Limits...
SERPINE1, PAI-1 protein coding gene, methylation levels and epigenetic relationships with adiposity changes in obese subjects with metabolic syndrome features under dietary restriction.

Lopez-Legarrea P, Mansego ML, Zulet MA, Martinez JA - J Clin Biochem Nutr (2013)

Bottom Line: Both dietary strategies reduced anthropometric and biochemical parameters.The Control group significantly reduced plasma PAI-1 concentrations but not the RESMENA group.Participants from both nutritional interventions with higher SERPINE1 methylation levels at baseline showed significantly major reductions in body weight, total fat mass, android fat mass, total cholesterol and triglycerides, as compared with those with lower initial SERPINE1 methylation levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition, Food Science and Physiology, University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain.

ABSTRACT
Plasminogen activator inhibitor 1 (PAI-1) has been associated with metabolic disorders, through different mechanisms, which could involve changes in DNA methylation. This work aimed to assess the potential relationships of the cytosine methylation levels within SERPINE1 gene transcriptional regulatory region, which codes for PAI-1, in peripheral white blood cells with anthropometrical, metabolic and inflammatory features. Forty-six obese subjects with metabolic syndrome features followed Control or Metabolic Syndrome Reduction in Navarra (RESMENA) energy-restricted (-30%E) diets for 8 weeks. SERPINE1 transcriptional regulatory region methylation at baseline was analyzed by a microarray technical. Both dietary strategies reduced anthropometric and biochemical parameters. The Control group significantly reduced plasma PAI-1 concentrations but not the RESMENA group. Participants from both nutritional interventions with higher SERPINE1 methylation levels at baseline showed significantly major reductions in body weight, total fat mass, android fat mass, total cholesterol and triglycerides, as compared with those with lower initial SERPINE1 methylation levels. In conclusion, the DNA methylation levels of SERPINE1 transcriptional regulatory region were associated with some metabolic and anthropometric changes in obese subjects with metabolic syndrome under energy restriction, suggesting a complex epigenetic network in the regulation of this recognized pro-inflammatory marker. (www.clinicaltrials.gov; NCT01087086).

No MeSH data available.


Related in: MedlinePlus

Changes in PAI-1 plasma concentrations in Control and RESMENA groups from                    baseline to the endpoint of the intervention and differences between them (A);                    comparison between Control and RESMENA groups levels of                        SERPINE1 CpG 10 methylation (%) at baseline (B); comparison                    of PAI-1 plasma concentrations within Control and RESMENA groups according to                        SERPINE1 CpG 10 baseline methylation level (%) (C) and                    correlation analysis between methylation in the SERPINE1 CpG 10                    and total fat mass by Dual-energy X-ray absorptiometry at baseline (D). PAI-1:                    plasminogen activator inhibitor 1.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3818272&req=5

Figure 2: Changes in PAI-1 plasma concentrations in Control and RESMENA groups from baseline to the endpoint of the intervention and differences between them (A); comparison between Control and RESMENA groups levels of SERPINE1 CpG 10 methylation (%) at baseline (B); comparison of PAI-1 plasma concentrations within Control and RESMENA groups according to SERPINE1 CpG 10 baseline methylation level (%) (C) and correlation analysis between methylation in the SERPINE1 CpG 10 and total fat mass by Dual-energy X-ray absorptiometry at baseline (D). PAI-1: plasminogen activator inhibitor 1.

Mentions: Statistical between-diet differences were not found (p>0.05) after the 8 weeks of dietary intervention, neither for anthropometric, nor for routine biochemical parameters (Table 2). PAI-1 concentration levels were significantly decreased within the Control group (p = 0.008) whereas this marker did not show statistical variations within the RESMENA group (Fig. 2A). There were not between-group differences concerning SERPINE1 methylation levels at baseline (p = 0.260, Fig. 2B). We did not find any interaction between the dietary group and SERPINE1 methylation levels (Fig. 2C). Therefore, the sample was merged and considered as a whole for subsequent analyses. Interestingly, a preliminary analysis showed significant associations between baseline SERPINE1 methylation levels and the changes in body weight (r = –0.360, p = 0.016), total fat mass (r = –0.354, p = 0.018) and android fat mass (r = –0.330, p = 0.029), which were not found for PAI-1 plasma concentrations. For further assessing the relationships between changes on anthropometrical variables, clinical and metabolic features, energy intake as well as pro-inflammatory markers concentrations and SERPINE1 methylation, the sample was categorized according to median DNA methylation baseline levels of SERPINE1 transcriptional regulatory region (Table 3). Differences were found neither on BMI, nor on waist circumference due to methylation level. However body weight and body composition-related variables changes were statistically different according to SERPINE1 transcriptional regulatory region methylation. In addition, total fat mass significantly correlated with DNA methylation levels of SERPINE1 CpG 10 site (Fig. 2D).


SERPINE1, PAI-1 protein coding gene, methylation levels and epigenetic relationships with adiposity changes in obese subjects with metabolic syndrome features under dietary restriction.

Lopez-Legarrea P, Mansego ML, Zulet MA, Martinez JA - J Clin Biochem Nutr (2013)

Changes in PAI-1 plasma concentrations in Control and RESMENA groups from                    baseline to the endpoint of the intervention and differences between them (A);                    comparison between Control and RESMENA groups levels of                        SERPINE1 CpG 10 methylation (%) at baseline (B); comparison                    of PAI-1 plasma concentrations within Control and RESMENA groups according to                        SERPINE1 CpG 10 baseline methylation level (%) (C) and                    correlation analysis between methylation in the SERPINE1 CpG 10                    and total fat mass by Dual-energy X-ray absorptiometry at baseline (D). PAI-1:                    plasminogen activator inhibitor 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818272&req=5

Figure 2: Changes in PAI-1 plasma concentrations in Control and RESMENA groups from baseline to the endpoint of the intervention and differences between them (A); comparison between Control and RESMENA groups levels of SERPINE1 CpG 10 methylation (%) at baseline (B); comparison of PAI-1 plasma concentrations within Control and RESMENA groups according to SERPINE1 CpG 10 baseline methylation level (%) (C) and correlation analysis between methylation in the SERPINE1 CpG 10 and total fat mass by Dual-energy X-ray absorptiometry at baseline (D). PAI-1: plasminogen activator inhibitor 1.
Mentions: Statistical between-diet differences were not found (p>0.05) after the 8 weeks of dietary intervention, neither for anthropometric, nor for routine biochemical parameters (Table 2). PAI-1 concentration levels were significantly decreased within the Control group (p = 0.008) whereas this marker did not show statistical variations within the RESMENA group (Fig. 2A). There were not between-group differences concerning SERPINE1 methylation levels at baseline (p = 0.260, Fig. 2B). We did not find any interaction between the dietary group and SERPINE1 methylation levels (Fig. 2C). Therefore, the sample was merged and considered as a whole for subsequent analyses. Interestingly, a preliminary analysis showed significant associations between baseline SERPINE1 methylation levels and the changes in body weight (r = –0.360, p = 0.016), total fat mass (r = –0.354, p = 0.018) and android fat mass (r = –0.330, p = 0.029), which were not found for PAI-1 plasma concentrations. For further assessing the relationships between changes on anthropometrical variables, clinical and metabolic features, energy intake as well as pro-inflammatory markers concentrations and SERPINE1 methylation, the sample was categorized according to median DNA methylation baseline levels of SERPINE1 transcriptional regulatory region (Table 3). Differences were found neither on BMI, nor on waist circumference due to methylation level. However body weight and body composition-related variables changes were statistically different according to SERPINE1 transcriptional regulatory region methylation. In addition, total fat mass significantly correlated with DNA methylation levels of SERPINE1 CpG 10 site (Fig. 2D).

Bottom Line: Both dietary strategies reduced anthropometric and biochemical parameters.The Control group significantly reduced plasma PAI-1 concentrations but not the RESMENA group.Participants from both nutritional interventions with higher SERPINE1 methylation levels at baseline showed significantly major reductions in body weight, total fat mass, android fat mass, total cholesterol and triglycerides, as compared with those with lower initial SERPINE1 methylation levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition, Food Science and Physiology, University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain.

ABSTRACT
Plasminogen activator inhibitor 1 (PAI-1) has been associated with metabolic disorders, through different mechanisms, which could involve changes in DNA methylation. This work aimed to assess the potential relationships of the cytosine methylation levels within SERPINE1 gene transcriptional regulatory region, which codes for PAI-1, in peripheral white blood cells with anthropometrical, metabolic and inflammatory features. Forty-six obese subjects with metabolic syndrome features followed Control or Metabolic Syndrome Reduction in Navarra (RESMENA) energy-restricted (-30%E) diets for 8 weeks. SERPINE1 transcriptional regulatory region methylation at baseline was analyzed by a microarray technical. Both dietary strategies reduced anthropometric and biochemical parameters. The Control group significantly reduced plasma PAI-1 concentrations but not the RESMENA group. Participants from both nutritional interventions with higher SERPINE1 methylation levels at baseline showed significantly major reductions in body weight, total fat mass, android fat mass, total cholesterol and triglycerides, as compared with those with lower initial SERPINE1 methylation levels. In conclusion, the DNA methylation levels of SERPINE1 transcriptional regulatory region were associated with some metabolic and anthropometric changes in obese subjects with metabolic syndrome under energy restriction, suggesting a complex epigenetic network in the regulation of this recognized pro-inflammatory marker. (www.clinicaltrials.gov; NCT01087086).

No MeSH data available.


Related in: MedlinePlus