Wu GF, Sun Q, Chen YX, Wei Y, Huang ZQ, Li HG - Gastroenterol Res Pract (2013)

Immunohistochemical staining in GAC and ANM tissue. (a) GPX1 expression in ANM tissue (score 3). (b) GPX1 expression in well-differentiated GAC (score 3). (c) GPX1 expression in well-differentiated GAC (score 2). (d) GPX1 expression in signet-ring cell GAC (score 0). (e) CDX2 expression in ANM tissue (negative). ((f) and (g)) CDX2 expression in well-differentiated GAC (positive). (h) CDX2 expression in signet-ring cell GAC (negative). (i) CDX2 expression in normal colon mucosa (positive). ((j) and (k)) Ki67 expression in well-differentiated GAC (negative). (l) Ki67 expression in signet-ring cell GAC (negative). Original magnification: ×200.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig1: Immunohistochemical staining in GAC and ANM tissue. (a) GPX1 expression in ANM tissue (score 3). (b) GPX1 expression in well-differentiated GAC (score 3). (c) GPX1 expression in well-differentiated GAC (score 2). (d) GPX1 expression in signet-ring cell GAC (score 0). (e) CDX2 expression in ANM tissue (negative). ((f) and (g)) CDX2 expression in well-differentiated GAC (positive). (h) CDX2 expression in signet-ring cell GAC (negative). (i) CDX2 expression in normal colon mucosa (positive). ((j) and (k)) Ki67 expression in well-differentiated GAC (negative). (l) Ki67 expression in signet-ring cell GAC (negative). Original magnification: ×200.
Mentions: Out of 172 gastric adenocarcinomas, 1 case (0.6%) had an index score of 3 (the case scored as a 3 was merged with those cases scored as a 2, statistically), 35 cases (20.3%) had index scores of 2, 96 cases (55.8%) had index scores of 1, and 40 cases (23.3%) had index scores of 0 (Figures 1(a)–1(d)). The total positive rate of GPX1 in all 172 cases of GAC was 76.7% (132/172). All 16 of the ANM tissues samples were scored as a 3. The expression of GPX1 in ANM tissues was significantly stronger than that in GAC (Z = −7.170, P = .000, r = .664). The higher positive rate of GPX1 was associated with better differentiation (χ2 = 53.401, P = .000, r = .541). The total expressive rate of GPX1 was 100% (20/20) in the well-differentiated group, 92.9% (39/42) in the moderately differentiated group and 66.3% (73/110) in the poorly differentiated group. The positive rate of GPX1 was associated with Lauren type (χ2 = 22.389, P = .000, r = .308). In 64 cases of intestinal type, the total positive rate was 92.2% (59/64), which was higher than that in the diffuse (61.4%, 43/70) and mixed types (78.9%, 30/38) (Table 1). The expression of GPX1 was associated with WHO type. The expression of GPX1 in the tubular type was stronger than that in the other types (χ2 = 28.081, P = .000, r = .396). In 142 cases of tubular type, 36 cases were scored as 2, and 23 were scored as 1. In 30 cases of other types, no cases were scored as 2, and 13 were scored as 1. The expression of GPX1 was negatively associated with lymph node involvement (χ2 = 9.710, P = .008, r = −.230). Out of 35 cases with no lymph node involvement, 12 cases were scored as 2, 19 were scored as 1, and 4 were scored as 0. Out of 42 cases with one lymph node involved, 10 cases were scored as 2, 25 were scored as 1, and 7 were scored as 0. Out of 95 cases with more than one lymph node involved, 14 cases were scored as 2, 52 were scored as 1, and 29 were scored as 0. The expression GPX1 was not associated with age, sex, or TNM stage (P > .05) (Table 1).

Bottom Line: High expression of CDX2 was associated with differentiation, Lauren type, WHO type, extensive lymph node metastasis, and TNM of GAC.According to multivariate analysis, only lymph node metastasis, TNM stage, and CDX2 expression were independently associated with survival.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Zengcheng People's Hospital, Zengcheng City 511300, China ; Department of Pathology, Sun Yat-Sen Memorial Hospital, Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangdong Higher Education Institutes, Sun Yat-Sen University, Guangzhou 510120, China.

ABSTRACT
Aim. To investigate the expressions of glutathione peroxidase 1 (GPX1) and caudal-related homeodomain transcription factor (CDX2) in GAC and their correlation with clinicopathological features and tumor cell proliferation. Methods. The expressions of GPX1, CDX2, and Ki67 were immunohistochemically evaluated in 172 GAC specimens. The association of GPX1 and CDX2 with patient's clinicopathological features and Ki67 positive rate was analyzed statistically. Results. In 172 cases of GAC, the expression of GPX1 was weaker than that in adjacent normal mucosa, and the expression of CDX2 was higher than that in adjacent normal mucosa. High expression GPX1 strong-expression was associated with differentiation, Lauren type, WHO type and extensive lymph node metastasis of GAC. High expression of CDX2 was associated with differentiation, Lauren type, WHO type, extensive lymph node metastasis, and TNM of GAC. Survival curves showed that expressions of GPX1 and CDX2 were factors of good outcome (P = .03 and .02, resp.). According to multivariate analysis, only lymph node metastasis, TNM stage, and CDX2 expression were independently associated with survival. In addition, a strong association of GPX1 expression was noted with Ki67 and CDX2. Conclusions. The expression of GPX1 and CDX2 may play a role in the carcinogenesis, differentiation, and progression of GAC, and CDX2 may be an independent prognostic factor.

No MeSH data available.


Related in: MedlinePlus