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Network-based biomarkers for cold coagulation blood stasis syndrome and the therapeutic effects of shaofu zhuyu decoction in rats.

Su S, Duan J, Cui W, Shang E, Liu P, Bai G, Guo S, Qian D, Tang Y - Evid Based Complement Alternat Med (2013)

Bottom Line: Furthermore, the relative quantities of 6 endogenous metabolites in the plasma and 5 in the urine were significantly affected by SFZYD (P < 0.05).The pharmacological mechanism of SFZYD was partially associated with glycerophospholipid metabolism and pentose and glucuronate interconversions.In conclusion, our findings demonstrated that TCM CCBS pattern induced by ice water and epinephrine was complex and related to multiple metabolic pathways.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China.

ABSTRACT
In this study, the reverse docking methodology was applied to predict the action targets and pathways of Shaofu Zhuyu decoction (SFZYD) bioactive ingredients. Furthermore, Traditional Chinese Medicine (TCM) cold coagulation blood stasis (CCBS) syndrome was induced in female Sprague-Dawley rats with an ice-water bath and epinephrine, and SFZYD was used to treat CCBS syndrome. A metabolomic approach was used to evaluate changes in the metabolic profiles and to analyze the pharmacological mechanism of SFZYD actions. Twenty-three potential protein targets and 15 pathways were discovered, respectively; among these, pathways are associated with inflammation and immunological stress, hormone metabolism, coagulation function, and glycometabolism. There were also changes in the levels of endogenous metabolites of LysoPCs and glucuronides. Twenty endogenous metabolites were identified. Furthermore, the relative quantities of 6 endogenous metabolites in the plasma and 5 in the urine were significantly affected by SFZYD (P < 0.05). The pharmacological mechanism of SFZYD was partially associated with glycerophospholipid metabolism and pentose and glucuronate interconversions. In conclusion, our findings demonstrated that TCM CCBS pattern induced by ice water and epinephrine was complex and related to multiple metabolic pathways. SFZYD did regulate the TCM CCBS by multitargets, and biomarkers and SFZYD should be used for the clinical treatment of CCBS syndrome.

No MeSH data available.


Related in: MedlinePlus

S-plot of the OPLS-DA model for the plasma and urine samples from the normal versus model groups (plasma: (a) ES+ mode; (b) ESI− mode; urine: (c) ES+ mode; and (d) ES− mode).
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fig5: S-plot of the OPLS-DA model for the plasma and urine samples from the normal versus model groups (plasma: (a) ES+ mode; (b) ESI− mode; urine: (c) ES+ mode; and (d) ES− mode).

Mentions: Typical base peak intensity (BPI) chromatograms in positive and negative ion modes of plasma and urine samples collected from normal and model rats are shown in Figure S3. The unsupervised PCA model was used to separate the plasma and urine samples into two blocks, respectively, between the normal group and the model group in positive and negative ion modes (Figures 3(a), 3(b), 3(c), 3(d), 4(a), 4(b), 4(c), and 4(d)). The supervised OPLS-DA, which could improve biomarker discovery and separate the samples into two blocks, was applied to obtain better discrimination between the two groups. The OPLS-DA score plot analysis of the chromatographic data identified the plasma and urine samples of the normal group and the model group based on the differences in their metabolic profiles, which suggested that the metabolic profiles were significantly changed as a result of CCBS syndrome (Figures 5(a), 5(b), 5(c), and 5(d)). The recognition of a different pattern indicates that the endogenous metabolites have changed in CCBS syndrome model rats.


Network-based biomarkers for cold coagulation blood stasis syndrome and the therapeutic effects of shaofu zhuyu decoction in rats.

Su S, Duan J, Cui W, Shang E, Liu P, Bai G, Guo S, Qian D, Tang Y - Evid Based Complement Alternat Med (2013)

S-plot of the OPLS-DA model for the plasma and urine samples from the normal versus model groups (plasma: (a) ES+ mode; (b) ESI− mode; urine: (c) ES+ mode; and (d) ES− mode).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818846&req=5

fig5: S-plot of the OPLS-DA model for the plasma and urine samples from the normal versus model groups (plasma: (a) ES+ mode; (b) ESI− mode; urine: (c) ES+ mode; and (d) ES− mode).
Mentions: Typical base peak intensity (BPI) chromatograms in positive and negative ion modes of plasma and urine samples collected from normal and model rats are shown in Figure S3. The unsupervised PCA model was used to separate the plasma and urine samples into two blocks, respectively, between the normal group and the model group in positive and negative ion modes (Figures 3(a), 3(b), 3(c), 3(d), 4(a), 4(b), 4(c), and 4(d)). The supervised OPLS-DA, which could improve biomarker discovery and separate the samples into two blocks, was applied to obtain better discrimination between the two groups. The OPLS-DA score plot analysis of the chromatographic data identified the plasma and urine samples of the normal group and the model group based on the differences in their metabolic profiles, which suggested that the metabolic profiles were significantly changed as a result of CCBS syndrome (Figures 5(a), 5(b), 5(c), and 5(d)). The recognition of a different pattern indicates that the endogenous metabolites have changed in CCBS syndrome model rats.

Bottom Line: Furthermore, the relative quantities of 6 endogenous metabolites in the plasma and 5 in the urine were significantly affected by SFZYD (P < 0.05).The pharmacological mechanism of SFZYD was partially associated with glycerophospholipid metabolism and pentose and glucuronate interconversions.In conclusion, our findings demonstrated that TCM CCBS pattern induced by ice water and epinephrine was complex and related to multiple metabolic pathways.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China.

ABSTRACT
In this study, the reverse docking methodology was applied to predict the action targets and pathways of Shaofu Zhuyu decoction (SFZYD) bioactive ingredients. Furthermore, Traditional Chinese Medicine (TCM) cold coagulation blood stasis (CCBS) syndrome was induced in female Sprague-Dawley rats with an ice-water bath and epinephrine, and SFZYD was used to treat CCBS syndrome. A metabolomic approach was used to evaluate changes in the metabolic profiles and to analyze the pharmacological mechanism of SFZYD actions. Twenty-three potential protein targets and 15 pathways were discovered, respectively; among these, pathways are associated with inflammation and immunological stress, hormone metabolism, coagulation function, and glycometabolism. There were also changes in the levels of endogenous metabolites of LysoPCs and glucuronides. Twenty endogenous metabolites were identified. Furthermore, the relative quantities of 6 endogenous metabolites in the plasma and 5 in the urine were significantly affected by SFZYD (P < 0.05). The pharmacological mechanism of SFZYD was partially associated with glycerophospholipid metabolism and pentose and glucuronate interconversions. In conclusion, our findings demonstrated that TCM CCBS pattern induced by ice water and epinephrine was complex and related to multiple metabolic pathways. SFZYD did regulate the TCM CCBS by multitargets, and biomarkers and SFZYD should be used for the clinical treatment of CCBS syndrome.

No MeSH data available.


Related in: MedlinePlus