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Increased expression of α-methylacyl-coenzyme A racemase (AMACR; p504s) and p16 in distal hyperplastic polyps.

Dayi N, Baba HA, Schmid KW, Schmitz KJ - Diagn Pathol (2013)

Bottom Line: Hyperplastic polyps (HP) and sessile serrated adenomas (SSA) share morphological similarities.In context of recently published data this suggest distal HPs as potential precursor lesions of certain adenoma subtypes.However, at this point of time this finding remains speculative and needs to be confirmed by further studies.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Pathology and Neuropathology, University of Duisburg-Essen, Hufelandstrasse 55, 45122 Essen, Germany. kjschmitz@pathologie-re.de.

ABSTRACT

Background: Hyperplastic polyps (HP) and sessile serrated adenomas (SSA) share morphological similarities. In this immunohistochemical study we chose a panel of potential relevant and promising biomarkers including α-methylacyl-coenzyme A racemase (AMACR; p504s), which is involved in the degradation of branched chained fatty acids derivates, and analysed a cohort of HPs and SSAs in order to identify different immunophenotypes in relation to lesion localisation.

Methods: 154 specimen were carefully selected and a micro tissue array (TMA) was constructed. Immunohistochemistry of p16Ink4a, Ki67, α-methylacyl-coenzyme A racemase (AMACR; p504s), BRAF, CK 20, MLH1 and β-catenin was performed and and immunoexpression was compared among proximal and distal HPs as well as SSAs.

Results: None of the markers revealed a differential expression among HPs and SSAs. However, the study demonstrates a significant overexpression of AMACR (p = 0.004) and p16Ink4a (p = 0.028) in distal HPs compared to proximal HPs. In addition AMACR overexpression was associated with increased p16Ink4a immunoexpression (p < 0.001).

Conclusions: In this study we describe differential AMACR and p16Ink4a in HPs in relation to their localisation. Distal HPs were characterized by AMACR and p16Ink4a overexpression in contrast to proximal HPs, although morphological identically. Thus AMACR overexpression points towards a pathobiological relevance of the protein in distal HPs. In context of recently published data this suggest distal HPs as potential precursor lesions of certain adenoma subtypes. However, at this point of time this finding remains speculative and needs to be confirmed by further studies.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1836116001066768.

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On the left HP with strong basal AMACR immunoexpression. On the right higher magnification of cytoplasmic granular AMACR expression at the base of the typically L-shaped, dilated SSA crypts (magnification × 400).
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Figure 1: On the left HP with strong basal AMACR immunoexpression. On the right higher magnification of cytoplasmic granular AMACR expression at the base of the typically L-shaped, dilated SSA crypts (magnification × 400).

Mentions: AMACR immunostaining was located in the cytoplasm of upper and basal crypt cells. Only basal location was analysed. Regarding the group of HPs, χ2 analysis showed, that distal HPs exhibited statistically significantly positive AMACR expression (p < 0.004; Table 4). Figure 1 demonstrates AMACR expression in SSA and HP.


Increased expression of α-methylacyl-coenzyme A racemase (AMACR; p504s) and p16 in distal hyperplastic polyps.

Dayi N, Baba HA, Schmid KW, Schmitz KJ - Diagn Pathol (2013)

On the left HP with strong basal AMACR immunoexpression. On the right higher magnification of cytoplasmic granular AMACR expression at the base of the typically L-shaped, dilated SSA crypts (magnification × 400).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3818439&req=5

Figure 1: On the left HP with strong basal AMACR immunoexpression. On the right higher magnification of cytoplasmic granular AMACR expression at the base of the typically L-shaped, dilated SSA crypts (magnification × 400).
Mentions: AMACR immunostaining was located in the cytoplasm of upper and basal crypt cells. Only basal location was analysed. Regarding the group of HPs, χ2 analysis showed, that distal HPs exhibited statistically significantly positive AMACR expression (p < 0.004; Table 4). Figure 1 demonstrates AMACR expression in SSA and HP.

Bottom Line: Hyperplastic polyps (HP) and sessile serrated adenomas (SSA) share morphological similarities.In context of recently published data this suggest distal HPs as potential precursor lesions of certain adenoma subtypes.However, at this point of time this finding remains speculative and needs to be confirmed by further studies.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Pathology and Neuropathology, University of Duisburg-Essen, Hufelandstrasse 55, 45122 Essen, Germany. kjschmitz@pathologie-re.de.

ABSTRACT

Background: Hyperplastic polyps (HP) and sessile serrated adenomas (SSA) share morphological similarities. In this immunohistochemical study we chose a panel of potential relevant and promising biomarkers including α-methylacyl-coenzyme A racemase (AMACR; p504s), which is involved in the degradation of branched chained fatty acids derivates, and analysed a cohort of HPs and SSAs in order to identify different immunophenotypes in relation to lesion localisation.

Methods: 154 specimen were carefully selected and a micro tissue array (TMA) was constructed. Immunohistochemistry of p16Ink4a, Ki67, α-methylacyl-coenzyme A racemase (AMACR; p504s), BRAF, CK 20, MLH1 and β-catenin was performed and and immunoexpression was compared among proximal and distal HPs as well as SSAs.

Results: None of the markers revealed a differential expression among HPs and SSAs. However, the study demonstrates a significant overexpression of AMACR (p = 0.004) and p16Ink4a (p = 0.028) in distal HPs compared to proximal HPs. In addition AMACR overexpression was associated with increased p16Ink4a immunoexpression (p < 0.001).

Conclusions: In this study we describe differential AMACR and p16Ink4a in HPs in relation to their localisation. Distal HPs were characterized by AMACR and p16Ink4a overexpression in contrast to proximal HPs, although morphological identically. Thus AMACR overexpression points towards a pathobiological relevance of the protein in distal HPs. In context of recently published data this suggest distal HPs as potential precursor lesions of certain adenoma subtypes. However, at this point of time this finding remains speculative and needs to be confirmed by further studies.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1836116001066768.

Show MeSH
Related in: MedlinePlus