Limits...
Distinct phyllosphere bacterial communities on Arabidopsis wax mutant leaves.

Reisberg EE, Hildebrandt U, Riederer M, Hentschel U - PLoS ONE (2013)

Bottom Line: The chemical distinctness of the mutant wax phenotypes was confirmed by gas chromatographic measurements.Microbial community analyses revealed bacterial phylotypes that were ubiquitously present on all plant lines (termed "core" community) while others were positively or negatively affected by the wax mutant phenotype (termed "plant line-specific" community).We conclude from this study that plant cuticular wax composition can affect the community composition of phyllosphere bacteria.

View Article: PubMed Central - PubMed

Affiliation: University of Würzburg, Department of Botany II, Julius-von-Sachs-Institute for Biological Sciences, Würzburg, Germany.

ABSTRACT
The phyllosphere of plants is inhabited by diverse microorganisms, however, the factors shaping their community composition are not fully elucidated. The plant cuticle represents the initial contact surface between microorganisms and the plant. We thus aimed to investigate whether mutations in the cuticular wax biosynthesis would affect the diversity of the phyllosphere microbiota. A set of four Arabidopsis thaliana eceriferum mutants (cer1, cer6, cer9, cer16) and their respective wild type (Landsberg erecta) were subjected to an outdoor growth period and analysed towards this purpose. The chemical distinctness of the mutant wax phenotypes was confirmed by gas chromatographic measurements. Next generation amplicon pyrosequencing of the bacterial communities showed distinct community patterns. This observation was supported by denaturing gradient gel electrophoresis experiments. Microbial community analyses revealed bacterial phylotypes that were ubiquitously present on all plant lines (termed "core" community) while others were positively or negatively affected by the wax mutant phenotype (termed "plant line-specific" community). We conclude from this study that plant cuticular wax composition can affect the community composition of phyllosphere bacteria.

Show MeSH
Venn diagram of the plant-line specific communities.Counts of uniquely present (Fig. 6A) or uniquely absent (Fig. 6B) phylotypes are shown. OTUs that were present or absent on multiple plant lines are indicated by numbers in the overlapping areas of the diagram. Grey writing indicates phylotypes, which were represented by sequence abundances below 1% of the plant line-specific communities. The plant line-specific community data was extracted from a rarified dataset and only those OTUs were included that were found non–chimeric with both (Greengenes and Silva_108) reference datasets. The closest possible taxonomic affiliation is given. OTU numbers are given in brackets.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3818481&req=5

pone-0078613-g006: Venn diagram of the plant-line specific communities.Counts of uniquely present (Fig. 6A) or uniquely absent (Fig. 6B) phylotypes are shown. OTUs that were present or absent on multiple plant lines are indicated by numbers in the overlapping areas of the diagram. Grey writing indicates phylotypes, which were represented by sequence abundances below 1% of the plant line-specific communities. The plant line-specific community data was extracted from a rarified dataset and only those OTUs were included that were found non–chimeric with both (Greengenes and Silva_108) reference datasets. The closest possible taxonomic affiliation is given. OTU numbers are given in brackets.

Mentions: We further elaborated the analysis of the “plant line-specific” community based on a rarefied dataset. This effort was undertaken in order to highlight the “plant line-specific” OTUs, which contributed most to the statistical differences between plant lines under the most stringent selection criteria (Tab.2). In Figure 6 we show those OTUs that are uniquely present (Fig.6B) or uniquely absent (Fig.6C) in the resident community of a given plant line as well as the respective major wax compositional changes (Fig.6A). In doing so, 5, 2, 7, and 5 uniquely present OTUs were identified in the resident communities of the mutants cer1, cer6, cer9, and cer16. When analysed for the absence of OTUs, the mutants were specifically missing 1 (cer1), 3 (cer6), 1 (cer9), and 2 (cer16) OTUs in comparison to the other plant lines.


Distinct phyllosphere bacterial communities on Arabidopsis wax mutant leaves.

Reisberg EE, Hildebrandt U, Riederer M, Hentschel U - PLoS ONE (2013)

Venn diagram of the plant-line specific communities.Counts of uniquely present (Fig. 6A) or uniquely absent (Fig. 6B) phylotypes are shown. OTUs that were present or absent on multiple plant lines are indicated by numbers in the overlapping areas of the diagram. Grey writing indicates phylotypes, which were represented by sequence abundances below 1% of the plant line-specific communities. The plant line-specific community data was extracted from a rarified dataset and only those OTUs were included that were found non–chimeric with both (Greengenes and Silva_108) reference datasets. The closest possible taxonomic affiliation is given. OTU numbers are given in brackets.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818481&req=5

pone-0078613-g006: Venn diagram of the plant-line specific communities.Counts of uniquely present (Fig. 6A) or uniquely absent (Fig. 6B) phylotypes are shown. OTUs that were present or absent on multiple plant lines are indicated by numbers in the overlapping areas of the diagram. Grey writing indicates phylotypes, which were represented by sequence abundances below 1% of the plant line-specific communities. The plant line-specific community data was extracted from a rarified dataset and only those OTUs were included that were found non–chimeric with both (Greengenes and Silva_108) reference datasets. The closest possible taxonomic affiliation is given. OTU numbers are given in brackets.
Mentions: We further elaborated the analysis of the “plant line-specific” community based on a rarefied dataset. This effort was undertaken in order to highlight the “plant line-specific” OTUs, which contributed most to the statistical differences between plant lines under the most stringent selection criteria (Tab.2). In Figure 6 we show those OTUs that are uniquely present (Fig.6B) or uniquely absent (Fig.6C) in the resident community of a given plant line as well as the respective major wax compositional changes (Fig.6A). In doing so, 5, 2, 7, and 5 uniquely present OTUs were identified in the resident communities of the mutants cer1, cer6, cer9, and cer16. When analysed for the absence of OTUs, the mutants were specifically missing 1 (cer1), 3 (cer6), 1 (cer9), and 2 (cer16) OTUs in comparison to the other plant lines.

Bottom Line: The chemical distinctness of the mutant wax phenotypes was confirmed by gas chromatographic measurements.Microbial community analyses revealed bacterial phylotypes that were ubiquitously present on all plant lines (termed "core" community) while others were positively or negatively affected by the wax mutant phenotype (termed "plant line-specific" community).We conclude from this study that plant cuticular wax composition can affect the community composition of phyllosphere bacteria.

View Article: PubMed Central - PubMed

Affiliation: University of Würzburg, Department of Botany II, Julius-von-Sachs-Institute for Biological Sciences, Würzburg, Germany.

ABSTRACT
The phyllosphere of plants is inhabited by diverse microorganisms, however, the factors shaping their community composition are not fully elucidated. The plant cuticle represents the initial contact surface between microorganisms and the plant. We thus aimed to investigate whether mutations in the cuticular wax biosynthesis would affect the diversity of the phyllosphere microbiota. A set of four Arabidopsis thaliana eceriferum mutants (cer1, cer6, cer9, cer16) and their respective wild type (Landsberg erecta) were subjected to an outdoor growth period and analysed towards this purpose. The chemical distinctness of the mutant wax phenotypes was confirmed by gas chromatographic measurements. Next generation amplicon pyrosequencing of the bacterial communities showed distinct community patterns. This observation was supported by denaturing gradient gel electrophoresis experiments. Microbial community analyses revealed bacterial phylotypes that were ubiquitously present on all plant lines (termed "core" community) while others were positively or negatively affected by the wax mutant phenotype (termed "plant line-specific" community). We conclude from this study that plant cuticular wax composition can affect the community composition of phyllosphere bacteria.

Show MeSH