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The effect of electroacupuncture on osteosarcoma tumor growth and metastasis: analysis of different treatment regimens.

Smeester BA, Al-Gizawiy M, O'Brien EE, Ericson ME, Triemstra JL, Beitz AJ - Evid Based Complement Alternat Med (2013)

Bottom Line: We further demonstrate that EA-2X/3 treatment elicits significant reductions in tumor lymphatics, vasculature, and innervation.Lastly, EA-2X/3 treatment produced a marked reduction in pulmonary metastasis, thus providing evidence for EA's potential antimetastatic capabilities.Collectively, EA-2X/3 treatment was found to reduce both bone tumor growth and lung metastasis, which may be mediated in part through reductions in tumor-associated vasculature, lymphatics, and innervation.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA.

ABSTRACT
Osteosarcoma is the most common malignant bone tumor found in children and adolescents and is associated with many complications including cancer pain and metastasis. While cancer patients often seek complementary and alternative medicine (CAM) approaches to treat cancer pain and fatigue or the side effects of chemotherapy and treatment, there is little known about the effect of acupuncture treatment on tumor growth and metastasis. Here we evaluate the effects of six different electroacupuncture (EA) regimens on osteosarcoma tumor growth and metastasis in both male and female mice. The most significant positive effects were observed when EA was applied to the ST-36 acupoint twice weekly (EA-2X/3) beginning at postimplantation day 3 (PID 3). Twice weekly treatment produced robust reductions in tumor growth. Conversely, when EA was applied twice weekly (EA-2X/7), starting at PID 7, there was a significant increase in tumor growth. We further demonstrate that EA-2X/3 treatment elicits significant reductions in tumor lymphatics, vasculature, and innervation. Lastly, EA-2X/3 treatment produced a marked reduction in pulmonary metastasis, thus providing evidence for EA's potential antimetastatic capabilities. Collectively, EA-2X/3 treatment was found to reduce both bone tumor growth and lung metastasis, which may be mediated in part through reductions in tumor-associated vasculature, lymphatics, and innervation.

No MeSH data available.


Related in: MedlinePlus

Graphs illustrating the effects of electroacupuncture (EA) given twice weekly beginning PID 3 (EA-2X/3) on the average metastatic tumor area within the lung ((a) and (b); n = 3–6/group) and on the average lung area ((c) and (d); n = 3/group) at PID 40 (a) and PID 60 (b). The average metastatic tumor area was significantly reduced in the EA-2X/3 group on both PID 40 and PID 60. EA-2X/3 had no effect on the average lung area. Sections were stained by H&E, data shown as mean ± SEM; *P < 0.05 or **P < 0.01 as compared to Tumor-No EA control.
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fig6: Graphs illustrating the effects of electroacupuncture (EA) given twice weekly beginning PID 3 (EA-2X/3) on the average metastatic tumor area within the lung ((a) and (b); n = 3–6/group) and on the average lung area ((c) and (d); n = 3/group) at PID 40 (a) and PID 60 (b). The average metastatic tumor area was significantly reduced in the EA-2X/3 group on both PID 40 and PID 60. EA-2X/3 had no effect on the average lung area. Sections were stained by H&E, data shown as mean ± SEM; *P < 0.05 or **P < 0.01 as compared to Tumor-No EA control.

Mentions: A significant reduction in average lung tumor area was observed as early as PID 40 and was sustained to PID 60 when compared to the Tumor-No EA control group (Figures 6(a) and 6(b); *P < 0.05). Image analysis measurements for average tumor area on PID 40 were 367495 ± 86796 versus 28922 ± 4908; on PID 60 they were 415774 ± 136735 versus 76602 ± 22821, M tumor versus M EA/2X with EA-2X/3, respectively. This suggests that EA-2X/3 treatment reduces tumor metastases during both the early and later stages of tumor development.


The effect of electroacupuncture on osteosarcoma tumor growth and metastasis: analysis of different treatment regimens.

Smeester BA, Al-Gizawiy M, O'Brien EE, Ericson ME, Triemstra JL, Beitz AJ - Evid Based Complement Alternat Med (2013)

Graphs illustrating the effects of electroacupuncture (EA) given twice weekly beginning PID 3 (EA-2X/3) on the average metastatic tumor area within the lung ((a) and (b); n = 3–6/group) and on the average lung area ((c) and (d); n = 3/group) at PID 40 (a) and PID 60 (b). The average metastatic tumor area was significantly reduced in the EA-2X/3 group on both PID 40 and PID 60. EA-2X/3 had no effect on the average lung area. Sections were stained by H&E, data shown as mean ± SEM; *P < 0.05 or **P < 0.01 as compared to Tumor-No EA control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818845&req=5

fig6: Graphs illustrating the effects of electroacupuncture (EA) given twice weekly beginning PID 3 (EA-2X/3) on the average metastatic tumor area within the lung ((a) and (b); n = 3–6/group) and on the average lung area ((c) and (d); n = 3/group) at PID 40 (a) and PID 60 (b). The average metastatic tumor area was significantly reduced in the EA-2X/3 group on both PID 40 and PID 60. EA-2X/3 had no effect on the average lung area. Sections were stained by H&E, data shown as mean ± SEM; *P < 0.05 or **P < 0.01 as compared to Tumor-No EA control.
Mentions: A significant reduction in average lung tumor area was observed as early as PID 40 and was sustained to PID 60 when compared to the Tumor-No EA control group (Figures 6(a) and 6(b); *P < 0.05). Image analysis measurements for average tumor area on PID 40 were 367495 ± 86796 versus 28922 ± 4908; on PID 60 they were 415774 ± 136735 versus 76602 ± 22821, M tumor versus M EA/2X with EA-2X/3, respectively. This suggests that EA-2X/3 treatment reduces tumor metastases during both the early and later stages of tumor development.

Bottom Line: We further demonstrate that EA-2X/3 treatment elicits significant reductions in tumor lymphatics, vasculature, and innervation.Lastly, EA-2X/3 treatment produced a marked reduction in pulmonary metastasis, thus providing evidence for EA's potential antimetastatic capabilities.Collectively, EA-2X/3 treatment was found to reduce both bone tumor growth and lung metastasis, which may be mediated in part through reductions in tumor-associated vasculature, lymphatics, and innervation.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA.

ABSTRACT
Osteosarcoma is the most common malignant bone tumor found in children and adolescents and is associated with many complications including cancer pain and metastasis. While cancer patients often seek complementary and alternative medicine (CAM) approaches to treat cancer pain and fatigue or the side effects of chemotherapy and treatment, there is little known about the effect of acupuncture treatment on tumor growth and metastasis. Here we evaluate the effects of six different electroacupuncture (EA) regimens on osteosarcoma tumor growth and metastasis in both male and female mice. The most significant positive effects were observed when EA was applied to the ST-36 acupoint twice weekly (EA-2X/3) beginning at postimplantation day 3 (PID 3). Twice weekly treatment produced robust reductions in tumor growth. Conversely, when EA was applied twice weekly (EA-2X/7), starting at PID 7, there was a significant increase in tumor growth. We further demonstrate that EA-2X/3 treatment elicits significant reductions in tumor lymphatics, vasculature, and innervation. Lastly, EA-2X/3 treatment produced a marked reduction in pulmonary metastasis, thus providing evidence for EA's potential antimetastatic capabilities. Collectively, EA-2X/3 treatment was found to reduce both bone tumor growth and lung metastasis, which may be mediated in part through reductions in tumor-associated vasculature, lymphatics, and innervation.

No MeSH data available.


Related in: MedlinePlus