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Changes in brain tissue and behavior patterns induced by single short-term fasting in mice.

Hisatomi Y, Asakura K, Kugino K, Kurokawa M, Asakura T, Nakata K - PLoS ONE (2013)

Bottom Line: However, the effects of single short-term fasting on brain tissue or behavioral activity patterns remain unclear.The brain weight did not change by a fast, and brain atrophy was not observed.However, it is interesting that the results of this study suggest that single short-term fasting has an effect on encephalic neuropoiesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Health and Nutrition Science, Faculty of Health and Social Welfare Science, Nishikyushu University, Kanzaki, Saga, Japan.

ABSTRACT
In humans, emaciation from long-term dietary deficiencies, such as anorexia, reportedly increases physical activity and brain atrophy. However, the effects of single short-term fasting on brain tissue or behavioral activity patterns remain unclear. To clarify the impact of malnutrition on brain function, we conducted a single short-term fasting study as an anorexia model using male adult mice and determined if changes occurred in migratory behavior as an expression of brain function and in brain tissue structure. Sixteen-week-old C57BL/6J male mice were divided into either the fasted group or the control group. Experiments were conducted in a fixed indoor environment. We examined the effects of fasting on the number of nerve cells, structural changes in the myelin and axon density, and brain atrophy. For behavior observation, the amount of food and water consumed, ingestion time, and the pattern of movement were measured using a time-recording system. The fasted mice showed a significant increase in physical activity and their rhythm of movement was disturbed. Since the brain was in an abnormal state after fasting, mice that were normally active during the night became active regardless of day or night and performed strenuous exercise at a high frequency. The brain weight did not change by a fast, and brain atrophy was not observed. Although no textural change was apparent by fasting, the neuronal neogenesis in the subventricular zone and hippocampus was inhibited, causing disorder of the brain function. A clear association between the suppression of encephalic neuropoiesis and overactivity was not established. However, it is interesting that the results of this study suggest that single short-term fasting has an effect on encephalic neuropoiesis.

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K2CABIN system and KUROBOX system.(A) K2CABIN system used to measure eating patterns and KUROBOX system used to measure movement patterns of behavior. (B),(C) KUROBOX; This measures the movement of mice by infrared sensors. We calculated the distance travelled by the mouse, the speed and the movement angle over time.
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pone-0080085-g002: K2CABIN system and KUROBOX system.(A) K2CABIN system used to measure eating patterns and KUROBOX system used to measure movement patterns of behavior. (B),(C) KUROBOX; This measures the movement of mice by infrared sensors. We calculated the distance travelled by the mouse, the speed and the movement angle over time.

Mentions: The mice were given CE-2 (CLEA Rodent Diet CE-2 for breeding) standard pellet chow. Tap water was used for drinking and was given ad libitum. Before the start of the experiment, mice were fed powder chow during a week-long acclimatization period in order to measure the feed intake in normal mice. In the first period, we measured food and water intake, and intake time using K2CABIN (Figure 2). In the second period, we measured the amount and rhythm of movement behavior using KUROBOX (Figure 2). In the third period, mice were fasted for 3 days, only drinking water was given ad libitum.


Changes in brain tissue and behavior patterns induced by single short-term fasting in mice.

Hisatomi Y, Asakura K, Kugino K, Kurokawa M, Asakura T, Nakata K - PLoS ONE (2013)

K2CABIN system and KUROBOX system.(A) K2CABIN system used to measure eating patterns and KUROBOX system used to measure movement patterns of behavior. (B),(C) KUROBOX; This measures the movement of mice by infrared sensors. We calculated the distance travelled by the mouse, the speed and the movement angle over time.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818263&req=5

pone-0080085-g002: K2CABIN system and KUROBOX system.(A) K2CABIN system used to measure eating patterns and KUROBOX system used to measure movement patterns of behavior. (B),(C) KUROBOX; This measures the movement of mice by infrared sensors. We calculated the distance travelled by the mouse, the speed and the movement angle over time.
Mentions: The mice were given CE-2 (CLEA Rodent Diet CE-2 for breeding) standard pellet chow. Tap water was used for drinking and was given ad libitum. Before the start of the experiment, mice were fed powder chow during a week-long acclimatization period in order to measure the feed intake in normal mice. In the first period, we measured food and water intake, and intake time using K2CABIN (Figure 2). In the second period, we measured the amount and rhythm of movement behavior using KUROBOX (Figure 2). In the third period, mice were fasted for 3 days, only drinking water was given ad libitum.

Bottom Line: However, the effects of single short-term fasting on brain tissue or behavioral activity patterns remain unclear.The brain weight did not change by a fast, and brain atrophy was not observed.However, it is interesting that the results of this study suggest that single short-term fasting has an effect on encephalic neuropoiesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Health and Nutrition Science, Faculty of Health and Social Welfare Science, Nishikyushu University, Kanzaki, Saga, Japan.

ABSTRACT
In humans, emaciation from long-term dietary deficiencies, such as anorexia, reportedly increases physical activity and brain atrophy. However, the effects of single short-term fasting on brain tissue or behavioral activity patterns remain unclear. To clarify the impact of malnutrition on brain function, we conducted a single short-term fasting study as an anorexia model using male adult mice and determined if changes occurred in migratory behavior as an expression of brain function and in brain tissue structure. Sixteen-week-old C57BL/6J male mice were divided into either the fasted group or the control group. Experiments were conducted in a fixed indoor environment. We examined the effects of fasting on the number of nerve cells, structural changes in the myelin and axon density, and brain atrophy. For behavior observation, the amount of food and water consumed, ingestion time, and the pattern of movement were measured using a time-recording system. The fasted mice showed a significant increase in physical activity and their rhythm of movement was disturbed. Since the brain was in an abnormal state after fasting, mice that were normally active during the night became active regardless of day or night and performed strenuous exercise at a high frequency. The brain weight did not change by a fast, and brain atrophy was not observed. Although no textural change was apparent by fasting, the neuronal neogenesis in the subventricular zone and hippocampus was inhibited, causing disorder of the brain function. A clear association between the suppression of encephalic neuropoiesis and overactivity was not established. However, it is interesting that the results of this study suggest that single short-term fasting has an effect on encephalic neuropoiesis.

Show MeSH
Related in: MedlinePlus