Limits...
Tissue-specific and cation/anion-specific DNA methylation variations occurred in C. virgata in response to salinity stress.

Gao X, Cao D, Liu J, Wang X, Geng S, Liu B, Shi D - PLoS ONE (2013)

Bottom Line: The results showed that the effects on DNA methylation variations of single salts were ranked as follows: Na2CO3> NaHCO3> Na2SO4> NaCl, and their mixed salts exerted tissue-specific effects on C. virgata seedlings.These findings suggested that mixed salts were not the simple combination of single salts.Furthermore, not only single salts but also mixed salts showed tissue-specific and cations/anions-specific DNA methylation variations.

View Article: PubMed Central - PubMed

Affiliation: Institutes of Genetics and Cytology, Northeast Normal University, Changchun, China.

ABSTRACT
Salinity is a widespread environmental problem limiting productivity and growth of plants. Halophytes which can adapt and resist certain salt stress have various mechanisms to defend the higher salinity and alkalinity, and epigenetic mechanisms especially DNA methylation may play important roles in plant adaptability and plasticity. In this study, we aimed to investigate the different influences of various single salts (NaCl, Na2SO4, NaHCO3, Na2CO3) and their mixed salts on halophyte Chloris. virgata from the DNA methylation prospective, and discover the underlying relationships between specific DNA methylation variations and specific cations/anions through the methylation-sensitive amplification polymorphism analysis. The results showed that the effects on DNA methylation variations of single salts were ranked as follows: Na2CO3> NaHCO3> Na2SO4> NaCl, and their mixed salts exerted tissue-specific effects on C. virgata seedlings. Eight types of DNA methylation variations were detected and defined in C. virgata according to the specific cations/anions existed in stressful solutions; in addition, mix-specific and higher pH-specific bands were the main type in leaves and roots independently. These findings suggested that mixed salts were not the simple combination of single salts. Furthermore, not only single salts but also mixed salts showed tissue-specific and cations/anions-specific DNA methylation variations.

Show MeSH
Effects of different salt stresses on cytosine methylation level in C.virgata.A: control, B: NaCl, C: Na2SO4, D: NaHCO3, E: Na2CO3, F: Mix. Data represent means ±S.E. of four replicates. Different lower-case letters represent significant difference among treatments at the 5% level, according to least significant difference (LSD) test.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3818329&req=5

pone-0078426-g001: Effects of different salt stresses on cytosine methylation level in C.virgata.A: control, B: NaCl, C: Na2SO4, D: NaHCO3, E: Na2CO3, F: Mix. Data represent means ±S.E. of four replicates. Different lower-case letters represent significant difference among treatments at the 5% level, according to least significant difference (LSD) test.

Mentions: Comparing with control set, all treatments (B-F sets) led to changes of DNA methylation levels in both leaves and roots. In C. virgata leaves, the total methylation levels remained largely unchanged in B set, but was decreased dramatically in C-F sets (p<0.05) (Figure 1a). More complicated influences were observed in C. virgata roots, the total methylation level was decreased in B set but increased in E set dramatically (p<0.05) (Figure 1b), while remained largely unchanged in the other sets. We noted that mixed salts treatment decreased the methylation level (p<0.05) in leaves but not in roots.


Tissue-specific and cation/anion-specific DNA methylation variations occurred in C. virgata in response to salinity stress.

Gao X, Cao D, Liu J, Wang X, Geng S, Liu B, Shi D - PLoS ONE (2013)

Effects of different salt stresses on cytosine methylation level in C.virgata.A: control, B: NaCl, C: Na2SO4, D: NaHCO3, E: Na2CO3, F: Mix. Data represent means ±S.E. of four replicates. Different lower-case letters represent significant difference among treatments at the 5% level, according to least significant difference (LSD) test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818329&req=5

pone-0078426-g001: Effects of different salt stresses on cytosine methylation level in C.virgata.A: control, B: NaCl, C: Na2SO4, D: NaHCO3, E: Na2CO3, F: Mix. Data represent means ±S.E. of four replicates. Different lower-case letters represent significant difference among treatments at the 5% level, according to least significant difference (LSD) test.
Mentions: Comparing with control set, all treatments (B-F sets) led to changes of DNA methylation levels in both leaves and roots. In C. virgata leaves, the total methylation levels remained largely unchanged in B set, but was decreased dramatically in C-F sets (p<0.05) (Figure 1a). More complicated influences were observed in C. virgata roots, the total methylation level was decreased in B set but increased in E set dramatically (p<0.05) (Figure 1b), while remained largely unchanged in the other sets. We noted that mixed salts treatment decreased the methylation level (p<0.05) in leaves but not in roots.

Bottom Line: The results showed that the effects on DNA methylation variations of single salts were ranked as follows: Na2CO3> NaHCO3> Na2SO4> NaCl, and their mixed salts exerted tissue-specific effects on C. virgata seedlings.These findings suggested that mixed salts were not the simple combination of single salts.Furthermore, not only single salts but also mixed salts showed tissue-specific and cations/anions-specific DNA methylation variations.

View Article: PubMed Central - PubMed

Affiliation: Institutes of Genetics and Cytology, Northeast Normal University, Changchun, China.

ABSTRACT
Salinity is a widespread environmental problem limiting productivity and growth of plants. Halophytes which can adapt and resist certain salt stress have various mechanisms to defend the higher salinity and alkalinity, and epigenetic mechanisms especially DNA methylation may play important roles in plant adaptability and plasticity. In this study, we aimed to investigate the different influences of various single salts (NaCl, Na2SO4, NaHCO3, Na2CO3) and their mixed salts on halophyte Chloris. virgata from the DNA methylation prospective, and discover the underlying relationships between specific DNA methylation variations and specific cations/anions through the methylation-sensitive amplification polymorphism analysis. The results showed that the effects on DNA methylation variations of single salts were ranked as follows: Na2CO3> NaHCO3> Na2SO4> NaCl, and their mixed salts exerted tissue-specific effects on C. virgata seedlings. Eight types of DNA methylation variations were detected and defined in C. virgata according to the specific cations/anions existed in stressful solutions; in addition, mix-specific and higher pH-specific bands were the main type in leaves and roots independently. These findings suggested that mixed salts were not the simple combination of single salts. Furthermore, not only single salts but also mixed salts showed tissue-specific and cations/anions-specific DNA methylation variations.

Show MeSH