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Prenatal exposure to fenugreek impairs sensorimotor development and the operation of spinal cord networks in mice.

Khalki L, Ba M'hamed S, Sokar Z, Bennis M, Vinay L, Bras H, Viemari JC - PLoS ONE (2013)

Bottom Line: Fenugreek is a medicinal plant whose seeds are widely used in traditional medicine, mainly for its laxative, galactagogue and antidiabetic effects.The present study was conducted to evaluate the effects of prenatal treatment of fenugreek seeds on the development of sensorimotor functions from birth to young adults.On the other hand, the cross-correlation coefficient in control mice was significantly more negative than in treated animals indicating that alternating patterns are deteriorated in FSAE-treated animals.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire Pharmacologie, Neurobiologie et Comportement, Centre National de la Recherche Scientifiques et Techniques (URAC 37), Cadi Ayyad Université, Marrakech, Maroc ; Institut de Neurosciences de la Timone, P3M Team, CNRS, Aix Marseille Université, Marseille, France.

ABSTRACT
Fenugreek is a medicinal plant whose seeds are widely used in traditional medicine, mainly for its laxative, galactagogue and antidiabetic effects. However, consumption of fenugreek seeds during pregnancy has been associated with a range of congenital malformations, including hydrocephalus, anencephaly and spina bifida in humans. The present study was conducted to evaluate the effects of prenatal treatment of fenugreek seeds on the development of sensorimotor functions from birth to young adults. Pregnant mice were treated by gavage with 1 g/kg/day of lyophilized fenugreek seeds aqueous extract (FSAE) or distilled water during the gestational period. Behavioral tests revealed in prenatally treated mice a significant delay in righting, cliff avoidance, negative geotaxis responses and the swimming development. In addition, extracellular recording of motor output in spinal cord isolated from neonatal mice showed that the frequency of spontaneous activity and fictive locomotion was reduced in FSAE-exposed mice. On the other hand, the cross-correlation coefficient in control mice was significantly more negative than in treated animals indicating that alternating patterns are deteriorated in FSAE-treated animals. At advanced age, prenatally treated mice displayed altered locomotor coordination in the rotarod test and also changes in static and dynamic parameters assessed by the CatWalk automated gait analysis system. We conclude that FSAE impairs sensorimotor and coordination functions not only in neonates but also in adult mice. Moreover, spinal neuronal networks are less excitable in prenatally FSAE-exposed mice suggesting that modifications within the central nervous system are responsible, at least in part, for the motor impairments.

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Fenugreek exposure reduced the frequency of spontaneous bursts.(A) Spontaneous bursts of neuronal activity were recorded from L2 lumbar ventral roots in control (upper trace) and treated (lower trace) pups. (B) The frequency of spontaneous bursts was significantly lower in treated compared with control mice at different ages (P0- P1 and P2-P3). *P < 0.05, **P < 0.01.
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pone-0080013-g003: Fenugreek exposure reduced the frequency of spontaneous bursts.(A) Spontaneous bursts of neuronal activity were recorded from L2 lumbar ventral roots in control (upper trace) and treated (lower trace) pups. (B) The frequency of spontaneous bursts was significantly lower in treated compared with control mice at different ages (P0- P1 and P2-P3). *P < 0.05, **P < 0.01.

Mentions: We then examined whether spontaneous activity in the spinal cord was altered in prenatally treated mice. In control mice, recordings from L2 ventral roots (Figure 3A) revealed bursts of spontaneous activity at a frequency of 2.88 ± 0.43 at P0-P1 and 1.9 ± 0.24 bursts/min at P2-P3, respectively. This frequency was significantly reduced in treated mice (1.23 ± 0.12, U = 5, P = 0.009 and 0.92 ± 0.26, U = 3, P = 0.04 at P0-P1 and P2-P3, respectively; Figure 3B, 3C).


Prenatal exposure to fenugreek impairs sensorimotor development and the operation of spinal cord networks in mice.

Khalki L, Ba M'hamed S, Sokar Z, Bennis M, Vinay L, Bras H, Viemari JC - PLoS ONE (2013)

Fenugreek exposure reduced the frequency of spontaneous bursts.(A) Spontaneous bursts of neuronal activity were recorded from L2 lumbar ventral roots in control (upper trace) and treated (lower trace) pups. (B) The frequency of spontaneous bursts was significantly lower in treated compared with control mice at different ages (P0- P1 and P2-P3). *P < 0.05, **P < 0.01.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818278&req=5

pone-0080013-g003: Fenugreek exposure reduced the frequency of spontaneous bursts.(A) Spontaneous bursts of neuronal activity were recorded from L2 lumbar ventral roots in control (upper trace) and treated (lower trace) pups. (B) The frequency of spontaneous bursts was significantly lower in treated compared with control mice at different ages (P0- P1 and P2-P3). *P < 0.05, **P < 0.01.
Mentions: We then examined whether spontaneous activity in the spinal cord was altered in prenatally treated mice. In control mice, recordings from L2 ventral roots (Figure 3A) revealed bursts of spontaneous activity at a frequency of 2.88 ± 0.43 at P0-P1 and 1.9 ± 0.24 bursts/min at P2-P3, respectively. This frequency was significantly reduced in treated mice (1.23 ± 0.12, U = 5, P = 0.009 and 0.92 ± 0.26, U = 3, P = 0.04 at P0-P1 and P2-P3, respectively; Figure 3B, 3C).

Bottom Line: Fenugreek is a medicinal plant whose seeds are widely used in traditional medicine, mainly for its laxative, galactagogue and antidiabetic effects.The present study was conducted to evaluate the effects of prenatal treatment of fenugreek seeds on the development of sensorimotor functions from birth to young adults.On the other hand, the cross-correlation coefficient in control mice was significantly more negative than in treated animals indicating that alternating patterns are deteriorated in FSAE-treated animals.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire Pharmacologie, Neurobiologie et Comportement, Centre National de la Recherche Scientifiques et Techniques (URAC 37), Cadi Ayyad Université, Marrakech, Maroc ; Institut de Neurosciences de la Timone, P3M Team, CNRS, Aix Marseille Université, Marseille, France.

ABSTRACT
Fenugreek is a medicinal plant whose seeds are widely used in traditional medicine, mainly for its laxative, galactagogue and antidiabetic effects. However, consumption of fenugreek seeds during pregnancy has been associated with a range of congenital malformations, including hydrocephalus, anencephaly and spina bifida in humans. The present study was conducted to evaluate the effects of prenatal treatment of fenugreek seeds on the development of sensorimotor functions from birth to young adults. Pregnant mice were treated by gavage with 1 g/kg/day of lyophilized fenugreek seeds aqueous extract (FSAE) or distilled water during the gestational period. Behavioral tests revealed in prenatally treated mice a significant delay in righting, cliff avoidance, negative geotaxis responses and the swimming development. In addition, extracellular recording of motor output in spinal cord isolated from neonatal mice showed that the frequency of spontaneous activity and fictive locomotion was reduced in FSAE-exposed mice. On the other hand, the cross-correlation coefficient in control mice was significantly more negative than in treated animals indicating that alternating patterns are deteriorated in FSAE-treated animals. At advanced age, prenatally treated mice displayed altered locomotor coordination in the rotarod test and also changes in static and dynamic parameters assessed by the CatWalk automated gait analysis system. We conclude that FSAE impairs sensorimotor and coordination functions not only in neonates but also in adult mice. Moreover, spinal neuronal networks are less excitable in prenatally FSAE-exposed mice suggesting that modifications within the central nervous system are responsible, at least in part, for the motor impairments.

Show MeSH
Related in: MedlinePlus