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Astragalus saponins affect proliferation, invasion and apoptosis of gastric cancer BGC-823 cells.

Wang T, Xuan X, Li M, Gao P, Zheng Y, Zang W, Zhao G - Diagn Pathol (2013)

Bottom Line: Cells proliferation was determined by CCK-8 assay.Cell cycle and apoptosis were detected by the flow cytometry.Total Astragalus saponins inhibited human gastric cancer cell growth, decreased the invasion ability and induced the apoptosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China. zangwenqiao@sina.com.

ABSTRACT

Background: Astragalus memebranaceus is a traditional Chinese herbal medicine used in treatment of common cold, diarrhea, fatigue, anorexia and cardiac diseases. Recently, there are growing evidences that Astragalus extract may be a potential anti-tumorigenic agent. Some research showed that the total saponins obtained from Astragalus membranaceus possess significant antitumorigenic activity. Gastric cancer is one of the most frequent cancers in the world, almost two-thirds of gastric cancer cases and deaths occur in less developed regions. But the effect of Astragalus membranaceus on proliferation, invasion and apoptosis of gastric cancer BGC-823 cells remains unclear.

Methods: Astragalus saponins were extracted. Cells proliferation was determined by CCK-8 assay. Cell cycle and apoptosis were detected by the flow cytometry. Boyden chamber was used to evaluate the invasion and metastasis capabilities of BGC-823 cells. Tumor growth was assessed by subcutaneous inoculation of cells into BALB/c nude mice.

Results: The results demonstrated that total Astragalus saponins could inhibit human gastric cancer cell growth both in vitro and in vivo, in additional, Astragalus saponins deceased the invasion ability and induced the apoptosis of gastric cancer BGC-823 cells.

Conclusions: Total Astragalus saponins inhibited human gastric cancer cell growth, decreased the invasion ability and induced the apoptosis. This suggested the possibility of further developing Astragalus as an alternative treatment option, or perhaps using it as adjuvant chemotherapeutic agent in gastric cancer therapy.

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Related in: MedlinePlus

Astragalus saponins decreased the invasion and migration ability of BGC-823 cells. A. BGC-823 cell invasion was determined using the transwell invasion assay, which measures the number of cells that migrate through the matrigel into the lower surface of the polycarbonic membrane (100×). Data are presented as the mean of triplicate experiments. B. Cell invasion decreased significantly (*p < 0.05) in a dose-dependent manner in Astragalus saponins -treated cells compared with the control group.
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Figure 2: Astragalus saponins decreased the invasion and migration ability of BGC-823 cells. A. BGC-823 cell invasion was determined using the transwell invasion assay, which measures the number of cells that migrate through the matrigel into the lower surface of the polycarbonic membrane (100×). Data are presented as the mean of triplicate experiments. B. Cell invasion decreased significantly (*p < 0.05) in a dose-dependent manner in Astragalus saponins -treated cells compared with the control group.

Mentions: Transwell invasion assay was used to evaluate the impact of Astragalus saponins on cell invasion and migration. BGC-823 cells were treated with 0 μg/ml, 20 μg/ml, 40 μg/ml, 80 μg/ml Astragalus saponins, and then placed in a Transwell chamber. The number of Astragalus saponins treated BGC-823 cells migrating through the matrigel was significantly lower (p < 0.05) than those of the 0 μg/ml Astragalus saponins group (the control group), (Figure 2). The effect was more obvious for 80 μg/ml Astragalus saponins. These results demonstrated that Astragalus saponins inhibited the invasive ability of BGC-823 cells in vitro.


Astragalus saponins affect proliferation, invasion and apoptosis of gastric cancer BGC-823 cells.

Wang T, Xuan X, Li M, Gao P, Zheng Y, Zang W, Zhao G - Diagn Pathol (2013)

Astragalus saponins decreased the invasion and migration ability of BGC-823 cells. A. BGC-823 cell invasion was determined using the transwell invasion assay, which measures the number of cells that migrate through the matrigel into the lower surface of the polycarbonic membrane (100×). Data are presented as the mean of triplicate experiments. B. Cell invasion decreased significantly (*p < 0.05) in a dose-dependent manner in Astragalus saponins -treated cells compared with the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3818446&req=5

Figure 2: Astragalus saponins decreased the invasion and migration ability of BGC-823 cells. A. BGC-823 cell invasion was determined using the transwell invasion assay, which measures the number of cells that migrate through the matrigel into the lower surface of the polycarbonic membrane (100×). Data are presented as the mean of triplicate experiments. B. Cell invasion decreased significantly (*p < 0.05) in a dose-dependent manner in Astragalus saponins -treated cells compared with the control group.
Mentions: Transwell invasion assay was used to evaluate the impact of Astragalus saponins on cell invasion and migration. BGC-823 cells were treated with 0 μg/ml, 20 μg/ml, 40 μg/ml, 80 μg/ml Astragalus saponins, and then placed in a Transwell chamber. The number of Astragalus saponins treated BGC-823 cells migrating through the matrigel was significantly lower (p < 0.05) than those of the 0 μg/ml Astragalus saponins group (the control group), (Figure 2). The effect was more obvious for 80 μg/ml Astragalus saponins. These results demonstrated that Astragalus saponins inhibited the invasive ability of BGC-823 cells in vitro.

Bottom Line: Cells proliferation was determined by CCK-8 assay.Cell cycle and apoptosis were detected by the flow cytometry.Total Astragalus saponins inhibited human gastric cancer cell growth, decreased the invasion ability and induced the apoptosis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China. zangwenqiao@sina.com.

ABSTRACT

Background: Astragalus memebranaceus is a traditional Chinese herbal medicine used in treatment of common cold, diarrhea, fatigue, anorexia and cardiac diseases. Recently, there are growing evidences that Astragalus extract may be a potential anti-tumorigenic agent. Some research showed that the total saponins obtained from Astragalus membranaceus possess significant antitumorigenic activity. Gastric cancer is one of the most frequent cancers in the world, almost two-thirds of gastric cancer cases and deaths occur in less developed regions. But the effect of Astragalus membranaceus on proliferation, invasion and apoptosis of gastric cancer BGC-823 cells remains unclear.

Methods: Astragalus saponins were extracted. Cells proliferation was determined by CCK-8 assay. Cell cycle and apoptosis were detected by the flow cytometry. Boyden chamber was used to evaluate the invasion and metastasis capabilities of BGC-823 cells. Tumor growth was assessed by subcutaneous inoculation of cells into BALB/c nude mice.

Results: The results demonstrated that total Astragalus saponins could inhibit human gastric cancer cell growth both in vitro and in vivo, in additional, Astragalus saponins deceased the invasion ability and induced the apoptosis of gastric cancer BGC-823 cells.

Conclusions: Total Astragalus saponins inhibited human gastric cancer cell growth, decreased the invasion ability and induced the apoptosis. This suggested the possibility of further developing Astragalus as an alternative treatment option, or perhaps using it as adjuvant chemotherapeutic agent in gastric cancer therapy.

Show MeSH
Related in: MedlinePlus