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Estimation of cutoff values of cotinine in urine and saliva for pregnant women in Poland.

Stragierowicz J, Mikołajewska K, Zawadzka-Stolarz M, Polańska K, Ligocka D - Biomed Res Int (2013)

Bottom Line: The cutoff values were established for saliva 12.9 ng/mL and urine 42.3 ng/mL or 53.1 μg/g creatinine with the ROC curve analysis.The developed analytical method was successfully applied to quantify cotinine, and a significant correlation between the urinary and salivary cotinine levels was found.The presented cut-off values for salivary and urinary cotinine ensure a categorization of the smoking status among pregnant women that is more accurate than self-reporting.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, 8 Teresy Street, 91-348 Lodz, Poland.

ABSTRACT
Setting appropriate cutoff values and the use of a highly sensitive analytical method allow for correct classification of the smoking status. Urine-saliva pairs samples of pregnant women in the second and third trimester, and saliva only in the first trimester were collected. Offline SPE and LC-ESI-MS/MS method was developed in the broad concentration range (saliva 0.4-1000 ng/mL, urine 0.8-4000 ng/mL). The mean recoveries were 3.7 ± 7.6% for urine and 99.1 ± 2.6% for saliva. LOD for saliva was 0.12 ng/mL and for urine 0.05 ng/mL; LOQ was 0.4 ng/mL and 0.8 ng/mL, respectively. Intraday and interday precision equaled, respectively, 1.2% and 3.4% for urine, and 2.3% and 6.4% for saliva. There was a strong correlation between salivary cotinine and the uncorrected cotinine concentration in urine in the second and third trimesters of pregnancy. The cutoff values were established for saliva 12.9 ng/mL and urine 42.3 ng/mL or 53.1 μg/g creatinine with the ROC curve analysis. The developed analytical method was successfully applied to quantify cotinine, and a significant correlation between the urinary and salivary cotinine levels was found. The presented cut-off values for salivary and urinary cotinine ensure a categorization of the smoking status among pregnant women that is more accurate than self-reporting.

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Comparison of the urinary and salivary cotinine concentrations, depending on the trimester of pregnancy and creatinine corrected concentration.
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fig1: Comparison of the urinary and salivary cotinine concentrations, depending on the trimester of pregnancy and creatinine corrected concentration.

Mentions: To assess the correlations between the matrices, the linear regression and Pearson's correlations were calculated. The results are shown in Figure 1. The correlations between the saliva and the uncorrected cotinine concentration in urine in trimester II (r = 0.932) and III (r = 0.925) were higher than the aggregated data of trimester II and III (r = 0.851), both being statistically significant (P < 0.01). Similar trends were observed for the correlation between saliva and creatinine-corrected cotinine in urine, but the corresponding r values were lower, that is, 0.720, 0.865, and 0.780, for trimester II, III, and the total period of pregnancy, respectively.


Estimation of cutoff values of cotinine in urine and saliva for pregnant women in Poland.

Stragierowicz J, Mikołajewska K, Zawadzka-Stolarz M, Polańska K, Ligocka D - Biomed Res Int (2013)

Comparison of the urinary and salivary cotinine concentrations, depending on the trimester of pregnancy and creatinine corrected concentration.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3818804&req=5

fig1: Comparison of the urinary and salivary cotinine concentrations, depending on the trimester of pregnancy and creatinine corrected concentration.
Mentions: To assess the correlations between the matrices, the linear regression and Pearson's correlations were calculated. The results are shown in Figure 1. The correlations between the saliva and the uncorrected cotinine concentration in urine in trimester II (r = 0.932) and III (r = 0.925) were higher than the aggregated data of trimester II and III (r = 0.851), both being statistically significant (P < 0.01). Similar trends were observed for the correlation between saliva and creatinine-corrected cotinine in urine, but the corresponding r values were lower, that is, 0.720, 0.865, and 0.780, for trimester II, III, and the total period of pregnancy, respectively.

Bottom Line: The cutoff values were established for saliva 12.9 ng/mL and urine 42.3 ng/mL or 53.1 μg/g creatinine with the ROC curve analysis.The developed analytical method was successfully applied to quantify cotinine, and a significant correlation between the urinary and salivary cotinine levels was found.The presented cut-off values for salivary and urinary cotinine ensure a categorization of the smoking status among pregnant women that is more accurate than self-reporting.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, 8 Teresy Street, 91-348 Lodz, Poland.

ABSTRACT
Setting appropriate cutoff values and the use of a highly sensitive analytical method allow for correct classification of the smoking status. Urine-saliva pairs samples of pregnant women in the second and third trimester, and saliva only in the first trimester were collected. Offline SPE and LC-ESI-MS/MS method was developed in the broad concentration range (saliva 0.4-1000 ng/mL, urine 0.8-4000 ng/mL). The mean recoveries were 3.7 ± 7.6% for urine and 99.1 ± 2.6% for saliva. LOD for saliva was 0.12 ng/mL and for urine 0.05 ng/mL; LOQ was 0.4 ng/mL and 0.8 ng/mL, respectively. Intraday and interday precision equaled, respectively, 1.2% and 3.4% for urine, and 2.3% and 6.4% for saliva. There was a strong correlation between salivary cotinine and the uncorrected cotinine concentration in urine in the second and third trimesters of pregnancy. The cutoff values were established for saliva 12.9 ng/mL and urine 42.3 ng/mL or 53.1 μg/g creatinine with the ROC curve analysis. The developed analytical method was successfully applied to quantify cotinine, and a significant correlation between the urinary and salivary cotinine levels was found. The presented cut-off values for salivary and urinary cotinine ensure a categorization of the smoking status among pregnant women that is more accurate than self-reporting.

Show MeSH