Limits...
Induction of the hepatic stearoyl-CoA desaturase 1 gene in offspring after isocaloric administration of high fat sucrose diet during gestation.

Wanjihia VW, Ohminami H, Taketani Y, Amo K, Yamanaka-Okumura H, Yamamoto H, Takeda E - J Clin Biochem Nutr (2013)

Bottom Line: Adverse early nutrition leads to metabolic aberrations in adulthood.Molecular and cellular mechanisms responsible are emerging; specific nutritional causes remain unclarified.Gestational high sugar and fat intake even without over energy intake would be more detrimental to metabolisms of offspring compared to calorie restriction.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.

ABSTRACT
Adverse early nutrition leads to metabolic aberrations in adulthood. Molecular and cellular mechanisms responsible are emerging; specific nutritional causes remain unclarified. We investigated gestational dietary intake and its influences on metabolism in offspring. Three groups of pregnant Sprague-Dawley rats were fed either AIN93G standard diet as control, isocaloric high fat sucrose diet or calorie restriction diet (50% of control) until delivery. All dams were fed control diet ad libitum during lactation. Offsprings' metabolic parameters were assessed at three weeks. Visceral fat and plasma triglycerides of high fat sucrose diet offspring were significantly higher than those of control diet and calorie restriction diet offspring. Plasma leptin level was higher in high fat sucrose diet than control offspring. Conversely, plasma adiponectin was lower in high fat sucrose diet and calorie restriction diet offspring compared to controls. Significant inductions of hepatic mRNA expression of stearoyl-CoA desaturase1 and Δ-5 desaturase genes, were observed in high fat sucrose diet and calorie restriction diet offspring. Gestational high sugar and fat intake even without over energy intake would be more detrimental to metabolisms of offspring compared to calorie restriction.

No MeSH data available.


Body weight changes and oral glucose tolerance test in offspring. (A) Body weight of offspring was measured at birth and at 3 week-old. Data are mean ± SEM *p<0.05 vs CT group. (B) Oral glucose tolerance test in offspring was performed as described in Materials and Methods. After fasting for 13 h, the rats were given an oral dose of glucose 2 g/kg body weight. Blood samples were taken at 0, 30, 60, 90 and 120 min after glucose administration. Data are mean ± SEM, n = 6–10 rats/group, *p<0.01 vs CT group for CR at 60, 90 and 120 min and †p<0.05 vs CT group for HFS at 60, 90 and 120 min.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3818269&req=5

Figure 2: Body weight changes and oral glucose tolerance test in offspring. (A) Body weight of offspring was measured at birth and at 3 week-old. Data are mean ± SEM *p<0.05 vs CT group. (B) Oral glucose tolerance test in offspring was performed as described in Materials and Methods. After fasting for 13 h, the rats were given an oral dose of glucose 2 g/kg body weight. Blood samples were taken at 0, 30, 60, 90 and 120 min after glucose administration. Data are mean ± SEM, n = 6–10 rats/group, *p<0.01 vs CT group for CR at 60, 90 and 120 min and †p<0.05 vs CT group for HFS at 60, 90 and 120 min.

Mentions: The daily food intake and energy intake of CR diet group was just a half of control diet group (Fig. 1B and C). Conversely, the food intake of HFS diet group was slight but not significantly lower than that of control diet group (Fig. 1C). Resulting from the decrease in food intake in HFS diet group, the calculated daily energy intake of HFS diet group was same as that of control group (Fig. 1B). Therefore, this condition was defined as isocaloric administration of HFS diet (isocaloric HFS diet). The change in body weight among the dams was significantly lower in CR group compared to control group (Fig. 1A). Gestational calorie restriction caused intra-uterine growth retardation (IUGR) in offspring of the CR diet group and their body weight at 3 weeks remained significantly lower as opposed to the isocaloric HFS diet and control diet groups (Table 3 and Fig. 2A).


Induction of the hepatic stearoyl-CoA desaturase 1 gene in offspring after isocaloric administration of high fat sucrose diet during gestation.

Wanjihia VW, Ohminami H, Taketani Y, Amo K, Yamanaka-Okumura H, Yamamoto H, Takeda E - J Clin Biochem Nutr (2013)

Body weight changes and oral glucose tolerance test in offspring. (A) Body weight of offspring was measured at birth and at 3 week-old. Data are mean ± SEM *p<0.05 vs CT group. (B) Oral glucose tolerance test in offspring was performed as described in Materials and Methods. After fasting for 13 h, the rats were given an oral dose of glucose 2 g/kg body weight. Blood samples were taken at 0, 30, 60, 90 and 120 min after glucose administration. Data are mean ± SEM, n = 6–10 rats/group, *p<0.01 vs CT group for CR at 60, 90 and 120 min and †p<0.05 vs CT group for HFS at 60, 90 and 120 min.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818269&req=5

Figure 2: Body weight changes and oral glucose tolerance test in offspring. (A) Body weight of offspring was measured at birth and at 3 week-old. Data are mean ± SEM *p<0.05 vs CT group. (B) Oral glucose tolerance test in offspring was performed as described in Materials and Methods. After fasting for 13 h, the rats were given an oral dose of glucose 2 g/kg body weight. Blood samples were taken at 0, 30, 60, 90 and 120 min after glucose administration. Data are mean ± SEM, n = 6–10 rats/group, *p<0.01 vs CT group for CR at 60, 90 and 120 min and †p<0.05 vs CT group for HFS at 60, 90 and 120 min.
Mentions: The daily food intake and energy intake of CR diet group was just a half of control diet group (Fig. 1B and C). Conversely, the food intake of HFS diet group was slight but not significantly lower than that of control diet group (Fig. 1C). Resulting from the decrease in food intake in HFS diet group, the calculated daily energy intake of HFS diet group was same as that of control group (Fig. 1B). Therefore, this condition was defined as isocaloric administration of HFS diet (isocaloric HFS diet). The change in body weight among the dams was significantly lower in CR group compared to control group (Fig. 1A). Gestational calorie restriction caused intra-uterine growth retardation (IUGR) in offspring of the CR diet group and their body weight at 3 weeks remained significantly lower as opposed to the isocaloric HFS diet and control diet groups (Table 3 and Fig. 2A).

Bottom Line: Adverse early nutrition leads to metabolic aberrations in adulthood.Molecular and cellular mechanisms responsible are emerging; specific nutritional causes remain unclarified.Gestational high sugar and fat intake even without over energy intake would be more detrimental to metabolisms of offspring compared to calorie restriction.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.

ABSTRACT
Adverse early nutrition leads to metabolic aberrations in adulthood. Molecular and cellular mechanisms responsible are emerging; specific nutritional causes remain unclarified. We investigated gestational dietary intake and its influences on metabolism in offspring. Three groups of pregnant Sprague-Dawley rats were fed either AIN93G standard diet as control, isocaloric high fat sucrose diet or calorie restriction diet (50% of control) until delivery. All dams were fed control diet ad libitum during lactation. Offsprings' metabolic parameters were assessed at three weeks. Visceral fat and plasma triglycerides of high fat sucrose diet offspring were significantly higher than those of control diet and calorie restriction diet offspring. Plasma leptin level was higher in high fat sucrose diet than control offspring. Conversely, plasma adiponectin was lower in high fat sucrose diet and calorie restriction diet offspring compared to controls. Significant inductions of hepatic mRNA expression of stearoyl-CoA desaturase1 and Δ-5 desaturase genes, were observed in high fat sucrose diet and calorie restriction diet offspring. Gestational high sugar and fat intake even without over energy intake would be more detrimental to metabolisms of offspring compared to calorie restriction.

No MeSH data available.