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Association of 3-Dimensional Cartilage and Bone Structure with Articular Cartilage Properties in and Adjacent to Autologous Osteochondral Grafts after 6 and 12 months in a Goat Model.

Chan EF, Liu IL, Semler EJ, Aberman HM, Simon TM, Chen AC, Truncale KG, Sah RL - Cartilage (2012)

Bottom Line: Operated MFC articular surfaces were recessed relative to Non-Operated MFC and exhibited lower cartilage stiffness with increasing recession.Sites with large bone-cartilage interface deviations, both proud and recessed, were associated with recessed articular surfaces and low cartilage stiffness.The effectiveness of cartilage repair by osteochondral grafting is associated with the match of 3-D cartilage and bone geometry to the native osteochondral structure.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of California, San Diego.

ABSTRACT

Objective: The articular cartilage of autologous osteochondral grafts is typically different in structure and function from local host cartilage and thereby presents a remodeling challenge. The hypothesis of this study was that properties of the articular cartilage of trochlear autografts and adjacent femoral condyle are associated with the 3-D geometrical match between grafted and contralateral joints at 6 and 12 months after surgery.

Design: Autografts were transferred unilaterally from the lateral trochlea (LT) to the medial femoral condyle (MFC) in adult Spanish goats. Operated and contralateral Non-Operated joints were harvested at 6 and 12 months, and analyzed by indentation testing, micro-computed tomography, and histology to compare (1) histological indices of repair, (2) 3-D structure (articular surface deviation, bone-cartilage interface deviation, cartilage thickness), (3) indentation stiffness, and (4) correlations between stiffness and 3-D structure.

Results: Cartilage deterioration was present in grafts at 6 months and more severe at 12 months. Cartilage thickness and normalized stiffness of Operated MFC were lower than Non-Operated MFC within the graft and proximal adjacent host regions. Operated MFC articular surfaces were recessed relative to Non-Operated MFC and exhibited lower cartilage stiffness with increasing recession. Sites with large bone-cartilage interface deviations, both proud and recessed, were associated with recessed articular surfaces and low cartilage stiffness.

Conclusion: The effectiveness of cartilage repair by osteochondral grafting is associated with the match of 3-D cartilage and bone geometry to the native osteochondral structure.

No MeSH data available.


Related in: MedlinePlus

(A) In operated knee joints, osteochondral autografts 3.5 mm in diameter were obtained from the lateral trochlea (LT) and transplanted into the medial femoral condyle (MFC). (B) Both operated and contralateral nonoperated joints were analyzed at and adjacent to the graft region by indentation testing in an array of 63 positions along central (C), medial (M), and lateral (L) paths oriented proximally-distally. Positions were classified as graft, proximal adjacent host (PAHC), or distal adjacent host (DAHC) regions. (C) Operated and nonoperated joint MFC and LT regions (boxed) were then analyzed with micro–computed tomography, histology, and immunohistochemistry.
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fig1-1947603511435272: (A) In operated knee joints, osteochondral autografts 3.5 mm in diameter were obtained from the lateral trochlea (LT) and transplanted into the medial femoral condyle (MFC). (B) Both operated and contralateral nonoperated joints were analyzed at and adjacent to the graft region by indentation testing in an array of 63 positions along central (C), medial (M), and lateral (L) paths oriented proximally-distally. Positions were classified as graft, proximal adjacent host (PAHC), or distal adjacent host (DAHC) regions. (C) Operated and nonoperated joint MFC and LT regions (boxed) were then analyzed with micro–computed tomography, histology, and immunohistochemistry.

Mentions: Adult female Spanish goats (2-3 years old) were used with UCSD Institutional Animal Care and Use Committee approval. In the operated knee of each goat, a full-thickness osteochondral graft (diameter [Ø] = 3.5 mm, height [h] = 6 mm) was harvested from the LT using a trephine (Smith & Nephew, Andover, MA) and press-fit into recipient osteochondral defects (Ø = 3.5 mm) drilled in the weightbearing surface of the MFC (see supplementary material). Care was taken to ensure that the graft articular surfaces were approximately flush with host articular surfaces. At 6 and 12 months (n = 4 each), animals were euthanized, and both operated and contralateral nonoperated knees were harvested for analysis (Fig. 1).


Association of 3-Dimensional Cartilage and Bone Structure with Articular Cartilage Properties in and Adjacent to Autologous Osteochondral Grafts after 6 and 12 months in a Goat Model.

Chan EF, Liu IL, Semler EJ, Aberman HM, Simon TM, Chen AC, Truncale KG, Sah RL - Cartilage (2012)

(A) In operated knee joints, osteochondral autografts 3.5 mm in diameter were obtained from the lateral trochlea (LT) and transplanted into the medial femoral condyle (MFC). (B) Both operated and contralateral nonoperated joints were analyzed at and adjacent to the graft region by indentation testing in an array of 63 positions along central (C), medial (M), and lateral (L) paths oriented proximally-distally. Positions were classified as graft, proximal adjacent host (PAHC), or distal adjacent host (DAHC) regions. (C) Operated and nonoperated joint MFC and LT regions (boxed) were then analyzed with micro–computed tomography, histology, and immunohistochemistry.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818730&req=5

fig1-1947603511435272: (A) In operated knee joints, osteochondral autografts 3.5 mm in diameter were obtained from the lateral trochlea (LT) and transplanted into the medial femoral condyle (MFC). (B) Both operated and contralateral nonoperated joints were analyzed at and adjacent to the graft region by indentation testing in an array of 63 positions along central (C), medial (M), and lateral (L) paths oriented proximally-distally. Positions were classified as graft, proximal adjacent host (PAHC), or distal adjacent host (DAHC) regions. (C) Operated and nonoperated joint MFC and LT regions (boxed) were then analyzed with micro–computed tomography, histology, and immunohistochemistry.
Mentions: Adult female Spanish goats (2-3 years old) were used with UCSD Institutional Animal Care and Use Committee approval. In the operated knee of each goat, a full-thickness osteochondral graft (diameter [Ø] = 3.5 mm, height [h] = 6 mm) was harvested from the LT using a trephine (Smith & Nephew, Andover, MA) and press-fit into recipient osteochondral defects (Ø = 3.5 mm) drilled in the weightbearing surface of the MFC (see supplementary material). Care was taken to ensure that the graft articular surfaces were approximately flush with host articular surfaces. At 6 and 12 months (n = 4 each), animals were euthanized, and both operated and contralateral nonoperated knees were harvested for analysis (Fig. 1).

Bottom Line: Operated MFC articular surfaces were recessed relative to Non-Operated MFC and exhibited lower cartilage stiffness with increasing recession.Sites with large bone-cartilage interface deviations, both proud and recessed, were associated with recessed articular surfaces and low cartilage stiffness.The effectiveness of cartilage repair by osteochondral grafting is associated with the match of 3-D cartilage and bone geometry to the native osteochondral structure.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of California, San Diego.

ABSTRACT

Objective: The articular cartilage of autologous osteochondral grafts is typically different in structure and function from local host cartilage and thereby presents a remodeling challenge. The hypothesis of this study was that properties of the articular cartilage of trochlear autografts and adjacent femoral condyle are associated with the 3-D geometrical match between grafted and contralateral joints at 6 and 12 months after surgery.

Design: Autografts were transferred unilaterally from the lateral trochlea (LT) to the medial femoral condyle (MFC) in adult Spanish goats. Operated and contralateral Non-Operated joints were harvested at 6 and 12 months, and analyzed by indentation testing, micro-computed tomography, and histology to compare (1) histological indices of repair, (2) 3-D structure (articular surface deviation, bone-cartilage interface deviation, cartilage thickness), (3) indentation stiffness, and (4) correlations between stiffness and 3-D structure.

Results: Cartilage deterioration was present in grafts at 6 months and more severe at 12 months. Cartilage thickness and normalized stiffness of Operated MFC were lower than Non-Operated MFC within the graft and proximal adjacent host regions. Operated MFC articular surfaces were recessed relative to Non-Operated MFC and exhibited lower cartilage stiffness with increasing recession. Sites with large bone-cartilage interface deviations, both proud and recessed, were associated with recessed articular surfaces and low cartilage stiffness.

Conclusion: The effectiveness of cartilage repair by osteochondral grafting is associated with the match of 3-D cartilage and bone geometry to the native osteochondral structure.

No MeSH data available.


Related in: MedlinePlus