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Astragalus saponins affect proliferation, invasion and apoptosis of gastric cancer BGC-823 cells.

Wang T, Xuan X, Li M, Gao P, Zheng Y, Zang W, Zhao G - Diagn Pathol (2013)

Bottom Line: Cells proliferation was determined by CCK-8 assay.Cell cycle and apoptosis were detected by the flow cytometry.Total Astragalus saponins inhibited human gastric cancer cell growth, decreased the invasion ability and induced the apoptosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China. zangwenqiao@sina.com.

ABSTRACT

Background: Astragalus memebranaceus is a traditional Chinese herbal medicine used in treatment of common cold, diarrhea, fatigue, anorexia and cardiac diseases. Recently, there are growing evidences that Astragalus extract may be a potential anti-tumorigenic agent. Some research showed that the total saponins obtained from Astragalus membranaceus possess significant antitumorigenic activity. Gastric cancer is one of the most frequent cancers in the world, almost two-thirds of gastric cancer cases and deaths occur in less developed regions. But the effect of Astragalus membranaceus on proliferation, invasion and apoptosis of gastric cancer BGC-823 cells remains unclear.

Methods: Astragalus saponins were extracted. Cells proliferation was determined by CCK-8 assay. Cell cycle and apoptosis were detected by the flow cytometry. Boyden chamber was used to evaluate the invasion and metastasis capabilities of BGC-823 cells. Tumor growth was assessed by subcutaneous inoculation of cells into BALB/c nude mice.

Results: The results demonstrated that total Astragalus saponins could inhibit human gastric cancer cell growth both in vitro and in vivo, in additional, Astragalus saponins deceased the invasion ability and induced the apoptosis of gastric cancer BGC-823 cells.

Conclusions: Total Astragalus saponins inhibited human gastric cancer cell growth, decreased the invasion ability and induced the apoptosis. This suggested the possibility of further developing Astragalus as an alternative treatment option, or perhaps using it as adjuvant chemotherapeutic agent in gastric cancer therapy.

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Related in: MedlinePlus

BGC-823 cell apoptosis and xenograft tumor experiment. A. Astragalus saponins induced apoptosis in BGC-823 cells. Cell apoptosis was analyzed by flow cytometry. Statistically significant (*p < 0.05)increases in annexin V + apoptotic cells were observed in Astragalus saponins–treated BGC-823 cells compared to controls. Data are presented as the mean of triplicate experiments. B. BGC-823 cell xenograft tumor experiment. Injection of Astragalus saponins inhibited tumor growth in a BGC-823 xenograft model compared with the blank control group. *Significant difference (p < 0.05).
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Figure 3: BGC-823 cell apoptosis and xenograft tumor experiment. A. Astragalus saponins induced apoptosis in BGC-823 cells. Cell apoptosis was analyzed by flow cytometry. Statistically significant (*p < 0.05)increases in annexin V + apoptotic cells were observed in Astragalus saponins–treated BGC-823 cells compared to controls. Data are presented as the mean of triplicate experiments. B. BGC-823 cell xenograft tumor experiment. Injection of Astragalus saponins inhibited tumor growth in a BGC-823 xenograft model compared with the blank control group. *Significant difference (p < 0.05).

Mentions: BGC-823 cells apoptosis was measured by flow cytometry. Statistically significant (p < 0.05)increases in annexin V + apoptotic cells were observed in 20 μg/ml(6.53 ± 0.62%), 40 μg/ml(12.14 ± 0.69%) and 80 μg/ml (18.2 ± 0.79%)Astragalus saponins–treated BGC-823 cell lines compared to control group (3.56 ± 0.45%) (Figure 3A). These results demonstrated that Astragalus saponins induced apoptosis of BGC-823 cells in vitro.


Astragalus saponins affect proliferation, invasion and apoptosis of gastric cancer BGC-823 cells.

Wang T, Xuan X, Li M, Gao P, Zheng Y, Zang W, Zhao G - Diagn Pathol (2013)

BGC-823 cell apoptosis and xenograft tumor experiment. A. Astragalus saponins induced apoptosis in BGC-823 cells. Cell apoptosis was analyzed by flow cytometry. Statistically significant (*p < 0.05)increases in annexin V + apoptotic cells were observed in Astragalus saponins–treated BGC-823 cells compared to controls. Data are presented as the mean of triplicate experiments. B. BGC-823 cell xenograft tumor experiment. Injection of Astragalus saponins inhibited tumor growth in a BGC-823 xenograft model compared with the blank control group. *Significant difference (p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3818446&req=5

Figure 3: BGC-823 cell apoptosis and xenograft tumor experiment. A. Astragalus saponins induced apoptosis in BGC-823 cells. Cell apoptosis was analyzed by flow cytometry. Statistically significant (*p < 0.05)increases in annexin V + apoptotic cells were observed in Astragalus saponins–treated BGC-823 cells compared to controls. Data are presented as the mean of triplicate experiments. B. BGC-823 cell xenograft tumor experiment. Injection of Astragalus saponins inhibited tumor growth in a BGC-823 xenograft model compared with the blank control group. *Significant difference (p < 0.05).
Mentions: BGC-823 cells apoptosis was measured by flow cytometry. Statistically significant (p < 0.05)increases in annexin V + apoptotic cells were observed in 20 μg/ml(6.53 ± 0.62%), 40 μg/ml(12.14 ± 0.69%) and 80 μg/ml (18.2 ± 0.79%)Astragalus saponins–treated BGC-823 cell lines compared to control group (3.56 ± 0.45%) (Figure 3A). These results demonstrated that Astragalus saponins induced apoptosis of BGC-823 cells in vitro.

Bottom Line: Cells proliferation was determined by CCK-8 assay.Cell cycle and apoptosis were detected by the flow cytometry.Total Astragalus saponins inhibited human gastric cancer cell growth, decreased the invasion ability and induced the apoptosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China. zangwenqiao@sina.com.

ABSTRACT

Background: Astragalus memebranaceus is a traditional Chinese herbal medicine used in treatment of common cold, diarrhea, fatigue, anorexia and cardiac diseases. Recently, there are growing evidences that Astragalus extract may be a potential anti-tumorigenic agent. Some research showed that the total saponins obtained from Astragalus membranaceus possess significant antitumorigenic activity. Gastric cancer is one of the most frequent cancers in the world, almost two-thirds of gastric cancer cases and deaths occur in less developed regions. But the effect of Astragalus membranaceus on proliferation, invasion and apoptosis of gastric cancer BGC-823 cells remains unclear.

Methods: Astragalus saponins were extracted. Cells proliferation was determined by CCK-8 assay. Cell cycle and apoptosis were detected by the flow cytometry. Boyden chamber was used to evaluate the invasion and metastasis capabilities of BGC-823 cells. Tumor growth was assessed by subcutaneous inoculation of cells into BALB/c nude mice.

Results: The results demonstrated that total Astragalus saponins could inhibit human gastric cancer cell growth both in vitro and in vivo, in additional, Astragalus saponins deceased the invasion ability and induced the apoptosis of gastric cancer BGC-823 cells.

Conclusions: Total Astragalus saponins inhibited human gastric cancer cell growth, decreased the invasion ability and induced the apoptosis. This suggested the possibility of further developing Astragalus as an alternative treatment option, or perhaps using it as adjuvant chemotherapeutic agent in gastric cancer therapy.

Show MeSH
Related in: MedlinePlus