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Lack of in vitro effect of aglepristone on IFN-γ and IL-4 production by resting and mitogen-activated T cells of luteal bitches.

Jurka P, Szulc-Dąbrowska L, Borkowska J, Winnicka A - BMC Vet. Res. (2013)

Bottom Line: Our results showed no statistically significant differences in the percentage of IFN-γ and IL-4-synthesizing CD4+ or CD8+ resting T cells between untreated and aglepristone-treated cells at 24 and 48 hours post treatment.Presented results indicate that administration of aglepristone for 48 hours has no influence on IFN-γ and IL-4 synthesis by resting and mitogen-activated T cells isolated from diestral bitches.We conclude that antiprogestins may differentially affect T cell function depending on the animal species in which they are applied.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Small Animal Diseases with Clinic, Laboratory of Small Animal Reproduction, Warsaw, Poland. piotr_jurka@sggw.pl.

ABSTRACT

Background: Aglepristone (RU534) is an antiprogestin used for pregnancy termination, parturition induction and conservative pyometra treatment in bitches. Its molecular structure is similar to mifepristone, an antiprogestin used in human medicine. Mifepristone has been shown to suppress proliferation and cytokine production by T cells, whereas the effect of aglepristone on T cell function remains elusive. The purpose of this project was to investigate the in vitro influence of RU534 on IFN-γ and IL-4 synthesis by peripheral blood T cells isolated from healthy bitches (N = 16) in luteal phase. The peripheral blood mononuclear cells (PBMCs) were incubated with three different dosages of aglepristone, or dimethyl sulfoxide (DMSO), with or without mitogen. The production of cytokines by resting or mitogen-activated T cells was determined by intercellular staining and flow cytometry analysis or ELISA assay, respectively.

Results: Our results showed no statistically significant differences in the percentage of IFN-γ and IL-4-synthesizing CD4+ or CD8+ resting T cells between untreated and aglepristone-treated cells at 24 and 48 hours post treatment. Moreover, mitogen-activated PBMCs treated with RU534 displayed similar concentration of IFN-γ and IL-4 in culture supernatants to those observed in mitogen-activated DMSO-treated PBMCs. Presented results indicate that administration of aglepristone for 48 hours has no influence on IFN-γ and IL-4 synthesis by resting and mitogen-activated T cells isolated from diestral bitches.

Conclusions: We conclude that antiprogestins may differentially affect T cell function depending on the animal species in which they are applied.

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Related in: MedlinePlus

Representative dot plots show synthesis of IL-4 by CD4+ (A) and CD8+ (B) T cells isolated form bitches in luteal phase and treated for 24 and 48 h with DMSO (control) or 30, 300 and 3000 ng/ml of aglepristone. Numbers within quadrants represent the percentage of positive cells for a given marker within the gate for lymphocytes. MFI of IL-4 in T cell subsets is shown within upper right quadrants.
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Figure 3: Representative dot plots show synthesis of IL-4 by CD4+ (A) and CD8+ (B) T cells isolated form bitches in luteal phase and treated for 24 and 48 h with DMSO (control) or 30, 300 and 3000 ng/ml of aglepristone. Numbers within quadrants represent the percentage of positive cells for a given marker within the gate for lymphocytes. MFI of IL-4 in T cell subsets is shown within upper right quadrants.

Mentions: The percentage of both CD4+ and CD8+ T cells-synthesizing IL-4 ranged from around 1% to around 4% in control group and groups treated with different dosages of aglepristone (Figure 3). Our results indicated no statistically significant changes in the percentage of IL-4-synthesizing CD4+ and CD8+ T cells in group treated with aglepristone compared to control group (Figure 4A). Moreover, in both T cell subsets no statistically significant changes in MFI of IL-4 expression were observed between control and studied groups (Figure 4B).


Lack of in vitro effect of aglepristone on IFN-γ and IL-4 production by resting and mitogen-activated T cells of luteal bitches.

Jurka P, Szulc-Dąbrowska L, Borkowska J, Winnicka A - BMC Vet. Res. (2013)

Representative dot plots show synthesis of IL-4 by CD4+ (A) and CD8+ (B) T cells isolated form bitches in luteal phase and treated for 24 and 48 h with DMSO (control) or 30, 300 and 3000 ng/ml of aglepristone. Numbers within quadrants represent the percentage of positive cells for a given marker within the gate for lymphocytes. MFI of IL-4 in T cell subsets is shown within upper right quadrants.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3818567&req=5

Figure 3: Representative dot plots show synthesis of IL-4 by CD4+ (A) and CD8+ (B) T cells isolated form bitches in luteal phase and treated for 24 and 48 h with DMSO (control) or 30, 300 and 3000 ng/ml of aglepristone. Numbers within quadrants represent the percentage of positive cells for a given marker within the gate for lymphocytes. MFI of IL-4 in T cell subsets is shown within upper right quadrants.
Mentions: The percentage of both CD4+ and CD8+ T cells-synthesizing IL-4 ranged from around 1% to around 4% in control group and groups treated with different dosages of aglepristone (Figure 3). Our results indicated no statistically significant changes in the percentage of IL-4-synthesizing CD4+ and CD8+ T cells in group treated with aglepristone compared to control group (Figure 4A). Moreover, in both T cell subsets no statistically significant changes in MFI of IL-4 expression were observed between control and studied groups (Figure 4B).

Bottom Line: Our results showed no statistically significant differences in the percentage of IFN-γ and IL-4-synthesizing CD4+ or CD8+ resting T cells between untreated and aglepristone-treated cells at 24 and 48 hours post treatment.Presented results indicate that administration of aglepristone for 48 hours has no influence on IFN-γ and IL-4 synthesis by resting and mitogen-activated T cells isolated from diestral bitches.We conclude that antiprogestins may differentially affect T cell function depending on the animal species in which they are applied.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Small Animal Diseases with Clinic, Laboratory of Small Animal Reproduction, Warsaw, Poland. piotr_jurka@sggw.pl.

ABSTRACT

Background: Aglepristone (RU534) is an antiprogestin used for pregnancy termination, parturition induction and conservative pyometra treatment in bitches. Its molecular structure is similar to mifepristone, an antiprogestin used in human medicine. Mifepristone has been shown to suppress proliferation and cytokine production by T cells, whereas the effect of aglepristone on T cell function remains elusive. The purpose of this project was to investigate the in vitro influence of RU534 on IFN-γ and IL-4 synthesis by peripheral blood T cells isolated from healthy bitches (N = 16) in luteal phase. The peripheral blood mononuclear cells (PBMCs) were incubated with three different dosages of aglepristone, or dimethyl sulfoxide (DMSO), with or without mitogen. The production of cytokines by resting or mitogen-activated T cells was determined by intercellular staining and flow cytometry analysis or ELISA assay, respectively.

Results: Our results showed no statistically significant differences in the percentage of IFN-γ and IL-4-synthesizing CD4+ or CD8+ resting T cells between untreated and aglepristone-treated cells at 24 and 48 hours post treatment. Moreover, mitogen-activated PBMCs treated with RU534 displayed similar concentration of IFN-γ and IL-4 in culture supernatants to those observed in mitogen-activated DMSO-treated PBMCs. Presented results indicate that administration of aglepristone for 48 hours has no influence on IFN-γ and IL-4 synthesis by resting and mitogen-activated T cells isolated from diestral bitches.

Conclusions: We conclude that antiprogestins may differentially affect T cell function depending on the animal species in which they are applied.

Show MeSH
Related in: MedlinePlus