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Evaluation of biochemical effects of Casuarina equisetifolia extract on gentamicin-induced nephrotoxicity and oxidative stress in rats. Phytochemical analysis.

El-Tantawy WH, Mohamed SA, Abd Al Haleem EN - J Clin Biochem Nutr (2013)

Bottom Line: Also oxidative stress was noticed in renal tissue as evidenced by a significant decrease in glutathione level, superoxide dismutase, glutathione-S-transferase activities, also a significant increase in malondialdehyde and nitric oxide levels when compared to control group.Administration of plant extract at a dose of 300 mg/kg once daily for 4 weeks restored normal renal functions and attenuated oxidative stress.In conclusion, Casuarina equisetifolia leaves extract ameliorates gentamicin-induced nephrotoxicity and oxidative damage by scavenging oxygen free radicals, decreasing lipid peroxidation and improving intracellular antioxidant defense, thus extract may be used as nephroprotective agent.

View Article: PubMed Central - PubMed

Affiliation: National Organization For Drug Control & Research, P.O.29 Cairo, Egypt.

ABSTRACT
Nephrotoxicity is defined as renal dysfunction that arises as result of exposure to external agents such as drugs and environmental chemicals. The present work was undertaken to carry out the phytochemical study and nephroprotective activity of methanolic extract of Casuarina equisetifolia leaves in gentamicin-induced nephrotoxicity in Wistar rats. Flavonoids and phenolic acids were identified and quantified using high performance liquid chromatography. Subcutaneous injection of rats with gentamicin (80 mg/kg body weight/day) for six consecutive days induced marked acute renal toxicity, manifested by a significant increase in serum urea, creatinine and uric acid levels, along with a significant depletion of serum potassium level, compared to normal controls. Also oxidative stress was noticed in renal tissue as evidenced by a significant decrease in glutathione level, superoxide dismutase, glutathione-S-transferase activities, also a significant increase in malondialdehyde and nitric oxide levels when compared to control group. Administration of plant extract at a dose of 300 mg/kg once daily for 4 weeks restored normal renal functions and attenuated oxidative stress. In conclusion, Casuarina equisetifolia leaves extract ameliorates gentamicin-induced nephrotoxicity and oxidative damage by scavenging oxygen free radicals, decreasing lipid peroxidation and improving intracellular antioxidant defense, thus extract may be used as nephroprotective agent.

No MeSH data available.


Related in: MedlinePlus

HPLC chromatograms of identified phenolic compounds of Casuarina equisetifolia leaves extract separated by RP HPLC; Pyrogallol (Rt = 6.7 min), Gallic (Rt = 7 min), Protocatechuic (Rt = 8.2 min), Chlorogenic (Rt = 8.7 min), Catechol (Rt = 8.9 min), Salicylic (Rt = 11.3 min), P-Coumaric (Rt = 12.79 min), Benzoic (Rt = 13.54 min), Chrysin (Rt = 18.2 min).
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Figure 1: HPLC chromatograms of identified phenolic compounds of Casuarina equisetifolia leaves extract separated by RP HPLC; Pyrogallol (Rt = 6.7 min), Gallic (Rt = 7 min), Protocatechuic (Rt = 8.2 min), Chlorogenic (Rt = 8.7 min), Catechol (Rt = 8.9 min), Salicylic (Rt = 11.3 min), P-Coumaric (Rt = 12.79 min), Benzoic (Rt = 13.54 min), Chrysin (Rt = 18.2 min).

Mentions: Fig. 1 represents the separation of phenolic compounds of Casuarina equisetifolia leaves extract. HPLC analysis of the total phenolic contents of methanolic extract of the aerial parts of Casuarina equisetifolia revealed the presence of 9 phenolic compounds as gallic acid, protochatechuic acid, para-coumaric acid, chlorogenic acid, salicylic acid, benzoic acid, catechol, pyrogallol and chrysin in concentration of (21.41, 13.15, 0.73, 129.42, 3.14, 181.39, 16.17, 1,049.9 and 3.04 mg/100 g plant extract respectively). Pyrogallol represented the highest concentration of the identified phenolic compounds while para-coumaric acid was the lowest concentration (Table 2).


Evaluation of biochemical effects of Casuarina equisetifolia extract on gentamicin-induced nephrotoxicity and oxidative stress in rats. Phytochemical analysis.

El-Tantawy WH, Mohamed SA, Abd Al Haleem EN - J Clin Biochem Nutr (2013)

HPLC chromatograms of identified phenolic compounds of Casuarina equisetifolia leaves extract separated by RP HPLC; Pyrogallol (Rt = 6.7 min), Gallic (Rt = 7 min), Protocatechuic (Rt = 8.2 min), Chlorogenic (Rt = 8.7 min), Catechol (Rt = 8.9 min), Salicylic (Rt = 11.3 min), P-Coumaric (Rt = 12.79 min), Benzoic (Rt = 13.54 min), Chrysin (Rt = 18.2 min).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818266&req=5

Figure 1: HPLC chromatograms of identified phenolic compounds of Casuarina equisetifolia leaves extract separated by RP HPLC; Pyrogallol (Rt = 6.7 min), Gallic (Rt = 7 min), Protocatechuic (Rt = 8.2 min), Chlorogenic (Rt = 8.7 min), Catechol (Rt = 8.9 min), Salicylic (Rt = 11.3 min), P-Coumaric (Rt = 12.79 min), Benzoic (Rt = 13.54 min), Chrysin (Rt = 18.2 min).
Mentions: Fig. 1 represents the separation of phenolic compounds of Casuarina equisetifolia leaves extract. HPLC analysis of the total phenolic contents of methanolic extract of the aerial parts of Casuarina equisetifolia revealed the presence of 9 phenolic compounds as gallic acid, protochatechuic acid, para-coumaric acid, chlorogenic acid, salicylic acid, benzoic acid, catechol, pyrogallol and chrysin in concentration of (21.41, 13.15, 0.73, 129.42, 3.14, 181.39, 16.17, 1,049.9 and 3.04 mg/100 g plant extract respectively). Pyrogallol represented the highest concentration of the identified phenolic compounds while para-coumaric acid was the lowest concentration (Table 2).

Bottom Line: Also oxidative stress was noticed in renal tissue as evidenced by a significant decrease in glutathione level, superoxide dismutase, glutathione-S-transferase activities, also a significant increase in malondialdehyde and nitric oxide levels when compared to control group.Administration of plant extract at a dose of 300 mg/kg once daily for 4 weeks restored normal renal functions and attenuated oxidative stress.In conclusion, Casuarina equisetifolia leaves extract ameliorates gentamicin-induced nephrotoxicity and oxidative damage by scavenging oxygen free radicals, decreasing lipid peroxidation and improving intracellular antioxidant defense, thus extract may be used as nephroprotective agent.

View Article: PubMed Central - PubMed

Affiliation: National Organization For Drug Control & Research, P.O.29 Cairo, Egypt.

ABSTRACT
Nephrotoxicity is defined as renal dysfunction that arises as result of exposure to external agents such as drugs and environmental chemicals. The present work was undertaken to carry out the phytochemical study and nephroprotective activity of methanolic extract of Casuarina equisetifolia leaves in gentamicin-induced nephrotoxicity in Wistar rats. Flavonoids and phenolic acids were identified and quantified using high performance liquid chromatography. Subcutaneous injection of rats with gentamicin (80 mg/kg body weight/day) for six consecutive days induced marked acute renal toxicity, manifested by a significant increase in serum urea, creatinine and uric acid levels, along with a significant depletion of serum potassium level, compared to normal controls. Also oxidative stress was noticed in renal tissue as evidenced by a significant decrease in glutathione level, superoxide dismutase, glutathione-S-transferase activities, also a significant increase in malondialdehyde and nitric oxide levels when compared to control group. Administration of plant extract at a dose of 300 mg/kg once daily for 4 weeks restored normal renal functions and attenuated oxidative stress. In conclusion, Casuarina equisetifolia leaves extract ameliorates gentamicin-induced nephrotoxicity and oxidative damage by scavenging oxygen free radicals, decreasing lipid peroxidation and improving intracellular antioxidant defense, thus extract may be used as nephroprotective agent.

No MeSH data available.


Related in: MedlinePlus