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Developmental switch in neurovascular coupling in the immature rodent barrel cortex.

Zehendner CM, Tsohataridis S, Luhmann HJ, Yang JW - PLoS ONE (2013)

Bottom Line: In the mature CNS it is well accepted that cortical sensory processing results in a rise in regional cerebral blood flow (rCBF).In contrast at P30, MUA remains stable during repetitive stimulation and is associated with an increase in rCBF.Further we characterize in both age groups the responses in NVC to single sensory stimuli.

View Article: PubMed Central - PubMed

Affiliation: Institute of Physiology, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany.

ABSTRACT
Neurovascular coupling (NVC) in the adult central nervous system (CNS) is a mechanism that provides regions of the brain with more oxygen and glucose upon increased levels of neural activation. Hemodynamic changes that go along with neural activation evoke a blood oxygen level-dependent (BOLD) signal in functional magnetic resonance imaging (fMRI) that can be used to study brain activity non-invasively. A correct correlation of the BOLD signal to neural activity is pivotal to understand this signal in neuronal development, health and disease. However, the function of NVC during development is largely unknown. The rodent whisker-to-barrel cortex is an experimentally well established model to study neurovascular interdependences. Using extracellular multi-electrode recordings and laser-Doppler-flowmetry (LDF) we show in the murine barrel cortex of postnatal day 7 (P7) and P30 mice in vivo that NVC undergoes a physiological shift during the first month of life. In the mature CNS it is well accepted that cortical sensory processing results in a rise in regional cerebral blood flow (rCBF). We show in P7 animals that rCBF decreases during prolonged multi-whisker stimulation and goes along with multi unit activity (MUA) fatigue. In contrast at P30, MUA remains stable during repetitive stimulation and is associated with an increase in rCBF. Further we characterize in both age groups the responses in NVC to single sensory stimuli. We suggest that the observed shift in NVC is an important process in cortical development that may be of high relevance for the correct interpretation of brain activity e.g. in fMRI studies of the immature central nervous system (CNS).

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Correlation of rCBF/MUA ratio and stimulation time.rCBF values were correlated to corresponding MUA and were plotted against stimulation time in arbitrary units (AU). rCBF per MUA increases over stimulation in P30 mice and is significantly positively correlated with stimulation duration (A). In contrast rCBF decreased per MUA over stimulation time in P7 mice. Here the correlation coefficient was negatively correlated (B). Error bars indicate + SEM.
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pone-0080749-g007: Correlation of rCBF/MUA ratio and stimulation time.rCBF values were correlated to corresponding MUA and were plotted against stimulation time in arbitrary units (AU). rCBF per MUA increases over stimulation in P30 mice and is significantly positively correlated with stimulation duration (A). In contrast rCBF decreased per MUA over stimulation time in P7 mice. Here the correlation coefficient was negatively correlated (B). Error bars indicate + SEM.

Mentions: Due to the observation of a highly prominent discrepancy in rCBF change with ongoing stimulation at 4 Hz we correlated rCBF changes to MUA activity at corresponding time points. Here we found a significant positive correlation in rCBF increase related to MUA over stimulation time in P30 mice (Pearson r = 0.9, P < 0.0001, n = 4 animals, Figure 7 A). That was in contrast to a highly significant negative correlation regarding rCBF/MUA ratio during stimulation in P7 mice (Spearman r = -0.9, P < 0.0001, n = 4 animals, Figure 7 B). Here rCBF per MUA decreased during stimulation.


Developmental switch in neurovascular coupling in the immature rodent barrel cortex.

Zehendner CM, Tsohataridis S, Luhmann HJ, Yang JW - PLoS ONE (2013)

Correlation of rCBF/MUA ratio and stimulation time.rCBF values were correlated to corresponding MUA and were plotted against stimulation time in arbitrary units (AU). rCBF per MUA increases over stimulation in P30 mice and is significantly positively correlated with stimulation duration (A). In contrast rCBF decreased per MUA over stimulation time in P7 mice. Here the correlation coefficient was negatively correlated (B). Error bars indicate + SEM.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3818260&req=5

pone-0080749-g007: Correlation of rCBF/MUA ratio and stimulation time.rCBF values were correlated to corresponding MUA and were plotted against stimulation time in arbitrary units (AU). rCBF per MUA increases over stimulation in P30 mice and is significantly positively correlated with stimulation duration (A). In contrast rCBF decreased per MUA over stimulation time in P7 mice. Here the correlation coefficient was negatively correlated (B). Error bars indicate + SEM.
Mentions: Due to the observation of a highly prominent discrepancy in rCBF change with ongoing stimulation at 4 Hz we correlated rCBF changes to MUA activity at corresponding time points. Here we found a significant positive correlation in rCBF increase related to MUA over stimulation time in P30 mice (Pearson r = 0.9, P < 0.0001, n = 4 animals, Figure 7 A). That was in contrast to a highly significant negative correlation regarding rCBF/MUA ratio during stimulation in P7 mice (Spearman r = -0.9, P < 0.0001, n = 4 animals, Figure 7 B). Here rCBF per MUA decreased during stimulation.

Bottom Line: In the mature CNS it is well accepted that cortical sensory processing results in a rise in regional cerebral blood flow (rCBF).In contrast at P30, MUA remains stable during repetitive stimulation and is associated with an increase in rCBF.Further we characterize in both age groups the responses in NVC to single sensory stimuli.

View Article: PubMed Central - PubMed

Affiliation: Institute of Physiology, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany.

ABSTRACT
Neurovascular coupling (NVC) in the adult central nervous system (CNS) is a mechanism that provides regions of the brain with more oxygen and glucose upon increased levels of neural activation. Hemodynamic changes that go along with neural activation evoke a blood oxygen level-dependent (BOLD) signal in functional magnetic resonance imaging (fMRI) that can be used to study brain activity non-invasively. A correct correlation of the BOLD signal to neural activity is pivotal to understand this signal in neuronal development, health and disease. However, the function of NVC during development is largely unknown. The rodent whisker-to-barrel cortex is an experimentally well established model to study neurovascular interdependences. Using extracellular multi-electrode recordings and laser-Doppler-flowmetry (LDF) we show in the murine barrel cortex of postnatal day 7 (P7) and P30 mice in vivo that NVC undergoes a physiological shift during the first month of life. In the mature CNS it is well accepted that cortical sensory processing results in a rise in regional cerebral blood flow (rCBF). We show in P7 animals that rCBF decreases during prolonged multi-whisker stimulation and goes along with multi unit activity (MUA) fatigue. In contrast at P30, MUA remains stable during repetitive stimulation and is associated with an increase in rCBF. Further we characterize in both age groups the responses in NVC to single sensory stimuli. We suggest that the observed shift in NVC is an important process in cortical development that may be of high relevance for the correct interpretation of brain activity e.g. in fMRI studies of the immature central nervous system (CNS).

Show MeSH
Related in: MedlinePlus